Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF250

Experiment: Ying_OrganicAcid7 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF248 and GFF249 are separated by 23 nucleotidesGFF249 and GFF250 are separated by 26 nucleotidesGFF250 and GFF251 are separated by 204 nucleotides GFF248 - Glycine oxidase ThiO (EC 1.4.3.19), at 264,424 to 265,506 GFF248 GFF249 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 265,530 to 266,378 GFF249 GFF250 - Predicted hydroxymethylpyrimidine transporter CytX, at 266,405 to 267,712 GFF250 GFF251 - hypothetical protein, at 267,917 to 268,714 GFF251 Position (kb) 266 267 268Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 265.560 kb on + strandat 265.560 kb on + strandat 265.560 kb on + strandat 265.560 kb on + strandat 265.560 kb on + strandat 265.560 kb on + strandat 265.843 kb on - strand, within GFF249at 266.218 kb on - strand, within GFF249at 266.268 kb on + strand, within GFF249at 266.269 kb on - strand, within GFF249at 266.269 kb on - strand, within GFF249at 266.304 kb on + strandat 266.305 kb on - strandat 266.529 kb on + strandat 266.530 kb on - strandat 266.530 kb on - strandat 266.530 kb on - strandat 266.530 kb on - strandat 266.631 kb on + strand, within GFF250at 266.631 kb on + strand, within GFF250at 266.632 kb on - strand, within GFF250at 266.632 kb on - strand, within GFF250at 266.928 kb on + strand, within GFF250at 266.928 kb on + strand, within GFF250at 266.929 kb on - strand, within GFF250at 266.952 kb on + strand, within GFF250at 267.435 kb on + strand, within GFF250at 267.436 kb on - strand, within GFF250at 267.436 kb on - strand, within GFF250at 267.436 kb on - strand, within GFF250at 267.576 kb on + strand, within GFF250at 267.810 kb on + strandat 267.811 kb on - strandat 267.811 kb on - strandat 267.811 kb on - strandat 267.909 kb on + strandat 267.910 kb on - strandat 267.911 kb on + strandat 268.041 kb on + strand, within GFF251at 268.041 kb on + strand, within GFF251at 268.041 kb on + strand, within GFF251at 268.041 kb on + strand, within GFF251at 268.041 kb on + strand, within GFF251at 268.041 kb on + strand, within GFF251at 268.042 kb on - strand, within GFF251at 268.042 kb on - strand, within GFF251at 268.042 kb on - strand, within GFF251at 268.042 kb on - strand, within GFF251at 268.162 kb on - strand, within GFF251at 268.317 kb on + strand, within GFF251at 268.317 kb on + strand, within GFF251at 268.318 kb on - strand, within GFF251at 268.318 kb on - strand, within GFF251at 268.318 kb on - strand, within GFF251at 268.318 kb on - strand, within GFF251at 268.509 kb on + strand, within GFF251at 268.587 kb on + strand, within GFF251at 268.588 kb on - strand, within GFF251at 268.588 kb on - strand, within GFF251

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 1
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265,560 + -1.7
265,560 + -1.7
265,560 + -3.5
265,560 + -0.2
265,560 + -1.1
265,560 + -4.0
265,843 - GFF249 0.37 -4.6
266,218 - GFF249 0.81 -3.6
266,268 + GFF249 0.87 -1.5
266,269 - GFF249 0.87 -0.6
266,269 - GFF249 0.87 -2.9
266,304 + -3.7
266,305 - -2.2
266,529 + -0.0
266,530 - +0.1
266,530 - -0.1
266,530 - -0.0
266,530 - -0.1
266,631 + GFF250 0.17 -0.3
266,631 + GFF250 0.17 +0.1
266,632 - GFF250 0.17 -2.6
266,632 - GFF250 0.17 -0.4
266,928 + GFF250 0.40 +0.7
266,928 + GFF250 0.40 -0.3
266,929 - GFF250 0.40 -0.3
266,952 + GFF250 0.42 +0.1
267,435 + GFF250 0.79 -0.7
267,436 - GFF250 0.79 +0.0
267,436 - GFF250 0.79 -1.0
267,436 - GFF250 0.79 +0.1
267,576 + GFF250 0.90 -0.2
267,810 + +0.3
267,811 - -0.4
267,811 - +0.1
267,811 - +0.0
267,909 + +0.0
267,910 - -0.9
267,911 + -1.0
268,041 + GFF251 0.16 +0.5
268,041 + GFF251 0.16 -0.4
268,041 + GFF251 0.16 +1.4
268,041 + GFF251 0.16 +0.4
268,041 + GFF251 0.16 -0.1
268,041 + GFF251 0.16 -0.4
268,042 - GFF251 0.16 -0.9
268,042 - GFF251 0.16 -0.5
268,042 - GFF251 0.16 -0.5
268,042 - GFF251 0.16 -0.2
268,162 - GFF251 0.31 -0.9
268,317 + GFF251 0.50 -0.1
268,317 + GFF251 0.50 +0.6
268,318 - GFF251 0.50 -1.8
268,318 - GFF251 0.50 +0.3
268,318 - GFF251 0.50 -0.3
268,318 - GFF251 0.50 +0.4
268,509 + GFF251 0.74 -0.2
268,587 + GFF251 0.84 -0.5
268,588 - GFF251 0.84 -1.6
268,588 - GFF251 0.84 -0.2

Or see this region's nucleotide sequence