Experiment: Ying_AminoAcid20 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5491 and GFF5492 overlap by 4 nucleotides GFF5492 and GFF5493 are separated by 118 nucleotides GFF5493 and GFF5494 are separated by 4 nucleotides GFF5494 and GFF5495 are separated by 16 nucleotides
GFF5491 - tRNA dimethylallyltransferase (EC 2.5.1.75), at 2,494,295 to 2,495,263
GFF5491
GFF5492 - AttE component of AttEFGH ABC transport system, at 2,495,260 to 2,495,928
GFF5492
GFF5493 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 2,496,047 to 2,497,522
GFF5493
GFF5494 - 6-phosphogluconolactonase (EC 3.1.1.31), eukaryotic type, at 2,497,527 to 2,498,267
GFF5494
GFF5495 - Phosphogluconate dehydratase (EC 4.2.1.12), at 2,498,284 to 2,500,167
GFF5495
Position (kb)
2496
2497
2498 Strain fitness (log2 ratio)
-1
0
1 at 2495.981 kb on + strand at 2496.036 kb on + strand at 2496.149 kb on + strand at 2496.149 kb on + strand at 2496.149 kb on + strand at 2496.149 kb on + strand at 2496.150 kb on - strand at 2496.150 kb on - strand at 2496.150 kb on - strand at 2496.150 kb on - strand at 2496.150 kb on - strand at 2496.150 kb on - strand at 2496.323 kb on + strand, within GFF5493 at 2496.323 kb on + strand, within GFF5493 at 2496.323 kb on + strand, within GFF5493 at 2496.324 kb on - strand, within GFF5493 at 2496.401 kb on + strand, within GFF5493 at 2496.401 kb on + strand, within GFF5493 at 2496.402 kb on - strand, within GFF5493 at 2496.404 kb on + strand, within GFF5493 at 2496.405 kb on - strand, within GFF5493 at 2496.555 kb on - strand, within GFF5493 at 2496.590 kb on + strand, within GFF5493 at 2496.590 kb on + strand, within GFF5493 at 2496.591 kb on - strand, within GFF5493 at 2496.591 kb on - strand, within GFF5493 at 2496.671 kb on + strand, within GFF5493 at 2496.671 kb on + strand, within GFF5493 at 2496.672 kb on - strand, within GFF5493 at 2496.735 kb on - strand, within GFF5493 at 2496.735 kb on - strand, within GFF5493 at 2496.920 kb on + strand, within GFF5493 at 2496.920 kb on + strand, within GFF5493 at 2496.920 kb on + strand, within GFF5493 at 2497.127 kb on + strand, within GFF5493 at 2497.127 kb on + strand, within GFF5493 at 2497.128 kb on - strand, within GFF5493 at 2497.308 kb on - strand, within GFF5493 at 2497.442 kb on + strand at 2498.176 kb on - strand, within GFF5494
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 1 remove 2,495,981 + +0.3 2,496,036 + -0.7 2,496,149 + -0.2 2,496,149 + +0.2 2,496,149 + -0.0 2,496,149 + +0.5 2,496,150 - -0.5 2,496,150 - +0.6 2,496,150 - +0.7 2,496,150 - +0.5 2,496,150 - -0.1 2,496,150 - +0.4 2,496,323 + GFF5493 0.19 -1.0 2,496,323 + GFF5493 0.19 +0.1 2,496,323 + GFF5493 0.19 -0.1 2,496,324 - GFF5493 0.19 +0.3 2,496,401 + GFF5493 0.24 -0.3 2,496,401 + GFF5493 0.24 -0.0 2,496,402 - GFF5493 0.24 -1.1 2,496,404 + GFF5493 0.24 -0.4 2,496,405 - GFF5493 0.24 +0.5 2,496,555 - GFF5493 0.34 +0.2 2,496,590 + GFF5493 0.37 -0.1 2,496,590 + GFF5493 0.37 +1.5 2,496,591 - GFF5493 0.37 -0.3 2,496,591 - GFF5493 0.37 -0.7 2,496,671 + GFF5493 0.42 -1.1 2,496,671 + GFF5493 0.42 -0.8 2,496,672 - GFF5493 0.42 -0.1 2,496,735 - GFF5493 0.47 -0.7 2,496,735 - GFF5493 0.47 -0.5 2,496,920 + GFF5493 0.59 -0.2 2,496,920 + GFF5493 0.59 -0.6 2,496,920 + GFF5493 0.59 -0.2 2,497,127 + GFF5493 0.73 -0.2 2,497,127 + GFF5493 0.73 +0.8 2,497,128 - GFF5493 0.73 -0.2 2,497,308 - GFF5493 0.85 +0.2 2,497,442 + -0.2 2,498,176 - GFF5494 0.88 +0.0
Or see this region's nucleotide sequence