Experiment: Ying_AminoAcid20 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3648 and GFF3649 are separated by 3 nucleotides GFF3649 and GFF3650 are separated by 63 nucleotides GFF3650 and GFF3651 are separated by 9 nucleotides GFF3651 and GFF3652 are separated by 3 nucleotides
GFF3648 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 533,200 to 536,487
GFF3648
GFF3649 - Inositol-1-monophosphatase (EC 3.1.3.25), at 536,491 to 537,375
GFF3649
GFF3650 - tRNA:Cm32/Um32 methyltransferase, at 537,439 to 538,254
GFF3650
GFF3651 - Serine acetyltransferase (EC 2.3.1.30), at 538,264 to 539,034
GFF3651
GFF3652 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 539,038 to 539,514
GFF3652
Position (kb) 537
538
539 Strain fitness (log2 ratio) -1
0
1 at 537.498 kb on + strand at 537.901 kb on + strand, within GFF3650 at 537.902 kb on - strand, within GFF3650 at 537.902 kb on - strand, within GFF3650 at 538.252 kb on + strand at 538.828 kb on + strand, within GFF3651 at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.033 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 1 remove 537,498 + +0.7 537,901 + GFF3650 0.57 +0.0 537,902 - GFF3650 0.57 +0.8 537,902 - GFF3650 0.57 +1.4 538,252 + +0.0 538,828 + GFF3651 0.73 -0.5 539,032 + +1.3 539,032 + -1.0 539,032 + -0.3 539,033 - -1.2
Or see this region's nucleotide sequence