Experiment: Ying_AminoAcid20 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1215 and GFF1216 overlap by 4 nucleotides GFF1216 and GFF1217 are separated by 26 nucleotides GFF1217 and GFF1218 are separated by 99 nucleotides
GFF1215 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85) / Aminodeoxychorismate lyase (EC 4.1.3.38), at 79,382 to 81,160
GFF1215
GFF1216 - Quinolinate synthetase (EC 2.5.1.72), at 81,157 to 82,269
GFF1216
GFF1217 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 82,296 to 83,159
GFF1217
GFF1218 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 83,259 to 84,119
GFF1218
Position (kb)
81
82
83 Strain fitness (log2 ratio)
-1
0
1
2 at 80.193 kb on + strand, within GFF1215 at 80.193 kb on + strand, within GFF1215 at 80.193 kb on + strand, within GFF1215 at 80.194 kb on - strand, within GFF1215 at 80.194 kb on - strand, within GFF1215 at 80.641 kb on - strand, within GFF1215 at 80.736 kb on + strand, within GFF1215 at 80.736 kb on + strand, within GFF1215 at 80.736 kb on + strand, within GFF1215 at 80.736 kb on + strand, within GFF1215 at 80.736 kb on + strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.737 kb on - strand, within GFF1215 at 80.958 kb on + strand, within GFF1215 at 80.958 kb on + strand, within GFF1215 at 80.959 kb on - strand, within GFF1215 at 80.959 kb on - strand, within GFF1215 at 81.687 kb on - strand, within GFF1216 at 81.687 kb on - strand, within GFF1216 at 81.687 kb on - strand, within GFF1216 at 81.687 kb on - strand, within GFF1216 at 81.821 kb on + strand, within GFF1216 at 81.821 kb on + strand, within GFF1216 at 81.822 kb on - strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.091 kb on + strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.092 kb on - strand, within GFF1216 at 82.094 kb on + strand, within GFF1216 at 82.095 kb on - strand, within GFF1216 at 82.095 kb on - strand, within GFF1216 at 82.229 kb on + strand at 82.230 kb on - strand at 82.263 kb on + strand at 82.263 kb on + strand at 82.264 kb on - strand at 82.455 kb on + strand, within GFF1217 at 82.456 kb on - strand, within GFF1217 at 82.456 kb on - strand, within GFF1217 at 82.456 kb on - strand, within GFF1217 at 82.661 kb on - strand, within GFF1217 at 82.705 kb on + strand, within GFF1217 at 82.706 kb on - strand, within GFF1217 at 82.706 kb on - strand, within GFF1217 at 83.185 kb on + strand at 83.185 kb on + strand at 83.186 kb on - strand at 83.186 kb on - strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.187 kb on + strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand at 83.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 1 remove 80,193 + GFF1215 0.46 -0.5 80,193 + GFF1215 0.46 -0.5 80,193 + GFF1215 0.46 +0.4 80,194 - GFF1215 0.46 +1.3 80,194 - GFF1215 0.46 +0.1 80,641 - GFF1215 0.71 -0.5 80,736 + GFF1215 0.76 +0.4 80,736 + GFF1215 0.76 +0.3 80,736 + GFF1215 0.76 -0.3 80,736 + GFF1215 0.76 -0.6 80,736 + GFF1215 0.76 -0.0 80,737 - GFF1215 0.76 -0.0 80,737 - GFF1215 0.76 -1.5 80,737 - GFF1215 0.76 -0.4 80,737 - GFF1215 0.76 -0.8 80,737 - GFF1215 0.76 -0.4 80,737 - GFF1215 0.76 +1.0 80,737 - GFF1215 0.76 -0.7 80,737 - GFF1215 0.76 -1.5 80,737 - GFF1215 0.76 +0.2 80,958 + GFF1215 0.89 -0.3 80,958 + GFF1215 0.89 +0.9 80,959 - GFF1215 0.89 -0.5 80,959 - GFF1215 0.89 +2.0 81,687 - GFF1216 0.48 +0.1 81,687 - GFF1216 0.48 -1.0 81,687 - GFF1216 0.48 -0.4 81,687 - GFF1216 0.48 +0.1 81,821 + GFF1216 0.60 +0.1 81,821 + GFF1216 0.60 -0.4 81,822 - GFF1216 0.60 -0.0 82,091 + GFF1216 0.84 +0.7 82,091 + GFF1216 0.84 +1.1 82,091 + GFF1216 0.84 +0.3 82,091 + GFF1216 0.84 -0.2 82,091 + GFF1216 0.84 +0.3 82,091 + GFF1216 0.84 -0.1 82,091 + GFF1216 0.84 +0.5 82,091 + GFF1216 0.84 -0.5 82,091 + GFF1216 0.84 -0.3 82,091 + GFF1216 0.84 +0.4 82,092 - GFF1216 0.84 +0.4 82,092 - GFF1216 0.84 -0.6 82,092 - GFF1216 0.84 -0.8 82,092 - GFF1216 0.84 -0.0 82,092 - GFF1216 0.84 +1.9 82,092 - GFF1216 0.84 +0.5 82,092 - GFF1216 0.84 +0.3 82,092 - GFF1216 0.84 +0.3 82,092 - GFF1216 0.84 +0.1 82,094 + GFF1216 0.84 +0.4 82,095 - GFF1216 0.84 +0.3 82,095 - GFF1216 0.84 +0.4 82,229 + -0.3 82,230 - -1.5 82,263 + +0.2 82,263 + +0.8 82,264 - -0.0 82,455 + GFF1217 0.18 +0.1 82,456 - GFF1217 0.19 +0.1 82,456 - GFF1217 0.19 +1.0 82,456 - GFF1217 0.19 -0.5 82,661 - GFF1217 0.42 -0.1 82,705 + GFF1217 0.47 +0.2 82,706 - GFF1217 0.47 +0.6 82,706 - GFF1217 0.47 +2.2 83,185 + -0.1 83,185 + +0.2 83,186 - +0.3 83,186 - -0.1 83,187 + -0.0 83,187 + -0.5 83,187 + -0.2 83,187 + -0.3 83,187 + -0.1 83,187 + +0.0 83,187 + +1.1 83,188 - -0.8 83,188 - -0.2 83,188 - +0.3 83,188 - -0.2 83,188 - -0.4 83,188 - -0.2
Or see this region's nucleotide sequence