Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF482

Experiment: Ying_AminoAcid20 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF481 and GFF482 overlap by 4 nucleotidesGFF482 and GFF483 are separated by 5 nucleotides GFF481 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 145,459 to 146,631 GFF481 GFF482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 146,628 to 147,428 GFF482 GFF483 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 147,434 to 149,341 GFF483 Position (kb) 146 147 148Strain fitness (log2 ratio) -1 0 1at 145.652 kb on - strand, within GFF481at 145.652 kb on - strand, within GFF481at 145.657 kb on + strand, within GFF481at 145.657 kb on + strand, within GFF481at 146.111 kb on - strand, within GFF481at 146.173 kb on + strand, within GFF481at 146.372 kb on - strand, within GFF481at 146.390 kb on - strand, within GFF481at 146.736 kb on + strand, within GFF482at 148.224 kb on - strand, within GFF483

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1
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145,652 - GFF481 0.16 -0.4
145,652 - GFF481 0.16 +0.7
145,657 + GFF481 0.17 -0.1
145,657 + GFF481 0.17 +0.0
146,111 - GFF481 0.56 +0.8
146,173 + GFF481 0.61 -0.4
146,372 - GFF481 0.78 +0.8
146,390 - GFF481 0.79 -0.2
146,736 + GFF482 0.13 +0.6
148,224 - GFF483 0.41 +0.2

Or see this region's nucleotide sequence