Experiment: Ying_AminoAcid20 rep A; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3139 and GFF3140 overlap by 4 nucleotides GFF3140 and GFF3141 overlap by 4 nucleotides GFF3141 and GFF3142 are separated by 215 nucleotides GFF3142 and GFF3143 overlap by 4 nucleotides
GFF3139 - IncF plasmid conjugative transfer pilus assembly protein TraU, at 18,217 to 19,236
GFF3139
GFF3140 - IncF plasmid conjugative transfer pilus assembly protein TraW, at 19,233 to 19,883
GFF3140
GFF3141 - hypothetical protein, at 19,880 to 20,110
GFF3141
GFF3142 - Conjugative signal peptidase TrhF, at 20,326 to 20,937
GFF3142
GFF3143 - hypothetical protein, at 20,934 to 21,296
GFF3143
Position (kb) 19
20
21 Strain fitness (log2 ratio) -1
0
1 at 19.242 kb on + strand at 19.342 kb on + strand, within GFF3140 at 19.343 kb on - strand, within GFF3140 at 19.343 kb on - strand, within GFF3140 at 19.343 kb on - strand, within GFF3140 at 19.582 kb on + strand, within GFF3140 at 19.583 kb on - strand, within GFF3140 at 19.583 kb on - strand, within GFF3140 at 20.064 kb on + strand, within GFF3141 at 20.065 kb on - strand, within GFF3141 at 20.065 kb on - strand, within GFF3141 at 20.426 kb on + strand, within GFF3142 at 20.427 kb on - strand, within GFF3142 at 20.429 kb on + strand, within GFF3142 at 20.430 kb on - strand, within GFF3142 at 20.801 kb on + strand, within GFF3142 at 20.801 kb on + strand, within GFF3142
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 1 remove 19,242 + -0.3 19,342 + GFF3140 0.17 +0.2 19,343 - GFF3140 0.17 +0.7 19,343 - GFF3140 0.17 +0.2 19,343 - GFF3140 0.17 -0.8 19,582 + GFF3140 0.54 -0.7 19,583 - GFF3140 0.54 +0.6 19,583 - GFF3140 0.54 +0.0 20,064 + GFF3141 0.80 -1.1 20,065 - GFF3141 0.80 -0.9 20,065 - GFF3141 0.80 +0.3 20,426 + GFF3142 0.16 +0.1 20,427 - GFF3142 0.17 -1.1 20,429 + GFF3142 0.17 +0.6 20,430 - GFF3142 0.17 +1.5 20,801 + GFF3142 0.78 -0.3 20,801 + GFF3142 0.78 +0.3
Or see this region's nucleotide sequence