Experiment: Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaG and COO64_RS22520 overlap by 1 nucleotides COO64_RS22520 and glpE are separated by 14 nucleotides glpE and COO64_RS22530 are separated by 281 nucleotides
COO64_RS22515: apaG - Co2+/Mg2+ efflux protein ApaG, at 38,849 to 39,229
apaG
COO64_RS22520: COO64_RS22520 - symmetrical bis(5'-nucleosyl)-tetraphosphatase, at 39,229 to 40,104
_RS22520
COO64_RS22525: glpE - thiosulfate sulfurtransferase GlpE, at 40,119 to 40,448
glpE
COO64_RS22530: COO64_RS22530 - PrkA family serine protein kinase, at 40,730 to 42,652
_RS22530
Position (kb) 40
41 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 39.146 kb on + strand, within apaG at 39.146 kb on + strand, within apaG at 39.147 kb on - strand, within apaG at 39.169 kb on + strand, within apaG at 39.238 kb on + strand at 39.683 kb on - strand, within COO64_RS22520 at 40.083 kb on + strand at 40.084 kb on - strand at 40.209 kb on + strand, within glpE at 40.209 kb on + strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.210 kb on - strand, within glpE at 40.311 kb on + strand, within glpE at 40.312 kb on - strand, within glpE at 40.344 kb on + strand, within glpE at 40.344 kb on + strand, within glpE at 40.345 kb on - strand, within glpE at 40.374 kb on + strand, within glpE at 40.375 kb on - strand, within glpE at 40.375 kb on - strand, within glpE at 40.375 kb on - strand, within glpE at 40.414 kb on - strand, within glpE at 40.525 kb on + strand at 40.525 kb on + strand at 40.525 kb on + strand at 40.525 kb on + strand at 40.525 kb on + strand at 40.526 kb on - strand at 40.526 kb on - strand at 40.550 kb on + strand at 40.557 kb on + strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.558 kb on - strand at 40.564 kb on - strand at 40.564 kb on - strand at 40.596 kb on + strand at 40.596 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.629 kb on + strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.630 kb on - strand at 40.645 kb on + strand at 40.645 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.647 kb on + strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.648 kb on - strand at 40.711 kb on + strand at 40.735 kb on + strand at 40.735 kb on + strand at 40.736 kb on - strand at 40.736 kb on - strand at 40.769 kb on - strand at 40.799 kb on + strand at 40.800 kb on - strand at 40.800 kb on - strand at 40.832 kb on + strand at 40.832 kb on + strand at 40.832 kb on + strand at 40.832 kb on + strand at 40.833 kb on - strand at 40.833 kb on - strand at 41.000 kb on + strand, within COO64_RS22530 at 41.000 kb on + strand, within COO64_RS22530 at 41.000 kb on + strand, within COO64_RS22530 at 41.000 kb on + strand, within COO64_RS22530 at 41.048 kb on + strand, within COO64_RS22530 at 41.049 kb on - strand, within COO64_RS22530 at 41.055 kb on + strand, within COO64_RS22530 at 41.056 kb on - strand, within COO64_RS22530 at 41.056 kb on - strand, within COO64_RS22530 at 41.056 kb on - strand, within COO64_RS22530 at 41.074 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.126 kb on + strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.127 kb on - strand, within COO64_RS22530 at 41.228 kb on + strand, within COO64_RS22530 at 41.316 kb on - strand, within COO64_RS22530 at 41.380 kb on + strand, within COO64_RS22530 at 41.380 kb on + strand, within COO64_RS22530 at 41.380 kb on + strand, within COO64_RS22530 at 41.381 kb on - strand, within COO64_RS22530 at 41.426 kb on + strand, within COO64_RS22530 at 41.426 kb on + strand, within COO64_RS22530 at 41.426 kb on + strand, within COO64_RS22530 at 41.427 kb on - strand, within COO64_RS22530
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9 remove 39,146 + apaG COO64_RS22515 0.78 +0.5 39,146 + apaG COO64_RS22515 0.78 +0.4 39,147 - apaG COO64_RS22515 0.78 -2.2 39,169 + apaG COO64_RS22515 0.84 -1.4 39,238 + -0.5 39,683 - COO64_RS22520 0.52 -0.4 40,083 + -0.3 40,084 - -0.2 40,209 + glpE COO64_RS22525 0.27 +0.4 40,209 + glpE COO64_RS22525 0.27 +0.7 40,210 - glpE COO64_RS22525 0.28 +0.1 40,210 - glpE COO64_RS22525 0.28 -0.2 40,210 - glpE COO64_RS22525 0.28 -0.3 40,210 - glpE COO64_RS22525 0.28 -0.3 40,210 - glpE COO64_RS22525 0.28 -0.9 40,210 - glpE COO64_RS22525 0.28 +0.3 40,210 - glpE COO64_RS22525 0.28 -1.3 40,210 - glpE COO64_RS22525 0.28 +0.1 40,311 + glpE COO64_RS22525 0.58 +0.1 40,312 - glpE COO64_RS22525 0.58 -1.3 40,344 + glpE COO64_RS22525 0.68 +0.2 40,344 + glpE COO64_RS22525 0.68 -0.3 40,345 - glpE COO64_RS22525 0.68 -0.9 40,374 + glpE COO64_RS22525 0.77 -1.1 40,375 - glpE COO64_RS22525 0.78 +0.1 40,375 - glpE COO64_RS22525 0.78 -0.2 40,375 - glpE COO64_RS22525 0.78 -0.1 40,414 - glpE COO64_RS22525 0.89 -1.1 40,525 + -0.2 40,525 + +1.1 40,525 + +0.4 40,525 + -0.6 40,525 + -0.4 40,526 - +0.0 40,526 - +0.4 40,550 + -0.1 40,557 + -0.1 40,558 - +0.8 40,558 - +0.1 40,558 - +0.8 40,558 - -0.1 40,558 - -0.6 40,564 - +3.2 40,564 - +0.1 40,596 + +0.1 40,596 + +0.5 40,629 + +0.2 40,629 + +0.5 40,629 + -1.2 40,630 - +0.4 40,630 - -0.9 40,630 - +0.1 40,630 - +1.9 40,645 + -0.3 40,645 + +0.2 40,647 + -0.3 40,647 + +0.6 40,647 + -0.1 40,647 + +0.0 40,647 + +0.4 40,647 + -1.0 40,647 + -0.8 40,647 + +0.9 40,647 + +1.1 40,647 + +0.3 40,648 - -0.3 40,648 - +1.0 40,648 - -0.5 40,648 - +0.6 40,648 - +1.0 40,648 - +1.1 40,648 - -0.8 40,648 - +0.2 40,648 - +0.3 40,648 - -0.1 40,648 - +0.6 40,648 - -0.0 40,648 - -0.9 40,648 - +0.1 40,648 - +0.5 40,648 - -1.4 40,648 - +0.4 40,711 + -0.3 40,735 + -0.1 40,735 + -0.2 40,736 - -0.6 40,736 - -0.8 40,769 - -1.3 40,799 + +0.1 40,800 - -0.2 40,800 - -0.6 40,832 + -0.9 40,832 + +0.0 40,832 + -0.5 40,832 + -0.4 40,833 - -0.0 40,833 - +0.8 41,000 + COO64_RS22530 0.14 -0.1 41,000 + COO64_RS22530 0.14 +0.2 41,000 + COO64_RS22530 0.14 -1.0 41,000 + COO64_RS22530 0.14 +0.5 41,048 + COO64_RS22530 0.17 +0.2 41,049 - COO64_RS22530 0.17 +0.4 41,055 + COO64_RS22530 0.17 +0.9 41,056 - COO64_RS22530 0.17 -0.4 41,056 - COO64_RS22530 0.17 -0.6 41,056 - COO64_RS22530 0.17 +0.1 41,074 + COO64_RS22530 0.18 +0.6 41,126 + COO64_RS22530 0.21 -0.7 41,126 + COO64_RS22530 0.21 +0.7 41,126 + COO64_RS22530 0.21 -0.6 41,126 + COO64_RS22530 0.21 +0.3 41,126 + COO64_RS22530 0.21 -0.4 41,126 + COO64_RS22530 0.21 +0.3 41,126 + COO64_RS22530 0.21 +1.3 41,126 + COO64_RS22530 0.21 -0.3 41,126 + COO64_RS22530 0.21 +0.2 41,126 + COO64_RS22530 0.21 -0.7 41,126 + COO64_RS22530 0.21 +0.1 41,127 - COO64_RS22530 0.21 -0.5 41,127 - COO64_RS22530 0.21 -0.7 41,127 - COO64_RS22530 0.21 +0.6 41,127 - COO64_RS22530 0.21 -0.1 41,127 - COO64_RS22530 0.21 -0.4 41,127 - COO64_RS22530 0.21 -1.3 41,127 - COO64_RS22530 0.21 +0.7 41,127 - COO64_RS22530 0.21 +0.0 41,127 - COO64_RS22530 0.21 +0.1 41,127 - COO64_RS22530 0.21 -0.9 41,127 - COO64_RS22530 0.21 -0.0 41,127 - COO64_RS22530 0.21 -0.3 41,228 + COO64_RS22530 0.26 -0.0 41,316 - COO64_RS22530 0.30 -0.8 41,380 + COO64_RS22530 0.34 +0.5 41,380 + COO64_RS22530 0.34 -1.1 41,380 + COO64_RS22530 0.34 +0.6 41,381 - COO64_RS22530 0.34 +0.1 41,426 + COO64_RS22530 0.36 +1.6 41,426 + COO64_RS22530 0.36 -0.1 41,426 + COO64_RS22530 0.36 -0.9 41,427 - COO64_RS22530 0.36 +0.6
Or see this region's nucleotide sequence