Experiment: Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadA and COO64_RS20220 are separated by 69 nucleotides COO64_RS20220 and COO64_RS20225 are separated by 124 nucleotides COO64_RS20225 and COO64_RS20230 are separated by 24 nucleotides
COO64_RS20215: nadA - quinolinate synthase NadA, at 147,345 to 148,403
nadA
COO64_RS20220: COO64_RS20220 - M48 family metalloprotease, at 148,473 to 149,906
_RS20220
COO64_RS20225: COO64_RS20225 - sulfurtransferase TusA family protein, at 150,031 to 150,270
_RS20225
COO64_RS20230: COO64_RS20230 - AI-2E family transporter, at 150,295 to 151,365
_RS20230
Position (kb) 148
149
150 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.489 kb on + strand, within nadA at 147.490 kb on - strand, within nadA at 147.490 kb on - strand, within nadA at 147.490 kb on - strand, within nadA at 147.492 kb on + strand, within nadA at 147.492 kb on + strand, within nadA at 147.492 kb on + strand, within nadA at 147.492 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.762 kb on + strand, within nadA at 147.763 kb on - strand, within nadA at 147.763 kb on - strand, within nadA at 147.763 kb on - strand, within nadA at 147.763 kb on - strand, within nadA at 147.897 kb on + strand, within nadA at 147.897 kb on + strand, within nadA at 147.897 kb on + strand, within nadA at 147.898 kb on - strand, within nadA at 147.898 kb on - strand, within nadA at 147.898 kb on - strand, within nadA at 147.898 kb on - strand, within nadA at 148.149 kb on + strand, within nadA at 148.149 kb on + strand, within nadA at 148.149 kb on + strand, within nadA at 148.150 kb on - strand, within nadA at 148.150 kb on - strand, within nadA at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.352 kb on + strand at 148.353 kb on - strand at 148.353 kb on - strand at 148.353 kb on - strand at 148.353 kb on - strand at 148.433 kb on - strand at 148.433 kb on - strand at 148.438 kb on - strand at 148.438 kb on - strand at 148.438 kb on - strand at 148.574 kb on + strand at 148.627 kb on + strand, within COO64_RS20220 at 148.627 kb on + strand, within COO64_RS20220 at 148.628 kb on - strand, within COO64_RS20220 at 148.693 kb on + strand, within COO64_RS20220 at 148.807 kb on + strand, within COO64_RS20220 at 148.807 kb on + strand, within COO64_RS20220 at 148.808 kb on - strand, within COO64_RS20220 at 148.808 kb on - strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.903 kb on + strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 148.904 kb on - strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.077 kb on + strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.078 kb on - strand, within COO64_RS20220 at 149.227 kb on + strand, within COO64_RS20220 at 149.228 kb on - strand, within COO64_RS20220 at 149.228 kb on - strand, within COO64_RS20220 at 149.234 kb on - strand, within COO64_RS20220 at 149.535 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.545 kb on + strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.546 kb on - strand, within COO64_RS20220 at 149.667 kb on + strand, within COO64_RS20220 at 149.667 kb on + strand, within COO64_RS20220 at 149.668 kb on - strand, within COO64_RS20220 at 149.710 kb on + strand, within COO64_RS20220 at 149.711 kb on - strand, within COO64_RS20220 at 149.935 kb on + strand at 149.935 kb on + strand at 149.935 kb on + strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.936 kb on - strand at 149.999 kb on - strand at 149.999 kb on - strand at 150.210 kb on + strand, within COO64_RS20225 at 150.210 kb on + strand, within COO64_RS20225 at 150.210 kb on + strand, within COO64_RS20225 at 150.210 kb on + strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.211 kb on - strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.244 kb on + strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.245 kb on - strand, within COO64_RS20225 at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.250 kb on + strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.251 kb on - strand at 150.328 kb on + strand at 150.328 kb on + strand at 150.328 kb on + strand at 150.329 kb on - strand at 150.329 kb on - strand at 150.353 kb on + strand at 150.354 kb on - strand at 150.787 kb on + strand, within COO64_RS20230 at 150.787 kb on + strand, within COO64_RS20230 at 150.787 kb on + strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.788 kb on - strand, within COO64_RS20230 at 150.862 kb on + strand, within COO64_RS20230
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9 remove 147,489 + nadA COO64_RS20215 0.14 -0.9 147,489 + nadA COO64_RS20215 0.14 -1.0 147,489 + nadA COO64_RS20215 0.14 -1.6 147,489 + nadA COO64_RS20215 0.14 +0.8 147,489 + nadA COO64_RS20215 0.14 -0.5 147,489 + nadA COO64_RS20215 0.14 -0.2 147,490 - nadA COO64_RS20215 0.14 -0.7 147,490 - nadA COO64_RS20215 0.14 -1.5 147,490 - nadA COO64_RS20215 0.14 -0.6 147,492 + nadA COO64_RS20215 0.14 -1.2 147,492 + nadA COO64_RS20215 0.14 -0.9 147,492 + nadA COO64_RS20215 0.14 -1.7 147,492 + nadA COO64_RS20215 0.14 +0.2 147,762 + nadA COO64_RS20215 0.39 -0.2 147,762 + nadA COO64_RS20215 0.39 -0.4 147,762 + nadA COO64_RS20215 0.39 +0.6 147,762 + nadA COO64_RS20215 0.39 +0.5 147,762 + nadA COO64_RS20215 0.39 -1.3 147,762 + nadA COO64_RS20215 0.39 -0.7 147,762 + nadA COO64_RS20215 0.39 -1.3 147,763 - nadA COO64_RS20215 0.39 -0.7 147,763 - nadA COO64_RS20215 0.39 -2.0 147,763 - nadA COO64_RS20215 0.39 -0.6 147,763 - nadA COO64_RS20215 0.39 -2.1 147,897 + nadA COO64_RS20215 0.52 -0.5 147,897 + nadA COO64_RS20215 0.52 +1.3 147,897 + nadA COO64_RS20215 0.52 -0.5 147,898 - nadA COO64_RS20215 0.52 -0.1 147,898 - nadA COO64_RS20215 0.52 +0.8 147,898 - nadA COO64_RS20215 0.52 -0.7 147,898 - nadA COO64_RS20215 0.52 +0.5 148,149 + nadA COO64_RS20215 0.76 -1.6 148,149 + nadA COO64_RS20215 0.76 +0.1 148,149 + nadA COO64_RS20215 0.76 -0.8 148,150 - nadA COO64_RS20215 0.76 -2.4 148,150 - nadA COO64_RS20215 0.76 -0.5 148,352 + -0.2 148,352 + -0.4 148,352 + -1.6 148,352 + +0.1 148,352 + -0.0 148,352 + -0.5 148,352 + -1.2 148,353 - -1.1 148,353 - +0.4 148,353 - -0.5 148,353 - +0.5 148,433 - -0.2 148,433 - -0.5 148,438 - +0.3 148,438 - -0.9 148,438 - -0.5 148,574 + +0.2 148,627 + COO64_RS20220 0.11 +0.7 148,627 + COO64_RS20220 0.11 +0.0 148,628 - COO64_RS20220 0.11 +1.0 148,693 + COO64_RS20220 0.15 +0.1 148,807 + COO64_RS20220 0.23 -0.8 148,807 + COO64_RS20220 0.23 -0.4 148,808 - COO64_RS20220 0.23 -0.0 148,808 - COO64_RS20220 0.23 -1.2 148,903 + COO64_RS20220 0.30 -0.4 148,903 + COO64_RS20220 0.30 -0.0 148,903 + COO64_RS20220 0.30 -1.9 148,903 + COO64_RS20220 0.30 +1.4 148,903 + COO64_RS20220 0.30 +0.8 148,903 + COO64_RS20220 0.30 +1.0 148,903 + COO64_RS20220 0.30 +0.5 148,904 - COO64_RS20220 0.30 -1.1 148,904 - COO64_RS20220 0.30 +0.4 148,904 - COO64_RS20220 0.30 -0.0 148,904 - COO64_RS20220 0.30 -0.4 148,904 - COO64_RS20220 0.30 -0.5 148,904 - COO64_RS20220 0.30 +0.7 149,077 + COO64_RS20220 0.42 -0.7 149,077 + COO64_RS20220 0.42 -0.3 149,077 + COO64_RS20220 0.42 +0.4 149,077 + COO64_RS20220 0.42 -0.0 149,077 + COO64_RS20220 0.42 -1.4 149,078 - COO64_RS20220 0.42 -1.8 149,078 - COO64_RS20220 0.42 -0.0 149,078 - COO64_RS20220 0.42 +2.1 149,078 - COO64_RS20220 0.42 +0.9 149,227 + COO64_RS20220 0.53 +0.4 149,228 - COO64_RS20220 0.53 +0.0 149,228 - COO64_RS20220 0.53 +0.0 149,234 - COO64_RS20220 0.53 +0.9 149,535 + COO64_RS20220 0.74 -0.7 149,545 + COO64_RS20220 0.75 +0.1 149,545 + COO64_RS20220 0.75 -0.7 149,545 + COO64_RS20220 0.75 -0.5 149,545 + COO64_RS20220 0.75 +0.5 149,545 + COO64_RS20220 0.75 -0.2 149,546 - COO64_RS20220 0.75 -0.6 149,546 - COO64_RS20220 0.75 -2.0 149,546 - COO64_RS20220 0.75 +1.1 149,546 - COO64_RS20220 0.75 -0.4 149,546 - COO64_RS20220 0.75 -1.4 149,546 - COO64_RS20220 0.75 +1.0 149,667 + COO64_RS20220 0.83 +0.3 149,667 + COO64_RS20220 0.83 +0.8 149,668 - COO64_RS20220 0.83 +0.5 149,710 + COO64_RS20220 0.86 +0.2 149,711 - COO64_RS20220 0.86 +0.5 149,935 + -0.2 149,935 + +0.7 149,935 + +0.5 149,936 - -0.1 149,936 - +0.1 149,936 - -0.5 149,936 - +0.2 149,936 - +0.0 149,936 - -0.5 149,999 - +0.2 149,999 - +1.8 150,210 + COO64_RS20225 0.75 -0.6 150,210 + COO64_RS20225 0.75 -1.5 150,210 + COO64_RS20225 0.75 +0.3 150,210 + COO64_RS20225 0.75 -0.4 150,211 - COO64_RS20225 0.75 +0.8 150,211 - COO64_RS20225 0.75 +1.1 150,211 - COO64_RS20225 0.75 -1.0 150,211 - COO64_RS20225 0.75 +1.5 150,211 - COO64_RS20225 0.75 -0.9 150,211 - COO64_RS20225 0.75 -0.8 150,211 - COO64_RS20225 0.75 -0.9 150,211 - COO64_RS20225 0.75 -1.4 150,211 - COO64_RS20225 0.75 -0.4 150,211 - COO64_RS20225 0.75 -2.7 150,244 + COO64_RS20225 0.89 -0.9 150,244 + COO64_RS20225 0.89 -1.2 150,244 + COO64_RS20225 0.89 -0.7 150,244 + COO64_RS20225 0.89 -0.5 150,244 + COO64_RS20225 0.89 -0.5 150,244 + COO64_RS20225 0.89 +1.5 150,244 + COO64_RS20225 0.89 -1.9 150,244 + COO64_RS20225 0.89 -0.3 150,244 + COO64_RS20225 0.89 -0.0 150,244 + COO64_RS20225 0.89 -2.0 150,244 + COO64_RS20225 0.89 -0.1 150,245 - COO64_RS20225 0.89 -0.9 150,245 - COO64_RS20225 0.89 -0.8 150,245 - COO64_RS20225 0.89 +1.5 150,245 - COO64_RS20225 0.89 -1.5 150,245 - COO64_RS20225 0.89 -1.7 150,245 - COO64_RS20225 0.89 -1.9 150,245 - COO64_RS20225 0.89 -0.2 150,245 - COO64_RS20225 0.89 -2.3 150,245 - COO64_RS20225 0.89 -0.8 150,250 + -1.9 150,250 + -1.4 150,250 + -1.9 150,250 + +0.5 150,250 + -2.5 150,250 + -0.0 150,250 + -1.4 150,250 + -2.8 150,250 + -0.5 150,251 - -0.8 150,251 - +0.9 150,251 - -1.3 150,251 - -2.0 150,251 - -0.5 150,328 + -0.0 150,328 + -0.1 150,328 + +1.1 150,329 - +0.9 150,329 - -0.0 150,353 + +0.5 150,354 - -0.5 150,787 + COO64_RS20230 0.46 -1.0 150,787 + COO64_RS20230 0.46 +0.6 150,787 + COO64_RS20230 0.46 +0.5 150,788 - COO64_RS20230 0.46 +0.5 150,788 - COO64_RS20230 0.46 -0.7 150,788 - COO64_RS20230 0.46 -0.8 150,788 - COO64_RS20230 0.46 +1.2 150,788 - COO64_RS20230 0.46 +0.3 150,788 - COO64_RS20230 0.46 -0.0 150,788 - COO64_RS20230 0.46 -0.3 150,788 - COO64_RS20230 0.46 +0.3 150,862 + COO64_RS20230 0.53 +1.1
Or see this region's nucleotide sequence