Experiment: Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pal and ybgF are separated by 6 nucleotides ybgF and queE are separated by 158 nucleotides queE and queC are separated by 13 nucleotides queC and COO64_RS20150 are separated by 61 nucleotides COO64_RS20150 and COO64_RS20155 are separated by 134 nucleotides
COO64_RS20130: pal - peptidoglycan-associated lipoprotein Pal, at 129,810 to 130,307
pal
COO64_RS20135: ybgF - tol-pal system protein YbgF, at 130,314 to 131,144
ybgF
COO64_RS20140: queE - 7-carboxy-7-deazaguanine synthase QueE, at 131,303 to 131,950
queE
COO64_RS20145: queC - 7-cyano-7-deazaguanine synthase QueC, at 131,964 to 132,638
queC
COO64_RS20150: COO64_RS20150 - tRNA-Lys, at 132,700 to 132,775
_RS20150
COO64_RS20155: COO64_RS20155 - TonB-dependent siderophore receptor, at 132,910 to 135,123
_RS20155
Position (kb) 131
132 Strain fitness (log2 ratio) -2
-1
0
1
2 at 130.305 kb on + strand at 130.306 kb on - strand at 130.306 kb on - strand at 130.336 kb on + strand at 130.407 kb on + strand, within ybgF at 130.408 kb on - strand, within ybgF at 130.408 kb on - strand, within ybgF at 130.409 kb on + strand, within ybgF at 130.440 kb on + strand, within ybgF at 130.579 kb on + strand, within ybgF at 130.579 kb on + strand, within ybgF at 130.579 kb on + strand, within ybgF at 130.579 kb on + strand, within ybgF at 130.579 kb on + strand, within ybgF at 130.579 kb on + strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.580 kb on - strand, within ybgF at 130.645 kb on + strand, within ybgF at 130.645 kb on + strand, within ybgF at 130.645 kb on + strand, within ybgF at 130.645 kb on + strand, within ybgF at 130.645 kb on + strand, within ybgF at 130.646 kb on - strand, within ybgF at 130.646 kb on - strand, within ybgF at 130.646 kb on - strand, within ybgF at 130.788 kb on + strand, within ybgF at 130.789 kb on - strand, within ybgF at 130.789 kb on - strand, within ybgF at 130.791 kb on + strand, within ybgF at 130.791 kb on + strand, within ybgF at 130.792 kb on - strand, within ybgF at 130.792 kb on - strand, within ybgF at 130.870 kb on - strand, within ybgF at 130.870 kb on - strand, within ybgF at 130.884 kb on + strand, within ybgF at 130.885 kb on - strand, within ybgF at 130.919 kb on - strand, within ybgF at 130.980 kb on + strand, within ybgF at 130.981 kb on - strand, within ybgF at 130.981 kb on - strand, within ybgF at 131.013 kb on + strand, within ybgF at 131.013 kb on + strand, within ybgF at 131.013 kb on + strand, within ybgF at 131.014 kb on - strand, within ybgF at 131.029 kb on + strand, within ybgF at 131.029 kb on + strand, within ybgF at 131.029 kb on + strand, within ybgF at 131.029 kb on + strand, within ybgF at 131.029 kb on + strand, within ybgF at 131.029 kb on + strand, within ybgF at 131.030 kb on - strand, within ybgF at 131.030 kb on - strand, within ybgF at 131.030 kb on - strand, within ybgF at 131.030 kb on - strand, within ybgF at 131.209 kb on - strand at 131.230 kb on + strand at 131.280 kb on + strand at 131.280 kb on + strand at 131.280 kb on + strand at 131.281 kb on - strand at 131.281 kb on - strand at 131.281 kb on - strand at 131.302 kb on + strand at 131.302 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.316 kb on + strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.317 kb on - strand at 131.320 kb on + strand at 131.320 kb on + strand at 131.320 kb on + strand at 131.320 kb on + strand at 131.321 kb on - strand at 131.321 kb on - strand at 131.417 kb on + strand, within queE at 131.432 kb on + strand, within queE at 131.432 kb on + strand, within queE at 131.433 kb on - strand, within queE at 131.433 kb on - strand, within queE at 131.493 kb on - strand, within queE at 131.493 kb on - strand, within queE at 131.493 kb on - strand, within queE at 131.504 kb on + strand, within queE at 131.583 kb on - strand, within queE at 131.618 kb on - strand, within queE at 131.618 kb on - strand, within queE at 131.743 kb on + strand, within queE at 131.744 kb on - strand, within queE at 131.762 kb on + strand, within queE at 131.762 kb on + strand, within queE at 131.762 kb on + strand, within queE at 131.763 kb on - strand, within queE at 131.763 kb on - strand, within queE at 131.983 kb on + strand at 132.046 kb on - strand, within queC at 132.046 kb on - strand, within queC at 132.046 kb on - strand, within queC at 132.047 kb on + strand, within queC at 132.047 kb on + strand, within queC at 132.047 kb on + strand, within queC at 132.047 kb on + strand, within queC at 132.047 kb on + strand, within queC at 132.047 kb on + strand, within queC at 132.048 kb on - strand, within queC at 132.048 kb on - strand, within queC at 132.048 kb on - strand, within queC at 132.054 kb on + strand, within queC at 132.054 kb on + strand, within queC at 132.055 kb on - strand, within queC at 132.055 kb on - strand, within queC at 132.055 kb on - strand, within queC at 132.055 kb on - strand, within queC at 132.055 kb on - strand, within queC at 132.055 kb on - strand, within queC at 132.055 kb on - strand, within queC at 132.170 kb on + strand, within queC at 132.209 kb on - strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.243 kb on + strand, within queC at 132.244 kb on - strand, within queC at 132.244 kb on - strand, within queC at 132.244 kb on - strand, within queC at 132.260 kb on + strand, within queC at 132.260 kb on + strand, within queC at 132.260 kb on + strand, within queC at 132.261 kb on - strand, within queC at 132.261 kb on - strand, within queC at 132.315 kb on - strand, within queC at 132.342 kb on + strand, within queC at 132.343 kb on - strand, within queC at 132.510 kb on + strand, within queC at 132.511 kb on - strand, within queC at 132.531 kb on + strand, within queC at 132.613 kb on + strand at 132.749 kb on + strand, within COO64_RS20150 at 132.749 kb on + strand, within COO64_RS20150 at 132.749 kb on + strand, within COO64_RS20150 at 132.776 kb on + strand at 132.776 kb on + strand at 132.776 kb on + strand at 132.783 kb on + strand at 132.783 kb on + strand at 132.860 kb on - strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.870 kb on + strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.871 kb on - strand at 132.904 kb on + strand at 132.904 kb on + strand at 132.904 kb on + strand at 132.905 kb on - strand at 132.905 kb on - strand at 132.905 kb on - strand at 132.905 kb on - strand at 132.920 kb on + strand at 132.920 kb on + strand at 132.920 kb on + strand at 132.921 kb on - strand at 132.921 kb on - strand at 132.921 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Mixed culture; Pseudomonas DC3000 1:1 with Pseudomonas_SVBP6_ML3 in M9 remove 130,305 + +0.5 130,306 - -0.0 130,306 - -0.5 130,336 + -0.9 130,407 + ybgF COO64_RS20135 0.11 +0.1 130,408 - ybgF COO64_RS20135 0.11 -1.0 130,408 - ybgF COO64_RS20135 0.11 +1.5 130,409 + ybgF COO64_RS20135 0.11 +0.1 130,440 + ybgF COO64_RS20135 0.15 +1.0 130,579 + ybgF COO64_RS20135 0.32 -1.6 130,579 + ybgF COO64_RS20135 0.32 +0.5 130,579 + ybgF COO64_RS20135 0.32 -0.8 130,579 + ybgF COO64_RS20135 0.32 -0.0 130,579 + ybgF COO64_RS20135 0.32 +1.4 130,579 + ybgF COO64_RS20135 0.32 -0.3 130,580 - ybgF COO64_RS20135 0.32 +0.5 130,580 - ybgF COO64_RS20135 0.32 +1.0 130,580 - ybgF COO64_RS20135 0.32 +0.3 130,580 - ybgF COO64_RS20135 0.32 +0.1 130,580 - ybgF COO64_RS20135 0.32 +0.7 130,580 - ybgF COO64_RS20135 0.32 -1.9 130,580 - ybgF COO64_RS20135 0.32 +0.4 130,580 - ybgF COO64_RS20135 0.32 +0.5 130,580 - ybgF COO64_RS20135 0.32 +1.0 130,580 - ybgF COO64_RS20135 0.32 -0.2 130,645 + ybgF COO64_RS20135 0.40 +0.1 130,645 + ybgF COO64_RS20135 0.40 -0.3 130,645 + ybgF COO64_RS20135 0.40 +0.9 130,645 + ybgF COO64_RS20135 0.40 +0.3 130,645 + ybgF COO64_RS20135 0.40 +0.1 130,646 - ybgF COO64_RS20135 0.40 -1.0 130,646 - ybgF COO64_RS20135 0.40 -0.6 130,646 - ybgF COO64_RS20135 0.40 -0.4 130,788 + ybgF COO64_RS20135 0.57 -0.1 130,789 - ybgF COO64_RS20135 0.57 +0.3 130,789 - ybgF COO64_RS20135 0.57 +0.3 130,791 + ybgF COO64_RS20135 0.57 +0.3 130,791 + ybgF COO64_RS20135 0.57 +0.3 130,792 - ybgF COO64_RS20135 0.58 +0.3 130,792 - ybgF COO64_RS20135 0.58 -0.9 130,870 - ybgF COO64_RS20135 0.67 +0.4 130,870 - ybgF COO64_RS20135 0.67 -1.4 130,884 + ybgF COO64_RS20135 0.69 -0.3 130,885 - ybgF COO64_RS20135 0.69 +0.4 130,919 - ybgF COO64_RS20135 0.73 -1.2 130,980 + ybgF COO64_RS20135 0.80 +1.1 130,981 - ybgF COO64_RS20135 0.80 +0.5 130,981 - ybgF COO64_RS20135 0.80 -0.1 131,013 + ybgF COO64_RS20135 0.84 -0.3 131,013 + ybgF COO64_RS20135 0.84 +0.4 131,013 + ybgF COO64_RS20135 0.84 +0.4 131,014 - ybgF COO64_RS20135 0.84 +0.5 131,029 + ybgF COO64_RS20135 0.86 +0.1 131,029 + ybgF COO64_RS20135 0.86 +0.1 131,029 + ybgF COO64_RS20135 0.86 -0.1 131,029 + ybgF COO64_RS20135 0.86 -1.0 131,029 + ybgF COO64_RS20135 0.86 -0.5 131,029 + ybgF COO64_RS20135 0.86 +2.0 131,030 - ybgF COO64_RS20135 0.86 +0.8 131,030 - ybgF COO64_RS20135 0.86 +0.4 131,030 - ybgF COO64_RS20135 0.86 -0.7 131,030 - ybgF COO64_RS20135 0.86 +0.8 131,209 - +0.2 131,230 + -0.7 131,280 + +0.2 131,280 + +0.4 131,280 + +0.7 131,281 - -0.0 131,281 - -1.3 131,281 - -0.7 131,302 + +0.2 131,302 + -1.5 131,316 + -1.0 131,316 + -0.4 131,316 + +0.1 131,316 + -0.4 131,316 + +0.5 131,316 + +0.2 131,316 + -0.2 131,316 + +0.5 131,317 - -0.9 131,317 - -1.0 131,317 - -1.0 131,317 - +0.3 131,317 - +1.4 131,317 - -1.8 131,317 - +1.0 131,317 - -0.5 131,317 - +0.3 131,317 - -1.3 131,317 - -0.7 131,317 - -1.3 131,320 + -0.2 131,320 + +0.4 131,320 + +0.9 131,320 + -0.1 131,321 - -0.3 131,321 - +0.5 131,417 + queE COO64_RS20140 0.18 -0.9 131,432 + queE COO64_RS20140 0.20 +0.5 131,432 + queE COO64_RS20140 0.20 +0.5 131,433 - queE COO64_RS20140 0.20 +0.7 131,433 - queE COO64_RS20140 0.20 -2.0 131,493 - queE COO64_RS20140 0.29 +0.1 131,493 - queE COO64_RS20140 0.29 -0.0 131,493 - queE COO64_RS20140 0.29 +0.9 131,504 + queE COO64_RS20140 0.31 -1.3 131,583 - queE COO64_RS20140 0.43 +0.6 131,618 - queE COO64_RS20140 0.49 -0.3 131,618 - queE COO64_RS20140 0.49 +0.1 131,743 + queE COO64_RS20140 0.68 +0.3 131,744 - queE COO64_RS20140 0.68 -0.0 131,762 + queE COO64_RS20140 0.71 -0.3 131,762 + queE COO64_RS20140 0.71 -0.6 131,762 + queE COO64_RS20140 0.71 -1.9 131,763 - queE COO64_RS20140 0.71 -1.7 131,763 - queE COO64_RS20140 0.71 +0.0 131,983 + -0.0 132,046 - queC COO64_RS20145 0.12 +1.2 132,046 - queC COO64_RS20145 0.12 -0.9 132,046 - queC COO64_RS20145 0.12 +0.5 132,047 + queC COO64_RS20145 0.12 -0.0 132,047 + queC COO64_RS20145 0.12 -0.2 132,047 + queC COO64_RS20145 0.12 -0.9 132,047 + queC COO64_RS20145 0.12 -0.5 132,047 + queC COO64_RS20145 0.12 -0.6 132,047 + queC COO64_RS20145 0.12 +0.5 132,048 - queC COO64_RS20145 0.12 -2.4 132,048 - queC COO64_RS20145 0.12 -0.3 132,048 - queC COO64_RS20145 0.12 +0.1 132,054 + queC COO64_RS20145 0.13 -0.5 132,054 + queC COO64_RS20145 0.13 +0.3 132,055 - queC COO64_RS20145 0.13 +1.0 132,055 - queC COO64_RS20145 0.13 +0.3 132,055 - queC COO64_RS20145 0.13 -0.8 132,055 - queC COO64_RS20145 0.13 +1.2 132,055 - queC COO64_RS20145 0.13 -0.4 132,055 - queC COO64_RS20145 0.13 -0.3 132,055 - queC COO64_RS20145 0.13 +0.5 132,170 + queC COO64_RS20145 0.31 -0.2 132,209 - queC COO64_RS20145 0.36 +0.7 132,243 + queC COO64_RS20145 0.41 -0.3 132,243 + queC COO64_RS20145 0.41 -0.3 132,243 + queC COO64_RS20145 0.41 +1.5 132,243 + queC COO64_RS20145 0.41 -0.2 132,243 + queC COO64_RS20145 0.41 +0.3 132,243 + queC COO64_RS20145 0.41 -0.3 132,243 + queC COO64_RS20145 0.41 +0.2 132,243 + queC COO64_RS20145 0.41 -0.8 132,244 - queC COO64_RS20145 0.41 -2.2 132,244 - queC COO64_RS20145 0.41 +1.1 132,244 - queC COO64_RS20145 0.41 -1.0 132,260 + queC COO64_RS20145 0.44 +2.1 132,260 + queC COO64_RS20145 0.44 +0.8 132,260 + queC COO64_RS20145 0.44 -0.0 132,261 - queC COO64_RS20145 0.44 -0.5 132,261 - queC COO64_RS20145 0.44 -0.3 132,315 - queC COO64_RS20145 0.52 -0.6 132,342 + queC COO64_RS20145 0.56 +0.1 132,343 - queC COO64_RS20145 0.56 +0.5 132,510 + queC COO64_RS20145 0.81 -0.7 132,511 - queC COO64_RS20145 0.81 +0.1 132,531 + queC COO64_RS20145 0.84 +0.1 132,613 + +0.2 132,749 + COO64_RS20150 0.64 +1.2 132,749 + COO64_RS20150 0.64 -0.9 132,749 + COO64_RS20150 0.64 -0.2 132,776 + -0.5 132,776 + -0.6 132,776 + -0.6 132,783 + +0.2 132,783 + +0.4 132,860 - -0.9 132,870 + -0.5 132,870 + -0.8 132,870 + -1.6 132,870 + -0.5 132,870 + +0.7 132,870 + +0.2 132,870 + +0.5 132,870 + -0.0 132,870 + -1.0 132,871 - -0.5 132,871 - +0.4 132,871 - +0.6 132,871 - -0.7 132,871 - +0.5 132,871 - -0.8 132,871 - +1.0 132,871 - -0.8 132,871 - +0.3 132,904 + -0.5 132,904 + -0.2 132,904 + -1.2 132,905 - -0.5 132,905 - +0.7 132,905 - +0.3 132,905 - -0.0 132,920 + +0.2 132,920 + -0.9 132,920 + -0.1 132,921 - +0.5 132,921 - -0.8 132,921 - +1.7
Or see this region's nucleotide sequence