Strain Fitness in Escherichia coli ECRC101 around MCAODC_22875

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagZ and thiK are separated by 10 nucleotidesthiK and lpoB overlap by 20 nucleotideslpoB and MCAODC_22880 are separated by 13 nucleotidesMCAODC_22880 and hinT are separated by 2 nucleotides MCAODC_22865: nagZ - beta-N-acetylhexosaminidase, at 1,316,252 to 1,317,277 nagZ MCAODC_22870: thiK - thiamine kinase, at 1,317,288 to 1,318,112 thiK MCAODC_22875: lpoB - penicillin-binding protein activator LpoB, at 1,318,093 to 1,318,734 lpoB MCAODC_22880: MCAODC_22880 - DUF1425 domain-containing protein, at 1,318,748 to 1,319,125 _22880 MCAODC_22885: hinT - purine nucleoside phosphoramidase, at 1,319,128 to 1,319,487 hinT Position (kb) 1318 1319Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1317.219 kb on - strandat 1317.290 kb on - strandat 1317.304 kb on - strandat 1317.304 kb on - strandat 1317.304 kb on - strandat 1317.336 kb on - strandat 1317.368 kb on - strandat 1317.430 kb on - strand, within thiKat 1317.611 kb on + strand, within thiKat 1317.612 kb on - strand, within thiKat 1317.612 kb on - strand, within thiKat 1317.625 kb on - strand, within thiKat 1317.625 kb on - strand, within thiKat 1317.778 kb on + strand, within thiKat 1317.782 kb on - strand, within thiKat 1317.868 kb on + strand, within thiKat 1317.869 kb on - strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.871 kb on - strand, within thiKat 1317.871 kb on - strand, within thiKat 1318.025 kb on + strand, within thiKat 1318.026 kb on - strand, within thiKat 1318.029 kb on - strand, within thiKat 1318.095 kb on - strandat 1318.095 kb on - strandat 1318.116 kb on + strandat 1318.117 kb on - strandat 1318.203 kb on - strand, within lpoBat 1318.211 kb on + strand, within lpoBat 1318.252 kb on - strand, within lpoBat 1318.285 kb on + strand, within lpoBat 1318.286 kb on - strand, within lpoBat 1318.286 kb on - strand, within lpoBat 1318.286 kb on - strand, within lpoBat 1318.467 kb on + strand, within lpoBat 1318.467 kb on + strand, within lpoBat 1318.468 kb on - strand, within lpoBat 1318.932 kb on + strand, within MCAODC_22880at 1318.932 kb on + strand, within MCAODC_22880at 1318.933 kb on - strand, within MCAODC_22880at 1319.084 kb on + strand, within MCAODC_22880at 1319.085 kb on - strand, within MCAODC_22880at 1319.085 kb on - strand, within MCAODC_22880at 1319.129 kb on + strandat 1319.196 kb on - strand, within hinTat 1319.508 kb on - strandat 1319.528 kb on + strandat 1319.552 kb on - strandat 1319.552 kb on - strandat 1319.557 kb on - strandat 1319.627 kb on + strandat 1319.627 kb on + strandat 1319.629 kb on + strandat 1319.629 kb on + strandat 1319.629 kb on + strandat 1319.630 kb on - strandat 1319.630 kb on - strandat 1319.631 kb on + strandat 1319.632 kb on - strandat 1319.632 kb on - strandat 1319.632 kb on - strandat 1319.632 kb on - strandat 1319.632 kb on - strandat 1319.632 kb on - strandat 1319.641 kb on - strandat 1319.691 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,317,219 - -0.0
1,317,290 - +1.1
1,317,304 - +0.7
1,317,304 - +0.1
1,317,304 - -0.6
1,317,336 - -0.7
1,317,368 - -0.5
1,317,430 - thiK MCAODC_22870 0.17 +0.3
1,317,611 + thiK MCAODC_22870 0.39 +0.1
1,317,612 - thiK MCAODC_22870 0.39 +0.5
1,317,612 - thiK MCAODC_22870 0.39 -2.2
1,317,625 - thiK MCAODC_22870 0.41 +0.1
1,317,625 - thiK MCAODC_22870 0.41 +0.4
1,317,778 + thiK MCAODC_22870 0.59 +0.1
1,317,782 - thiK MCAODC_22870 0.60 +1.7
1,317,868 + thiK MCAODC_22870 0.70 +0.9
1,317,869 - thiK MCAODC_22870 0.70 +0.7
1,317,870 + thiK MCAODC_22870 0.71 +0.7
1,317,870 + thiK MCAODC_22870 0.71 +2.9
1,317,870 + thiK MCAODC_22870 0.71 -0.8
1,317,870 + thiK MCAODC_22870 0.71 -0.3
1,317,871 - thiK MCAODC_22870 0.71 -2.2
1,317,871 - thiK MCAODC_22870 0.71 -1.2
1,318,025 + thiK MCAODC_22870 0.89 +0.1
1,318,026 - thiK MCAODC_22870 0.89 +1.7
1,318,029 - thiK MCAODC_22870 0.90 +0.1
1,318,095 - +0.5
1,318,095 - -0.2
1,318,116 + +1.2
1,318,117 - +1.0
1,318,203 - lpoB MCAODC_22875 0.17 +0.7
1,318,211 + lpoB MCAODC_22875 0.18 +0.3
1,318,252 - lpoB MCAODC_22875 0.25 +1.7
1,318,285 + lpoB MCAODC_22875 0.30 +0.1
1,318,286 - lpoB MCAODC_22875 0.30 -1.9
1,318,286 - lpoB MCAODC_22875 0.30 +2.7
1,318,286 - lpoB MCAODC_22875 0.30 -0.7
1,318,467 + lpoB MCAODC_22875 0.58 -0.2
1,318,467 + lpoB MCAODC_22875 0.58 +0.4
1,318,468 - lpoB MCAODC_22875 0.58 -0.7
1,318,932 + MCAODC_22880 0.49 -0.2
1,318,932 + MCAODC_22880 0.49 -0.9
1,318,933 - MCAODC_22880 0.49 -0.0
1,319,084 + MCAODC_22880 0.89 -0.3
1,319,085 - MCAODC_22880 0.89 +1.1
1,319,085 - MCAODC_22880 0.89 +1.7
1,319,129 + -1.3
1,319,196 - hinT MCAODC_22885 0.19 -1.2
1,319,508 - +0.3
1,319,528 + -2.7
1,319,552 - +1.1
1,319,552 - +2.7
1,319,557 - -0.2
1,319,627 + -1.7
1,319,627 + +0.7
1,319,629 + +0.2
1,319,629 + +0.1
1,319,629 + +1.7
1,319,630 - +1.3
1,319,630 - +0.4
1,319,631 + +0.6
1,319,632 - +0.8
1,319,632 - -0.7
1,319,632 - +0.7
1,319,632 - +0.5
1,319,632 - -0.2
1,319,632 - +1.1
1,319,641 - -0.7
1,319,691 + -1.5

Or see this region's nucleotide sequence