Strain Fitness in Escherichia coli ECRC101 around MCAODC_22860

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntndh and ycfP are separated by 399 nucleotidesycfP and nagZ are separated by 22 nucleotides MCAODC_22855: ndh - NADH-quinone dehydrogenase, at 1,313,983 to 1,315,287 ndh MCAODC_22860: ycfP - alpha/beta hydrolase YcfP, at 1,315,687 to 1,316,229 ycfP MCAODC_22865: nagZ - beta-N-acetylhexosaminidase, at 1,316,252 to 1,317,277 nagZ Position (kb) 1315 1316 1317Strain fitness (log2 ratio) -1 0 1 2 3at 1314.945 kb on - strand, within ndhat 1315.030 kb on - strand, within ndhat 1315.210 kb on - strandat 1315.282 kb on - strandat 1315.282 kb on - strandat 1315.282 kb on - strandat 1315.338 kb on + strandat 1315.339 kb on - strandat 1315.530 kb on + strandat 1315.530 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.544 kb on + strandat 1315.667 kb on - strandat 1315.710 kb on + strandat 1315.711 kb on - strandat 1315.735 kb on - strandat 1315.831 kb on - strand, within ycfPat 1315.890 kb on + strand, within ycfPat 1315.891 kb on - strand, within ycfPat 1315.891 kb on - strand, within ycfPat 1315.907 kb on + strand, within ycfPat 1315.959 kb on + strand, within ycfPat 1315.960 kb on - strand, within ycfPat 1316.104 kb on - strand, within ycfPat 1316.104 kb on - strand, within ycfPat 1316.104 kb on - strand, within ycfPat 1316.135 kb on - strand, within ycfPat 1316.144 kb on - strand, within ycfPat 1316.171 kb on - strand, within ycfPat 1316.219 kb on + strandat 1316.223 kb on + strandat 1316.223 kb on + strandat 1316.224 kb on - strandat 1316.242 kb on + strandat 1316.242 kb on + strandat 1316.288 kb on + strandat 1316.407 kb on + strand, within nagZat 1316.432 kb on - strand, within nagZat 1316.497 kb on - strand, within nagZat 1316.632 kb on - strand, within nagZat 1316.698 kb on + strand, within nagZat 1316.765 kb on + strand, within nagZat 1316.874 kb on + strand, within nagZat 1316.902 kb on + strand, within nagZat 1316.903 kb on - strand, within nagZat 1316.910 kb on - strand, within nagZat 1316.942 kb on - strand, within nagZat 1317.219 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,314,945 - ndh MCAODC_22855 0.74 -0.7
1,315,030 - ndh MCAODC_22855 0.80 +0.9
1,315,210 - -0.5
1,315,282 - -0.9
1,315,282 - +0.1
1,315,282 - -0.1
1,315,338 + +0.4
1,315,339 - +1.5
1,315,530 + -1.5
1,315,530 + +3.7
1,315,544 + -0.7
1,315,544 + -0.3
1,315,544 + -0.9
1,315,544 + -0.3
1,315,544 + +1.2
1,315,544 + +2.2
1,315,544 + +0.7
1,315,544 + -1.0
1,315,667 - -1.1
1,315,710 + +0.5
1,315,711 - -0.5
1,315,735 - -0.7
1,315,831 - ycfP MCAODC_22860 0.27 -0.5
1,315,890 + ycfP MCAODC_22860 0.37 -0.8
1,315,891 - ycfP MCAODC_22860 0.38 +0.7
1,315,891 - ycfP MCAODC_22860 0.38 -1.3
1,315,907 + ycfP MCAODC_22860 0.41 -0.9
1,315,959 + ycfP MCAODC_22860 0.50 +1.1
1,315,960 - ycfP MCAODC_22860 0.50 -1.2
1,316,104 - ycfP MCAODC_22860 0.77 +1.7
1,316,104 - ycfP MCAODC_22860 0.77 -0.9
1,316,104 - ycfP MCAODC_22860 0.77 -1.4
1,316,135 - ycfP MCAODC_22860 0.83 +1.7
1,316,144 - ycfP MCAODC_22860 0.84 +0.7
1,316,171 - ycfP MCAODC_22860 0.89 +0.7
1,316,219 + +0.2
1,316,223 + +1.1
1,316,223 + -0.4
1,316,224 - -0.3
1,316,242 + -0.3
1,316,242 + +0.1
1,316,288 + -1.1
1,316,407 + nagZ MCAODC_22865 0.15 +0.5
1,316,432 - nagZ MCAODC_22865 0.18 -1.2
1,316,497 - nagZ MCAODC_22865 0.24 -0.2
1,316,632 - nagZ MCAODC_22865 0.37 +1.3
1,316,698 + nagZ MCAODC_22865 0.43 +0.2
1,316,765 + nagZ MCAODC_22865 0.50 +1.2
1,316,874 + nagZ MCAODC_22865 0.61 +0.2
1,316,902 + nagZ MCAODC_22865 0.63 +0.9
1,316,903 - nagZ MCAODC_22865 0.63 -0.9
1,316,910 - nagZ MCAODC_22865 0.64 +0.3
1,316,942 - nagZ MCAODC_22865 0.67 +1.3
1,317,219 - -0.0

Or see this region's nucleotide sequence