Strain Fitness in Escherichia coli ECRC101 around MCAODC_22465

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpotA and potB overlap by 17 nucleotidespotB and espK are separated by 363 nucleotides MCAODC_22460: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 1,251,290 to 1,252,426 potA MCAODC_22465: potB - spermidine/putrescine ABC transporter permease PotB, at 1,252,410 to 1,253,273 potB MCAODC_22470: espK - type III secretion system effector EspK, at 1,253,637 to 1,254,998 espK Position (kb) 1252 1253 1254Strain fitness (log2 ratio) -2 -1 0 1 2at 1251.581 kb on + strand, within potAat 1251.581 kb on + strand, within potAat 1251.886 kb on + strand, within potAat 1251.887 kb on - strand, within potAat 1252.045 kb on + strand, within potAat 1252.117 kb on + strand, within potAat 1252.117 kb on + strand, within potAat 1252.120 kb on + strand, within potAat 1252.120 kb on + strand, within potAat 1252.124 kb on + strand, within potAat 1252.125 kb on - strand, within potAat 1252.125 kb on - strand, within potAat 1252.185 kb on + strand, within potAat 1252.186 kb on - strand, within potAat 1252.235 kb on + strand, within potAat 1252.250 kb on - strand, within potAat 1252.254 kb on - strand, within potAat 1252.375 kb on + strandat 1252.424 kb on + strandat 1252.438 kb on + strandat 1252.566 kb on + strand, within potBat 1252.603 kb on + strand, within potBat 1252.941 kb on - strand, within potBat 1252.994 kb on + strand, within potBat 1253.076 kb on + strand, within potBat 1253.076 kb on + strand, within potBat 1253.077 kb on - strand, within potBat 1253.077 kb on - strand, within potBat 1253.077 kb on - strand, within potBat 1253.134 kb on + strand, within potBat 1253.134 kb on + strand, within potBat 1253.147 kb on + strand, within potBat 1253.184 kb on - strand, within potBat 1253.349 kb on - strandat 1253.450 kb on + strandat 1253.762 kb on - strandat 1253.822 kb on + strand, within espKat 1254.021 kb on + strand, within espKat 1254.022 kb on - strand, within espKat 1254.023 kb on + strand, within espKat 1254.024 kb on - strand, within espKat 1254.025 kb on + strand, within espKat 1254.129 kb on + strand, within espKat 1254.130 kb on - strand, within espKat 1254.130 kb on - strand, within espKat 1254.210 kb on - strand, within espKat 1254.212 kb on + strand, within espKat 1254.213 kb on - strand, within espKat 1254.215 kb on - strand, within espK

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,251,581 + potA MCAODC_22460 0.26 -0.4
1,251,581 + potA MCAODC_22460 0.26 -0.1
1,251,886 + potA MCAODC_22460 0.52 -2.4
1,251,887 - potA MCAODC_22460 0.53 -0.9
1,252,045 + potA MCAODC_22460 0.66 -0.5
1,252,117 + potA MCAODC_22460 0.73 -0.5
1,252,117 + potA MCAODC_22460 0.73 +0.6
1,252,120 + potA MCAODC_22460 0.73 -0.9
1,252,120 + potA MCAODC_22460 0.73 +0.6
1,252,124 + potA MCAODC_22460 0.73 -2.5
1,252,125 - potA MCAODC_22460 0.73 +0.3
1,252,125 - potA MCAODC_22460 0.73 +1.1
1,252,185 + potA MCAODC_22460 0.79 +0.4
1,252,186 - potA MCAODC_22460 0.79 +1.6
1,252,235 + potA MCAODC_22460 0.83 +2.0
1,252,250 - potA MCAODC_22460 0.84 +1.1
1,252,254 - potA MCAODC_22460 0.85 +1.0
1,252,375 + -0.4
1,252,424 + +1.6
1,252,438 + +1.6
1,252,566 + potB MCAODC_22465 0.18 -1.5
1,252,603 + potB MCAODC_22465 0.22 +1.6
1,252,941 - potB MCAODC_22465 0.61 +0.1
1,252,994 + potB MCAODC_22465 0.68 -0.8
1,253,076 + potB MCAODC_22465 0.77 -0.7
1,253,076 + potB MCAODC_22465 0.77 +1.6
1,253,077 - potB MCAODC_22465 0.77 -0.2
1,253,077 - potB MCAODC_22465 0.77 +1.6
1,253,077 - potB MCAODC_22465 0.77 +1.6
1,253,134 + potB MCAODC_22465 0.84 +0.1
1,253,134 + potB MCAODC_22465 0.84 -1.4
1,253,147 + potB MCAODC_22465 0.85 +2.1
1,253,184 - potB MCAODC_22465 0.90 +1.1
1,253,349 - -2.4
1,253,450 + +0.1
1,253,762 - +0.1
1,253,822 + espK MCAODC_22470 0.14 +1.6
1,254,021 + espK MCAODC_22470 0.28 +1.6
1,254,022 - espK MCAODC_22470 0.28 -0.4
1,254,023 + espK MCAODC_22470 0.28 -0.7
1,254,024 - espK MCAODC_22470 0.28 -1.4
1,254,025 + espK MCAODC_22470 0.28 +0.1
1,254,129 + espK MCAODC_22470 0.36 +0.6
1,254,130 - espK MCAODC_22470 0.36 +0.6
1,254,130 - espK MCAODC_22470 0.36 -0.9
1,254,210 - espK MCAODC_22470 0.42 +1.1
1,254,212 + espK MCAODC_22470 0.42 -0.7
1,254,213 - espK MCAODC_22470 0.42 -2.4
1,254,215 - espK MCAODC_22470 0.42 +0.1

Or see this region's nucleotide sequence