Strain Fitness in Escherichia coli ECRC101 around MCAODC_20230

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeO and Asn_trna are separated by 193 nucleotidesAsn_trna and cbl are separated by 37 nucleotides MCAODC_20225: yeeO - toxic metabolite efflux MATE transporter YeeO, at 856,878 to 858,332 yeeO MCAODC_20230: Asn_trna - tRNA-Asn(gtt), at 858,526 to 858,601 _trna MCAODC_20235: cbl - HTH-type transcriptional regulator Cbl, at 858,639 to 859,589 cbl Position (kb) 858 859Strain fitness (log2 ratio) -2 -1 0 1 2 3at 857.747 kb on + strand, within yeeOat 857.748 kb on - strand, within yeeOat 857.753 kb on + strand, within yeeOat 857.753 kb on + strand, within yeeOat 857.898 kb on - strand, within yeeOat 857.957 kb on + strand, within yeeOat 858.069 kb on - strand, within yeeOat 858.072 kb on - strand, within yeeOat 858.241 kb on - strandat 858.265 kb on + strandat 858.265 kb on + strandat 858.342 kb on + strandat 858.444 kb on + strandat 858.444 kb on + strandat 858.743 kb on - strand, within cblat 858.747 kb on - strand, within cblat 858.785 kb on + strand, within cblat 858.785 kb on + strand, within cblat 858.786 kb on - strand, within cblat 858.788 kb on + strand, within cblat 858.789 kb on - strand, within cblat 858.789 kb on - strand, within cblat 858.865 kb on - strand, within cblat 858.865 kb on - strand, within cblat 858.892 kb on + strand, within cblat 858.893 kb on - strand, within cblat 858.893 kb on - strand, within cblat 858.900 kb on - strand, within cblat 858.924 kb on + strand, within cblat 858.924 kb on + strand, within cblat 858.927 kb on + strand, within cblat 858.928 kb on - strand, within cblat 858.928 kb on - strand, within cblat 858.929 kb on + strand, within cblat 858.929 kb on + strand, within cblat 858.932 kb on + strand, within cblat 858.933 kb on - strand, within cblat 858.971 kb on + strand, within cblat 858.972 kb on - strand, within cblat 858.986 kb on + strand, within cblat 858.986 kb on + strand, within cblat 859.047 kb on - strand, within cblat 859.085 kb on + strand, within cblat 859.086 kb on - strand, within cblat 859.271 kb on + strand, within cblat 859.271 kb on + strand, within cblat 859.271 kb on + strand, within cblat 859.271 kb on + strand, within cblat 859.272 kb on - strand, within cblat 859.272 kb on - strand, within cblat 859.274 kb on - strand, within cblat 859.286 kb on + strand, within cblat 859.287 kb on - strand, within cblat 859.319 kb on + strand, within cblat 859.373 kb on + strand, within cblat 859.374 kb on - strand, within cblat 859.392 kb on + strand, within cblat 859.518 kb on - strandat 859.541 kb on - strandat 859.567 kb on - strandat 859.572 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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857,747 + yeeO MCAODC_20225 0.60 +2.6
857,748 - yeeO MCAODC_20225 0.60 -1.0
857,753 + yeeO MCAODC_20225 0.60 +0.4
857,753 + yeeO MCAODC_20225 0.60 -0.4
857,898 - yeeO MCAODC_20225 0.70 -2.1
857,957 + yeeO MCAODC_20225 0.74 -1.6
858,069 - yeeO MCAODC_20225 0.82 -1.4
858,072 - yeeO MCAODC_20225 0.82 -2.1
858,241 - +0.4
858,265 + -0.5
858,265 + +1.4
858,342 + -0.8
858,444 + +0.6
858,444 + +2.9
858,743 - cbl MCAODC_20235 0.11 +0.6
858,747 - cbl MCAODC_20235 0.11 -0.4
858,785 + cbl MCAODC_20235 0.15 +0.9
858,785 + cbl MCAODC_20235 0.15 -0.0
858,786 - cbl MCAODC_20235 0.15 -1.1
858,788 + cbl MCAODC_20235 0.16 +0.0
858,789 - cbl MCAODC_20235 0.16 +0.0
858,789 - cbl MCAODC_20235 0.16 +3.2
858,865 - cbl MCAODC_20235 0.24 -0.4
858,865 - cbl MCAODC_20235 0.24 -1.0
858,892 + cbl MCAODC_20235 0.27 +0.9
858,893 - cbl MCAODC_20235 0.27 +1.0
858,893 - cbl MCAODC_20235 0.27 +1.8
858,900 - cbl MCAODC_20235 0.27 +1.6
858,924 + cbl MCAODC_20235 0.30 -1.4
858,924 + cbl MCAODC_20235 0.30 +2.0
858,927 + cbl MCAODC_20235 0.30 -2.1
858,928 - cbl MCAODC_20235 0.30 -0.5
858,928 - cbl MCAODC_20235 0.30 +1.0
858,929 + cbl MCAODC_20235 0.30 -1.1
858,929 + cbl MCAODC_20235 0.30 +1.3
858,932 + cbl MCAODC_20235 0.31 +0.9
858,933 - cbl MCAODC_20235 0.31 +0.6
858,971 + cbl MCAODC_20235 0.35 +0.7
858,972 - cbl MCAODC_20235 0.35 -0.7
858,986 + cbl MCAODC_20235 0.36 +0.9
858,986 + cbl MCAODC_20235 0.36 +0.6
859,047 - cbl MCAODC_20235 0.43 +0.0
859,085 + cbl MCAODC_20235 0.47 +0.4
859,086 - cbl MCAODC_20235 0.47 +0.6
859,271 + cbl MCAODC_20235 0.66 +0.9
859,271 + cbl MCAODC_20235 0.66 -1.3
859,271 + cbl MCAODC_20235 0.66 -1.0
859,271 + cbl MCAODC_20235 0.66 +1.4
859,272 - cbl MCAODC_20235 0.67 -0.1
859,272 - cbl MCAODC_20235 0.67 -1.4
859,274 - cbl MCAODC_20235 0.67 +0.0
859,286 + cbl MCAODC_20235 0.68 +0.6
859,287 - cbl MCAODC_20235 0.68 +0.5
859,319 + cbl MCAODC_20235 0.72 -0.3
859,373 + cbl MCAODC_20235 0.77 +1.6
859,374 - cbl MCAODC_20235 0.77 -0.8
859,392 + cbl MCAODC_20235 0.79 -2.0
859,518 - -0.2
859,541 - -2.1
859,567 - +0.6
859,572 + +1.2

Or see this region's nucleotide sequence