Strain Fitness in Escherichia coli ECRC101 around MCAODC_18295

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiV and nlpC are separated by 246 nucleotidesnlpC and btuD are separated by 77 nucleotidesbtuD and btuE overlap by 1 nucleotides MCAODC_18290: ydiV - anti-FlhDC factor, at 520,289 to 521,002 ydiV MCAODC_18295: nlpC - putative endopeptidase NlpC, at 521,249 to 521,713 nlpC MCAODC_18300: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 521,791 to 522,540 btuD MCAODC_18305: btuE - bifunctional thioredoxin/glutathione peroxidase, at 522,540 to 523,091 btuE Position (kb) 521 522Strain fitness (log2 ratio) -1 0 1at 520.249 kb on - strandat 520.268 kb on - strandat 521.061 kb on - strandat 521.150 kb on - strandat 521.192 kb on - strandat 521.196 kb on + strandat 521.287 kb on - strandat 521.338 kb on + strand, within nlpCat 521.589 kb on - strand, within nlpCat 521.924 kb on - strand, within btuDat 522.224 kb on - strand, within btuDat 522.520 kb on + strandat 522.521 kb on - strandat 522.561 kb on - strandat 522.597 kb on - strand, within btuE

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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520,249 - -0.7
520,268 - +0.1
521,061 - +0.6
521,150 - -0.9
521,192 - +0.7
521,196 + +0.9
521,287 - -0.2
521,338 + nlpC MCAODC_18295 0.19 +0.4
521,589 - nlpC MCAODC_18295 0.73 -0.9
521,924 - btuD MCAODC_18300 0.18 -1.4
522,224 - btuD MCAODC_18300 0.58 -0.4
522,520 + -1.3
522,521 - +0.9
522,561 - +0.6
522,597 - btuE MCAODC_18305 0.10 +1.6

Or see this region's nucleotide sequence