Strain Fitness in Escherichia coli ECRC101 around MCAODC_18055

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmdtK and ydhQ are separated by 40 nucleotidesydhQ and Val_trna are separated by 307 nucleotidesVal_trna and Val_trna are separated by 4 nucleotidesVal_trna and ydhR are separated by 107 nucleotides MCAODC_18050: mdtK - multidrug efflux MATE transporter MdtK, at 472,437 to 473,810 mdtK MCAODC_18055: ydhQ - Uncharacterized protein YdhQ, at 473,851 to 475,107 ydhQ MCAODC_18060: Val_trna - tRNA-Val(gac), at 475,415 to 475,491 _trna MCAODC_18065: Val_trna - tRNA-Val(gac), at 475,496 to 475,572 _trna MCAODC_18075: ydhR - monooxygenase, at 475,680 to 475,985 ydhR Position (kb) 473 474 475 476Strain fitness (log2 ratio) -2 -1 0 1 2 3at 472.962 kb on + strand, within mdtKat 472.962 kb on + strand, within mdtKat 472.962 kb on + strand, within mdtKat 472.962 kb on + strand, within mdtKat 472.963 kb on - strand, within mdtKat 472.963 kb on - strand, within mdtKat 472.963 kb on - strand, within mdtKat 473.037 kb on + strand, within mdtKat 473.037 kb on + strand, within mdtKat 473.067 kb on + strand, within mdtKat 473.067 kb on + strand, within mdtKat 473.221 kb on + strand, within mdtKat 473.394 kb on - strand, within mdtKat 473.466 kb on + strand, within mdtKat 473.578 kb on - strand, within mdtKat 473.601 kb on + strand, within mdtKat 473.647 kb on - strand, within mdtKat 473.682 kb on - strandat 473.711 kb on + strandat 473.738 kb on + strandat 474.250 kb on + strand, within ydhQat 474.353 kb on - strand, within ydhQat 474.353 kb on - strand, within ydhQat 474.353 kb on - strand, within ydhQat 474.406 kb on - strand, within ydhQat 474.438 kb on - strand, within ydhQat 474.468 kb on - strand, within ydhQat 474.489 kb on + strand, within ydhQat 474.490 kb on - strand, within ydhQat 474.543 kb on + strand, within ydhQat 474.544 kb on - strand, within ydhQat 474.544 kb on - strand, within ydhQat 474.544 kb on - strand, within ydhQat 474.644 kb on - strand, within ydhQat 474.644 kb on - strand, within ydhQat 474.770 kb on + strand, within ydhQat 474.770 kb on + strand, within ydhQat 474.771 kb on - strand, within ydhQat 474.771 kb on - strand, within ydhQat 474.829 kb on - strand, within ydhQat 474.837 kb on + strand, within ydhQat 474.915 kb on - strand, within ydhQat 474.915 kb on - strand, within ydhQat 475.024 kb on - strandat 475.024 kb on - strandat 475.139 kb on + strandat 475.145 kb on + strandat 475.146 kb on - strandat 475.146 kb on - strandat 475.149 kb on + strandat 475.334 kb on + strandat 475.406 kb on + strandat 475.406 kb on + strandat 475.422 kb on + strandat 475.641 kb on + strandat 475.641 kb on + strandat 475.748 kb on + strand, within ydhRat 476.106 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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472,962 + mdtK MCAODC_18050 0.38 -1.2
472,962 + mdtK MCAODC_18050 0.38 -0.5
472,962 + mdtK MCAODC_18050 0.38 +0.1
472,962 + mdtK MCAODC_18050 0.38 -0.9
472,963 - mdtK MCAODC_18050 0.38 -0.3
472,963 - mdtK MCAODC_18050 0.38 -0.4
472,963 - mdtK MCAODC_18050 0.38 -1.8
473,037 + mdtK MCAODC_18050 0.44 +1.1
473,037 + mdtK MCAODC_18050 0.44 -0.9
473,067 + mdtK MCAODC_18050 0.46 -0.2
473,067 + mdtK MCAODC_18050 0.46 +0.2
473,221 + mdtK MCAODC_18050 0.57 +0.6
473,394 - mdtK MCAODC_18050 0.70 +1.6
473,466 + mdtK MCAODC_18050 0.75 -0.5
473,578 - mdtK MCAODC_18050 0.83 -0.9
473,601 + mdtK MCAODC_18050 0.85 +0.1
473,647 - mdtK MCAODC_18050 0.88 -0.4
473,682 - +0.5
473,711 + +0.1
473,738 + -0.9
474,250 + ydhQ MCAODC_18055 0.32 +1.6
474,353 - ydhQ MCAODC_18055 0.40 -0.7
474,353 - ydhQ MCAODC_18055 0.40 -0.9
474,353 - ydhQ MCAODC_18055 0.40 +0.3
474,406 - ydhQ MCAODC_18055 0.44 -1.1
474,438 - ydhQ MCAODC_18055 0.47 -1.7
474,468 - ydhQ MCAODC_18055 0.49 +1.1
474,489 + ydhQ MCAODC_18055 0.51 -0.7
474,490 - ydhQ MCAODC_18055 0.51 -1.4
474,543 + ydhQ MCAODC_18055 0.55 +0.6
474,544 - ydhQ MCAODC_18055 0.55 +1.1
474,544 - ydhQ MCAODC_18055 0.55 -1.5
474,544 - ydhQ MCAODC_18055 0.55 +1.4
474,644 - ydhQ MCAODC_18055 0.63 +1.6
474,644 - ydhQ MCAODC_18055 0.63 +0.3
474,770 + ydhQ MCAODC_18055 0.73 +0.3
474,770 + ydhQ MCAODC_18055 0.73 -0.5
474,771 - ydhQ MCAODC_18055 0.73 +0.1
474,771 - ydhQ MCAODC_18055 0.73 +1.6
474,829 - ydhQ MCAODC_18055 0.78 +0.4
474,837 + ydhQ MCAODC_18055 0.78 +0.3
474,915 - ydhQ MCAODC_18055 0.85 -0.4
474,915 - ydhQ MCAODC_18055 0.85 -0.1
475,024 - +1.0
475,024 - +0.2
475,139 + +1.1
475,145 + +0.3
475,146 - -0.0
475,146 - +0.2
475,149 + +1.6
475,334 + +3.5
475,406 + +0.6
475,406 + +0.8
475,422 + +0.4
475,641 + +0.1
475,641 + +2.0
475,748 + ydhR MCAODC_18075 0.22 +1.6
476,106 + +0.1

Or see this region's nucleotide sequence