Strain Fitness in Escherichia coli ECRC101 around MCAODC_16805

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgGDEF and yneG are separated by 138 nucleotidesyneG and glsB overlap by 1 nucleotidesglsB and sad are separated by 63 nucleotides MCAODC_16800: gGDEF - diguanylate cyclase, at 272,393 to 273,811 gGDEF MCAODC_16805: yneG - Uncharacterized protein YneG, at 273,950 to 274,309 yneG MCAODC_16810: glsB - glutaminase B, at 274,309 to 275,235 glsB MCAODC_16815: sad - succinate-semialdehyde dehydrogenase, at 275,299 to 276,687 sad Position (kb) 273 274 275Strain fitness (log2 ratio) -1 0 1 2 3 4at 273.058 kb on + strand, within gGDEFat 273.212 kb on - strand, within gGDEFat 273.283 kb on + strand, within gGDEFat 273.284 kb on - strand, within gGDEFat 273.284 kb on - strand, within gGDEFat 273.359 kb on - strand, within gGDEFat 273.399 kb on - strand, within gGDEFat 273.402 kb on - strand, within gGDEFat 273.420 kb on + strand, within gGDEFat 273.424 kb on + strand, within gGDEFat 273.424 kb on + strand, within gGDEFat 273.444 kb on - strand, within gGDEFat 273.606 kb on + strand, within gGDEFat 273.606 kb on + strand, within gGDEFat 273.755 kb on - strandat 273.755 kb on - strandat 273.813 kb on - strandat 273.856 kb on - strandat 273.856 kb on - strandat 273.856 kb on - strandat 273.856 kb on - strandat 273.928 kb on - strandat 273.968 kb on + strandat 274.000 kb on + strand, within yneGat 274.002 kb on + strand, within yneGat 274.003 kb on - strand, within yneGat 274.045 kb on + strand, within yneGat 274.225 kb on - strand, within yneGat 274.510 kb on - strand, within glsBat 274.576 kb on - strand, within glsBat 274.786 kb on - strand, within glsBat 274.861 kb on - strand, within glsBat 274.934 kb on - strand, within glsBat 275.097 kb on + strand, within glsBat 275.103 kb on + strand, within glsBat 275.104 kb on - strand, within glsBat 275.164 kb on - strandat 275.276 kb on - strandat 275.291 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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273,058 + gGDEF MCAODC_16800 0.47 +4.6
273,212 - gGDEF MCAODC_16800 0.58 +1.6
273,283 + gGDEF MCAODC_16800 0.63 +0.2
273,284 - gGDEF MCAODC_16800 0.63 -0.8
273,284 - gGDEF MCAODC_16800 0.63 -1.2
273,359 - gGDEF MCAODC_16800 0.68 +0.1
273,399 - gGDEF MCAODC_16800 0.71 -1.3
273,402 - gGDEF MCAODC_16800 0.71 -0.4
273,420 + gGDEF MCAODC_16800 0.72 -0.4
273,424 + gGDEF MCAODC_16800 0.73 -0.5
273,424 + gGDEF MCAODC_16800 0.73 -0.0
273,444 - gGDEF MCAODC_16800 0.74 +1.6
273,606 + gGDEF MCAODC_16800 0.85 +0.5
273,606 + gGDEF MCAODC_16800 0.85 -0.4
273,755 - +1.8
273,755 - -0.9
273,813 - -0.4
273,856 - -0.6
273,856 - -0.2
273,856 - +0.1
273,856 - +0.6
273,928 - +0.6
273,968 + +0.6
274,000 + yneG MCAODC_16805 0.14 +0.2
274,002 + yneG MCAODC_16805 0.14 -0.9
274,003 - yneG MCAODC_16805 0.15 +0.1
274,045 + yneG MCAODC_16805 0.26 +0.6
274,225 - yneG MCAODC_16805 0.76 -0.7
274,510 - glsB MCAODC_16810 0.22 -0.4
274,576 - glsB MCAODC_16810 0.29 -0.4
274,786 - glsB MCAODC_16810 0.51 -0.7
274,861 - glsB MCAODC_16810 0.60 +0.6
274,934 - glsB MCAODC_16810 0.67 +0.6
275,097 + glsB MCAODC_16810 0.85 +3.2
275,103 + glsB MCAODC_16810 0.86 +0.6
275,104 - glsB MCAODC_16810 0.86 +2.6
275,164 - -0.8
275,276 - +0.3
275,291 - -0.6

Or see this region's nucleotide sequence