Strain Fitness in Escherichia coli ECRC101 around MCAODC_16170

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_16165 and pdxI are separated by 219 nucleotidespdxI and MCAODC_16175 are separated by 63 nucleotides MCAODC_16165: MCAODC_16165 - BigB-like protein, at 124,937 to 127,396 _16165 MCAODC_16170: pdxI - pyridoxine 4-dehydrogenase, at 127,616 to 128,476 pdxI MCAODC_16175: MCAODC_16175 - DUF2773 domain-containing protein, at 128,540 to 130,846 _16175 Position (kb) 127 128 129Strain fitness (log2 ratio) -1 0 1at 126.641 kb on + strand, within MCAODC_16165at 126.661 kb on + strand, within MCAODC_16165at 126.662 kb on - strand, within MCAODC_16165at 126.817 kb on + strand, within MCAODC_16165at 127.295 kb on + strandat 127.295 kb on + strandat 127.295 kb on + strandat 127.331 kb on + strandat 127.473 kb on - strandat 127.553 kb on - strandat 127.557 kb on - strandat 127.573 kb on - strandat 127.575 kb on - strandat 127.601 kb on - strandat 127.627 kb on + strandat 127.634 kb on - strandat 127.634 kb on - strandat 127.791 kb on - strand, within pdxIat 127.791 kb on - strand, within pdxIat 128.169 kb on - strand, within pdxIat 128.343 kb on + strand, within pdxIat 128.360 kb on - strand, within pdxIat 128.449 kb on + strandat 128.461 kb on + strandat 128.481 kb on + strandat 128.540 kb on - strandat 128.540 kb on - strandat 128.575 kb on - strandat 128.775 kb on + strand, within MCAODC_16175at 128.787 kb on + strand, within MCAODC_16175at 128.972 kb on + strand, within MCAODC_16175at 129.458 kb on + strand, within MCAODC_16175at 129.459 kb on - strand, within MCAODC_16175at 129.461 kb on - strand, within MCAODC_16175

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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126,641 + MCAODC_16165 0.69 +0.8
126,661 + MCAODC_16165 0.70 +0.3
126,662 - MCAODC_16165 0.70 +1.6
126,817 + MCAODC_16165 0.76 -0.7
127,295 + -0.2
127,295 + +0.6
127,295 + -0.3
127,331 + -1.4
127,473 - -0.7
127,553 - -1.5
127,557 - +0.9
127,573 - +0.6
127,575 - -0.9
127,601 - +1.3
127,627 + +0.4
127,634 - +0.3
127,634 - -0.6
127,791 - pdxI MCAODC_16170 0.20 +1.0
127,791 - pdxI MCAODC_16170 0.20 +0.9
128,169 - pdxI MCAODC_16170 0.64 +1.6
128,343 + pdxI MCAODC_16170 0.84 -1.6
128,360 - pdxI MCAODC_16170 0.86 +0.6
128,449 + -0.5
128,461 + +0.6
128,481 + -0.2
128,540 - -0.2
128,540 - -0.2
128,575 - +0.8
128,775 + MCAODC_16175 0.10 +0.6
128,787 + MCAODC_16175 0.11 -0.0
128,972 + MCAODC_16175 0.19 +0.6
129,458 + MCAODC_16175 0.40 +0.6
129,459 - MCAODC_16175 0.40 +0.5
129,461 - MCAODC_16175 0.40 +0.8

Or see this region's nucleotide sequence