Strain Fitness in Escherichia coli ECRC101 around MCAODC_11885

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgrcA and eamB are separated by 55 nucleotideseamB and yfiE are separated by 102 nucleotidesyfiE and srmB are separated by 32 nucleotides MCAODC_11875: grcA - autonomous glycyl radical cofactor GrcA, at 2,348,319 to 2,348,702 grcA MCAODC_11880: eamB - cysteine/O-acetylserine transporter, at 2,348,758 to 2,349,345 eamB MCAODC_11885: yfiE - Uncharacterized HTH-type transcriptional regulator YfiE, at 2,349,448 to 2,350,329 yfiE MCAODC_11890: srmB - ATP-dependent RNA helicase SrmB, at 2,350,362 to 2,351,696 srmB Position (kb) 2349 2350 2351Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2348.479 kb on - strand, within grcAat 2348.494 kb on + strand, within grcAat 2348.579 kb on + strand, within grcAat 2348.579 kb on + strand, within grcAat 2348.618 kb on - strand, within grcAat 2348.625 kb on + strand, within grcAat 2348.639 kb on + strand, within grcAat 2348.681 kb on + strandat 2348.682 kb on - strandat 2348.707 kb on + strandat 2348.708 kb on - strandat 2348.711 kb on + strandat 2348.711 kb on + strandat 2348.711 kb on + strandat 2348.711 kb on + strandat 2348.712 kb on - strandat 2348.712 kb on - strandat 2348.719 kb on + strandat 2348.719 kb on + strandat 2348.720 kb on - strandat 2348.720 kb on - strandat 2348.720 kb on - strandat 2348.762 kb on + strandat 2348.763 kb on - strandat 2348.783 kb on + strandat 2348.784 kb on - strandat 2348.813 kb on - strandat 2348.826 kb on - strand, within eamBat 2348.921 kb on - strand, within eamBat 2348.979 kb on - strand, within eamBat 2348.979 kb on - strand, within eamBat 2349.099 kb on + strand, within eamBat 2349.099 kb on + strand, within eamBat 2349.099 kb on + strand, within eamBat 2349.099 kb on + strand, within eamBat 2349.099 kb on + strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.100 kb on - strand, within eamBat 2349.101 kb on + strand, within eamBat 2349.101 kb on + strand, within eamBat 2349.101 kb on + strand, within eamBat 2349.101 kb on + strand, within eamBat 2349.102 kb on - strand, within eamBat 2349.102 kb on - strand, within eamBat 2349.102 kb on - strand, within eamBat 2349.261 kb on + strand, within eamBat 2349.273 kb on + strand, within eamBat 2349.274 kb on - strand, within eamBat 2349.274 kb on - strand, within eamBat 2349.311 kb on + strandat 2349.311 kb on + strandat 2349.328 kb on + strandat 2349.328 kb on + strandat 2349.329 kb on - strandat 2349.350 kb on + strandat 2349.350 kb on + strandat 2349.350 kb on + strandat 2349.388 kb on + strandat 2349.417 kb on + strandat 2349.447 kb on + strandat 2349.466 kb on - strandat 2349.469 kb on - strandat 2349.474 kb on + strandat 2349.552 kb on + strand, within yfiEat 2349.587 kb on + strand, within yfiEat 2349.587 kb on + strand, within yfiEat 2349.588 kb on - strand, within yfiEat 2349.588 kb on - strand, within yfiEat 2349.588 kb on - strand, within yfiEat 2349.652 kb on + strand, within yfiEat 2349.652 kb on + strand, within yfiEat 2349.652 kb on + strand, within yfiEat 2349.652 kb on + strand, within yfiEat 2349.652 kb on + strand, within yfiEat 2349.653 kb on - strand, within yfiEat 2349.676 kb on - strand, within yfiEat 2349.723 kb on - strand, within yfiEat 2349.723 kb on - strand, within yfiEat 2349.832 kb on + strand, within yfiEat 2349.996 kb on + strand, within yfiEat 2349.997 kb on - strand, within yfiEat 2350.034 kb on + strand, within yfiEat 2350.035 kb on - strand, within yfiEat 2350.117 kb on - strand, within yfiEat 2350.154 kb on - strand, within yfiEat 2350.154 kb on - strand, within yfiEat 2350.236 kb on + strand, within yfiEat 2350.293 kb on + strandat 2350.333 kb on + strandat 2350.364 kb on - strandat 2350.364 kb on - strandat 2350.364 kb on - strandat 2350.416 kb on - strandat 2350.504 kb on - strand, within srmBat 2350.573 kb on - strand, within srmBat 2350.583 kb on + strand, within srmBat 2350.583 kb on + strand, within srmBat 2350.584 kb on - strand, within srmBat 2350.584 kb on - strand, within srmBat 2350.642 kb on - strand, within srmBat 2350.642 kb on - strand, within srmBat 2350.702 kb on + strand, within srmBat 2350.702 kb on + strand, within srmBat 2350.929 kb on - strand, within srmBat 2350.994 kb on + strand, within srmBat 2350.995 kb on - strand, within srmBat 2351.021 kb on - strand, within srmBat 2351.177 kb on + strand, within srmBat 2351.178 kb on - strand, within srmBat 2351.178 kb on - strand, within srmBat 2351.274 kb on + strand, within srmBat 2351.275 kb on - strand, within srmBat 2351.278 kb on + strand, within srmBat 2351.278 kb on + strand, within srmBat 2351.279 kb on - strand, within srmBat 2351.279 kb on - strand, within srmBat 2351.279 kb on - strand, within srmB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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2,348,479 - grcA MCAODC_11875 0.42 -1.3
2,348,494 + grcA MCAODC_11875 0.46 +1.9
2,348,579 + grcA MCAODC_11875 0.68 +0.2
2,348,579 + grcA MCAODC_11875 0.68 -2.7
2,348,618 - grcA MCAODC_11875 0.78 -0.2
2,348,625 + grcA MCAODC_11875 0.80 -1.3
2,348,639 + grcA MCAODC_11875 0.83 +1.5
2,348,681 + -0.1
2,348,682 - -1.8
2,348,707 + +0.3
2,348,708 - +0.3
2,348,711 + +0.5
2,348,711 + +0.5
2,348,711 + -0.7
2,348,711 + -1.4
2,348,712 - -0.3
2,348,712 - +1.0
2,348,719 + +1.6
2,348,719 + -3.4
2,348,720 - +0.6
2,348,720 - -0.1
2,348,720 - -1.0
2,348,762 + -0.7
2,348,763 - -0.3
2,348,783 + +1.0
2,348,784 - -0.1
2,348,813 - -0.6
2,348,826 - eamB MCAODC_11880 0.12 +0.6
2,348,921 - eamB MCAODC_11880 0.28 -1.2
2,348,979 - eamB MCAODC_11880 0.38 -2.2
2,348,979 - eamB MCAODC_11880 0.38 -0.0
2,349,099 + eamB MCAODC_11880 0.58 -2.8
2,349,099 + eamB MCAODC_11880 0.58 -0.9
2,349,099 + eamB MCAODC_11880 0.58 -0.8
2,349,099 + eamB MCAODC_11880 0.58 +0.3
2,349,099 + eamB MCAODC_11880 0.58 -0.1
2,349,100 - eamB MCAODC_11880 0.58 -0.7
2,349,100 - eamB MCAODC_11880 0.58 +1.5
2,349,100 - eamB MCAODC_11880 0.58 +0.7
2,349,100 - eamB MCAODC_11880 0.58 -1.1
2,349,100 - eamB MCAODC_11880 0.58 -0.2
2,349,100 - eamB MCAODC_11880 0.58 -0.1
2,349,100 - eamB MCAODC_11880 0.58 +0.9
2,349,101 + eamB MCAODC_11880 0.58 -1.3
2,349,101 + eamB MCAODC_11880 0.58 -0.4
2,349,101 + eamB MCAODC_11880 0.58 +0.1
2,349,101 + eamB MCAODC_11880 0.58 +0.2
2,349,102 - eamB MCAODC_11880 0.59 -1.7
2,349,102 - eamB MCAODC_11880 0.59 -1.4
2,349,102 - eamB MCAODC_11880 0.59 +0.3
2,349,261 + eamB MCAODC_11880 0.86 +1.7
2,349,273 + eamB MCAODC_11880 0.88 +0.3
2,349,274 - eamB MCAODC_11880 0.88 +1.7
2,349,274 - eamB MCAODC_11880 0.88 +0.5
2,349,311 + -0.4
2,349,311 + -0.2
2,349,328 + +0.4
2,349,328 + +1.5
2,349,329 - +1.5
2,349,350 + +2.3
2,349,350 + -1.5
2,349,350 + +0.5
2,349,388 + +0.3
2,349,417 + -0.1
2,349,447 + +1.7
2,349,466 - +1.5
2,349,469 - +0.1
2,349,474 + -1.5
2,349,552 + yfiE MCAODC_11885 0.12 +0.8
2,349,587 + yfiE MCAODC_11885 0.16 +0.3
2,349,587 + yfiE MCAODC_11885 0.16 +0.9
2,349,588 - yfiE MCAODC_11885 0.16 +1.2
2,349,588 - yfiE MCAODC_11885 0.16 +0.1
2,349,588 - yfiE MCAODC_11885 0.16 -1.1
2,349,652 + yfiE MCAODC_11885 0.23 +1.0
2,349,652 + yfiE MCAODC_11885 0.23 +0.2
2,349,652 + yfiE MCAODC_11885 0.23 +0.1
2,349,652 + yfiE MCAODC_11885 0.23 -1.5
2,349,652 + yfiE MCAODC_11885 0.23 +0.9
2,349,653 - yfiE MCAODC_11885 0.23 +0.7
2,349,676 - yfiE MCAODC_11885 0.26 -0.9
2,349,723 - yfiE MCAODC_11885 0.31 +1.5
2,349,723 - yfiE MCAODC_11885 0.31 +0.5
2,349,832 + yfiE MCAODC_11885 0.44 +0.7
2,349,996 + yfiE MCAODC_11885 0.62 +0.7
2,349,997 - yfiE MCAODC_11885 0.62 +0.3
2,350,034 + yfiE MCAODC_11885 0.66 +0.7
2,350,035 - yfiE MCAODC_11885 0.67 +0.5
2,350,117 - yfiE MCAODC_11885 0.76 -0.1
2,350,154 - yfiE MCAODC_11885 0.80 -1.5
2,350,154 - yfiE MCAODC_11885 0.80 -1.3
2,350,236 + yfiE MCAODC_11885 0.89 -0.1
2,350,293 + +1.5
2,350,333 + -0.5
2,350,364 - -1.4
2,350,364 - +1.1
2,350,364 - +0.1
2,350,416 - +1.0
2,350,504 - srmB MCAODC_11890 0.11 -0.7
2,350,573 - srmB MCAODC_11890 0.16 -0.8
2,350,583 + srmB MCAODC_11890 0.17 -0.5
2,350,583 + srmB MCAODC_11890 0.17 +0.2
2,350,584 - srmB MCAODC_11890 0.17 -0.7
2,350,584 - srmB MCAODC_11890 0.17 +1.5
2,350,642 - srmB MCAODC_11890 0.21 +1.5
2,350,642 - srmB MCAODC_11890 0.21 -0.1
2,350,702 + srmB MCAODC_11890 0.25 -1.8
2,350,702 + srmB MCAODC_11890 0.25 -0.8
2,350,929 - srmB MCAODC_11890 0.42 -0.5
2,350,994 + srmB MCAODC_11890 0.47 -0.1
2,350,995 - srmB MCAODC_11890 0.47 -0.8
2,351,021 - srmB MCAODC_11890 0.49 +0.5
2,351,177 + srmB MCAODC_11890 0.61 -0.1
2,351,178 - srmB MCAODC_11890 0.61 -1.2
2,351,178 - srmB MCAODC_11890 0.61 -2.0
2,351,274 + srmB MCAODC_11890 0.68 +0.5
2,351,275 - srmB MCAODC_11890 0.68 +1.5
2,351,278 + srmB MCAODC_11890 0.69 -1.3
2,351,278 + srmB MCAODC_11890 0.69 +1.2
2,351,279 - srmB MCAODC_11890 0.69 +0.5
2,351,279 - srmB MCAODC_11890 0.69 +0.7
2,351,279 - srmB MCAODC_11890 0.69 +0.5

Or see this region's nucleotide sequence