Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yidC and rnpA are separated by 223 nucleotides rnpA and rpmH are separated by 49 nucleotides rpmH and ysdD are separated by 229 nucleotides ysdD and dnaA are separated by 296 nucleotides
MCAODC_05500: yidC - membrane protein insertase YidC, at 1,113,216 to 1,114,862
yidC
MCAODC_05505: rnpA - ribonuclease P protein component, at 1,115,086 to 1,115,412
rnpA
MCAODC_05510: rpmH - 50S ribosomal protein L34, at 1,115,462 to 1,115,602
rpmH
MCAODC_05515: ysdD - Protein YsdD, at 1,115,832 to 1,115,912
ysdD
MCAODC_05520: dnaA - chromosomal replication initiator protein DnaA, at 1,116,209 to 1,117,612
dnaA
Position (kb)
1115
1116 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1114.971 kb on - strand at 1115.067 kb on + strand at 1115.067 kb on + strand at 1115.068 kb on - strand at 1115.068 kb on - strand at 1115.745 kb on + strand at 1115.745 kb on + strand at 1115.749 kb on + strand at 1115.834 kb on - strand at 1115.834 kb on - strand at 1115.835 kb on + strand at 1115.835 kb on + strand at 1115.836 kb on - strand at 1115.836 kb on - strand at 1115.836 kb on - strand at 1115.850 kb on - strand, within ysdD at 1115.877 kb on - strand, within ysdD at 1115.877 kb on - strand, within ysdD at 1115.880 kb on - strand, within ysdD at 1115.904 kb on + strand, within ysdD at 1115.905 kb on - strand at 1115.934 kb on + strand at 1115.965 kb on + strand at 1115.966 kb on - strand at 1115.966 kb on - strand at 1115.972 kb on - strand at 1115.997 kb on + strand at 1115.997 kb on + strand at 1116.047 kb on + strand at 1116.047 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.091 kb on + strand at 1116.093 kb on + strand at 1116.093 kb on + strand at 1116.093 kb on + strand at 1116.144 kb on + strand at 1116.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 1,114,971 - +0.3 1,115,067 + -1.1 1,115,067 + +1.5 1,115,068 - -0.9 1,115,068 - -0.1 1,115,745 + +1.5 1,115,745 + -0.3 1,115,749 + +0.5 1,115,834 - -0.1 1,115,834 - -1.5 1,115,835 + -0.7 1,115,835 + -0.5 1,115,836 - -0.5 1,115,836 - -0.9 1,115,836 - -2.4 1,115,850 - ysdD MCAODC_05515 0.22 +2.5 1,115,877 - ysdD MCAODC_05515 0.56 -1.9 1,115,877 - ysdD MCAODC_05515 0.56 +0.7 1,115,880 - ysdD MCAODC_05515 0.59 -0.3 1,115,904 + ysdD MCAODC_05515 0.89 -0.0 1,115,905 - +0.4 1,115,934 + -0.3 1,115,965 + +1.0 1,115,966 - +0.2 1,115,966 - -1.9 1,115,972 - -0.5 1,115,997 + -0.1 1,115,997 + +0.4 1,116,047 + -1.1 1,116,047 + -1.3 1,116,091 + -0.1 1,116,091 + -0.1 1,116,091 + -0.9 1,116,091 + -1.0 1,116,091 + -0.5 1,116,091 + +1.5 1,116,091 + -1.0 1,116,091 + -0.1 1,116,091 + -0.9 1,116,093 + -0.9 1,116,093 + -0.7 1,116,093 + -1.1 1,116,144 + -0.1 1,116,173 + -0.9
Or see this region's nucleotide sequence