Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF517

Experiment: Ying_all64 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF515 and GFF516 are separated by 7 nucleotidesGFF516 and GFF517 are separated by 38 nucleotidesGFF517 and GFF518 are separated by 67 nucleotides GFF515 - Glutamine amidotransferase class-I (EC 6.3.5.2), at 182,782 to 183,522 GFF515 GFF516 - Acyl-CoA hydrolase (EC 3.1.2.20), at 183,530 to 183,970 GFF516 GFF517 - Lipid A export ATP-binding/permease protein MsbA, at 184,009 to 185,856 GFF517 GFF518 - Transcriptional regulator, at 185,924 to 186,841 GFF518 Position (kb) 184 185 186Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 183.205 kb on + strand, within GFF515at 183.205 kb on + strand, within GFF515at 183.206 kb on - strand, within GFF515at 183.380 kb on - strand, within GFF515at 183.380 kb on - strand, within GFF515at 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.487 kb on + strandat 183.607 kb on - strand, within GFF516at 183.607 kb on - strand, within GFF516at 183.607 kb on - strand, within GFF516at 183.636 kb on + strand, within GFF516at 183.636 kb on + strand, within GFF516at 183.804 kb on + strand, within GFF516at 183.804 kb on + strand, within GFF516at 184.124 kb on - strandat 184.135 kb on + strandat 184.136 kb on - strandat 184.504 kb on + strand, within GFF517at 184.505 kb on - strand, within GFF517at 184.514 kb on - strand, within GFF517at 184.586 kb on - strand, within GFF517at 184.618 kb on + strand, within GFF517at 184.618 kb on + strand, within GFF517at 184.618 kb on + strand, within GFF517at 184.619 kb on - strand, within GFF517at 184.619 kb on - strand, within GFF517at 184.619 kb on - strand, within GFF517at 184.726 kb on + strand, within GFF517at 184.726 kb on + strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.727 kb on - strand, within GFF517at 184.741 kb on + strand, within GFF517at 184.741 kb on + strand, within GFF517at 184.741 kb on + strand, within GFF517at 184.742 kb on - strand, within GFF517at 184.742 kb on - strand, within GFF517at 184.969 kb on + strand, within GFF517at 184.969 kb on + strand, within GFF517at 185.146 kb on + strand, within GFF517at 185.260 kb on + strand, within GFF517at 185.261 kb on - strand, within GFF517at 185.262 kb on - strand, within GFF517at 185.269 kb on + strand, within GFF517at 185.269 kb on + strand, within GFF517at 185.269 kb on + strand, within GFF517at 185.269 kb on + strand, within GFF517at 185.270 kb on - strand, within GFF517at 185.270 kb on - strand, within GFF517at 185.270 kb on - strand, within GFF517at 185.407 kb on + strand, within GFF517at 185.408 kb on - strand, within GFF517at 185.419 kb on + strand, within GFF517at 185.510 kb on - strand, within GFF517at 185.510 kb on - strand, within GFF517at 185.567 kb on + strand, within GFF517at 185.815 kb on + strandat 185.815 kb on + strandat 185.815 kb on + strandat 185.815 kb on + strandat 185.815 kb on + strandat 185.816 kb on - strandat 185.912 kb on + strandat 186.443 kb on + strand, within GFF518at 186.443 kb on + strand, within GFF518at 186.443 kb on + strand, within GFF518at 186.444 kb on - strand, within GFF518at 186.444 kb on - strand, within GFF518at 186.522 kb on - strand, within GFF518at 186.536 kb on + strand, within GFF518at 186.755 kb on + strandat 186.855 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 1
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183,205 + GFF515 0.57 -0.4
183,205 + GFF515 0.57 -0.1
183,206 - GFF515 0.57 -0.6
183,380 - GFF515 0.81 +0.1
183,380 - GFF515 0.81 -0.0
183,487 + -0.0
183,487 + +0.1
183,487 + +0.3
183,487 + +1.4
183,487 + -0.2
183,607 - GFF516 0.17 -0.3
183,607 - GFF516 0.17 -0.1
183,607 - GFF516 0.17 -0.1
183,636 + GFF516 0.24 -1.1
183,636 + GFF516 0.24 +1.1
183,804 + GFF516 0.62 +1.6
183,804 + GFF516 0.62 +0.4
184,124 - +0.6
184,135 + -0.8
184,136 - +0.4
184,504 + GFF517 0.27 -0.5
184,505 - GFF517 0.27 +0.0
184,514 - GFF517 0.27 -0.7
184,586 - GFF517 0.31 -0.1
184,618 + GFF517 0.33 +1.3
184,618 + GFF517 0.33 +0.2
184,618 + GFF517 0.33 -1.1
184,619 - GFF517 0.33 +0.7
184,619 - GFF517 0.33 -0.5
184,619 - GFF517 0.33 +0.1
184,726 + GFF517 0.39 -0.3
184,726 + GFF517 0.39 -0.6
184,727 - GFF517 0.39 +0.1
184,727 - GFF517 0.39 -0.6
184,727 - GFF517 0.39 +0.4
184,727 - GFF517 0.39 -0.3
184,727 - GFF517 0.39 -0.4
184,741 + GFF517 0.40 +1.2
184,741 + GFF517 0.40 -0.7
184,741 + GFF517 0.40 -0.3
184,742 - GFF517 0.40 +1.5
184,742 - GFF517 0.40 -0.3
184,969 + GFF517 0.52 -1.0
184,969 + GFF517 0.52 +0.1
185,146 + GFF517 0.62 +0.1
185,260 + GFF517 0.68 +0.5
185,261 - GFF517 0.68 -3.1
185,262 - GFF517 0.68 -0.1
185,269 + GFF517 0.68 +0.0
185,269 + GFF517 0.68 -1.7
185,269 + GFF517 0.68 -0.8
185,269 + GFF517 0.68 -0.2
185,270 - GFF517 0.68 +1.2
185,270 - GFF517 0.68 +0.5
185,270 - GFF517 0.68 -0.5
185,407 + GFF517 0.76 -0.1
185,408 - GFF517 0.76 +0.0
185,419 + GFF517 0.76 -0.1
185,510 - GFF517 0.81 -0.8
185,510 - GFF517 0.81 -0.1
185,567 + GFF517 0.84 -0.2
185,815 + +2.5
185,815 + +1.2
185,815 + -0.4
185,815 + +1.4
185,815 + -0.5
185,816 - -0.0
185,912 + +0.1
186,443 + GFF518 0.57 -1.4
186,443 + GFF518 0.57 -0.4
186,443 + GFF518 0.57 -1.1
186,444 - GFF518 0.57 -0.2
186,444 - GFF518 0.57 -1.7
186,522 - GFF518 0.65 -2.0
186,536 + GFF518 0.67 -0.3
186,755 + -1.0
186,855 + +0.0

Or see this region's nucleotide sequence