Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF162

Experiment: Ying_all64 rep B; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF161 and GFF162 are separated by 30 nucleotidesGFF162 and GFF163 are separated by 13 nucleotidesGFF163 and GFF164 are separated by 10 nucleotides GFF161 - Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4), at 174,512 to 176,452 GFF161 GFF162 - hypothetical protein, at 176,483 to 177,607 GFF162 GFF163 - hypothetical protein, at 177,621 to 177,890 GFF163 GFF164 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180), at 177,901 to 178,947 GFF164 Position (kb) 176 177 178Strain fitness (log2 ratio) -1 0 1at 176.136 kb on + strand, within GFF161at 176.217 kb on + strand, within GFF161at 176.218 kb on - strand, within GFF161at 176.218 kb on - strand, within GFF161at 176.886 kb on + strand, within GFF162at 176.886 kb on + strand, within GFF162at 177.205 kb on - strand, within GFF162at 177.429 kb on + strand, within GFF162at 177.429 kb on + strand, within GFF162at 177.429 kb on + strand, within GFF162at 177.430 kb on - strand, within GFF162at 177.998 kb on + strandat 177.998 kb on + strandat 178.292 kb on + strand, within GFF164at 178.565 kb on + strand, within GFF164at 178.566 kb on - strand, within GFF164

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 1
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176,136 + GFF161 0.84 -0.8
176,217 + GFF161 0.88 +0.0
176,218 - GFF161 0.88 +0.0
176,218 - GFF161 0.88 +1.4
176,886 + GFF162 0.36 +0.4
176,886 + GFF162 0.36 -0.8
177,205 - GFF162 0.64 +0.6
177,429 + GFF162 0.84 +0.3
177,429 + GFF162 0.84 +0.5
177,429 + GFF162 0.84 +0.4
177,430 - GFF162 0.84 -0.4
177,998 + +1.2
177,998 + -0.8
178,292 + GFF164 0.37 -0.0
178,565 + GFF164 0.63 -0.2
178,566 - GFF164 0.64 -1.0

Or see this region's nucleotide sequence