Strain Fitness in Escherichia coli ECRC101 around MCAODC_22870

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnagZ and thiK are separated by 10 nucleotidesthiK and lpoB overlap by 20 nucleotideslpoB and MCAODC_22880 are separated by 13 nucleotides MCAODC_22865: nagZ - beta-N-acetylhexosaminidase, at 1,316,252 to 1,317,277 nagZ MCAODC_22870: thiK - thiamine kinase, at 1,317,288 to 1,318,112 thiK MCAODC_22875: lpoB - penicillin-binding protein activator LpoB, at 1,318,093 to 1,318,734 lpoB MCAODC_22880: MCAODC_22880 - DUF1425 domain-containing protein, at 1,318,748 to 1,319,125 _22880 Position (kb) 1317 1318 1319Strain fitness (log2 ratio) -2 -1 0 1 2at 1316.288 kb on + strandat 1316.407 kb on + strand, within nagZat 1316.432 kb on - strand, within nagZat 1316.497 kb on - strand, within nagZat 1316.632 kb on - strand, within nagZat 1316.698 kb on + strand, within nagZat 1316.765 kb on + strand, within nagZat 1316.874 kb on + strand, within nagZat 1316.902 kb on + strand, within nagZat 1316.903 kb on - strand, within nagZat 1316.910 kb on - strand, within nagZat 1316.942 kb on - strand, within nagZat 1317.219 kb on - strandat 1317.290 kb on - strandat 1317.304 kb on - strandat 1317.304 kb on - strandat 1317.304 kb on - strandat 1317.336 kb on - strandat 1317.368 kb on - strandat 1317.430 kb on - strand, within thiKat 1317.611 kb on + strand, within thiKat 1317.612 kb on - strand, within thiKat 1317.612 kb on - strand, within thiKat 1317.625 kb on - strand, within thiKat 1317.625 kb on - strand, within thiKat 1317.778 kb on + strand, within thiKat 1317.782 kb on - strand, within thiKat 1317.868 kb on + strand, within thiKat 1317.869 kb on - strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.870 kb on + strand, within thiKat 1317.871 kb on - strand, within thiKat 1317.871 kb on - strand, within thiKat 1318.025 kb on + strand, within thiKat 1318.026 kb on - strand, within thiKat 1318.029 kb on - strand, within thiKat 1318.095 kb on - strandat 1318.095 kb on - strandat 1318.116 kb on + strandat 1318.117 kb on - strandat 1318.203 kb on - strand, within lpoBat 1318.211 kb on + strand, within lpoBat 1318.252 kb on - strand, within lpoBat 1318.285 kb on + strand, within lpoBat 1318.286 kb on - strand, within lpoBat 1318.286 kb on - strand, within lpoBat 1318.286 kb on - strand, within lpoBat 1318.467 kb on + strand, within lpoBat 1318.467 kb on + strand, within lpoBat 1318.468 kb on - strand, within lpoBat 1318.932 kb on + strand, within MCAODC_22880at 1318.932 kb on + strand, within MCAODC_22880at 1318.933 kb on - strand, within MCAODC_22880at 1319.084 kb on + strand, within MCAODC_22880at 1319.085 kb on - strand, within MCAODC_22880at 1319.085 kb on - strand, within MCAODC_22880

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,316,288 + -0.6
1,316,407 + nagZ MCAODC_22865 0.15 -0.5
1,316,432 - nagZ MCAODC_22865 0.18 -0.7
1,316,497 - nagZ MCAODC_22865 0.24 +0.2
1,316,632 - nagZ MCAODC_22865 0.37 -0.7
1,316,698 + nagZ MCAODC_22865 0.43 -1.3
1,316,765 + nagZ MCAODC_22865 0.50 -0.3
1,316,874 + nagZ MCAODC_22865 0.61 -0.4
1,316,902 + nagZ MCAODC_22865 0.63 -1.1
1,316,903 - nagZ MCAODC_22865 0.63 +0.1
1,316,910 - nagZ MCAODC_22865 0.64 +0.9
1,316,942 - nagZ MCAODC_22865 0.67 +0.9
1,317,219 - +1.4
1,317,290 - +1.5
1,317,304 - +1.7
1,317,304 - +0.1
1,317,304 - +0.0
1,317,336 - -2.7
1,317,368 - -1.5
1,317,430 - thiK MCAODC_22870 0.17 -1.2
1,317,611 + thiK MCAODC_22870 0.39 +2.1
1,317,612 - thiK MCAODC_22870 0.39 +0.1
1,317,612 - thiK MCAODC_22870 0.39 +0.3
1,317,625 - thiK MCAODC_22870 0.41 +0.2
1,317,625 - thiK MCAODC_22870 0.41 -1.1
1,317,778 + thiK MCAODC_22870 0.59 +1.1
1,317,782 - thiK MCAODC_22870 0.60 +1.7
1,317,868 + thiK MCAODC_22870 0.70 +0.3
1,317,869 - thiK MCAODC_22870 0.70 +2.7
1,317,870 + thiK MCAODC_22870 0.71 +0.7
1,317,870 + thiK MCAODC_22870 0.71 +0.1
1,317,870 + thiK MCAODC_22870 0.71 -0.8
1,317,870 + thiK MCAODC_22870 0.71 -0.3
1,317,871 - thiK MCAODC_22870 0.71 -1.1
1,317,871 - thiK MCAODC_22870 0.71 -0.1
1,318,025 + thiK MCAODC_22870 0.89 -0.5
1,318,026 - thiK MCAODC_22870 0.89 +1.7
1,318,029 - thiK MCAODC_22870 0.90 +1.1
1,318,095 - -0.8
1,318,095 - -0.6
1,318,116 + +0.7
1,318,117 - -0.3
1,318,203 - lpoB MCAODC_22875 0.17 +0.1
1,318,211 + lpoB MCAODC_22875 0.18 -0.4
1,318,252 - lpoB MCAODC_22875 0.25 +1.7
1,318,285 + lpoB MCAODC_22875 0.30 -0.9
1,318,286 - lpoB MCAODC_22875 0.30 -1.3
1,318,286 - lpoB MCAODC_22875 0.30 +0.1
1,318,286 - lpoB MCAODC_22875 0.30 -2.2
1,318,467 + lpoB MCAODC_22875 0.58 -0.6
1,318,467 + lpoB MCAODC_22875 0.58 +0.4
1,318,468 - lpoB MCAODC_22875 0.58 +0.7
1,318,932 + MCAODC_22880 0.49 -1.1
1,318,932 + MCAODC_22880 0.49 +0.1
1,318,933 - MCAODC_22880 0.49 -0.4
1,319,084 + MCAODC_22880 0.89 -0.3
1,319,085 - MCAODC_22880 0.89 -0.9
1,319,085 - MCAODC_22880 0.89 +1.7

Or see this region's nucleotide sequence