Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potA and potB overlap by 17 nucleotides potB and espK are separated by 363 nucleotides
MCAODC_22460: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 1,251,290 to 1,252,426
potA
MCAODC_22465: potB - spermidine/putrescine ABC transporter permease PotB, at 1,252,410 to 1,253,273
potB
MCAODC_22470: espK - type III secretion system effector EspK, at 1,253,637 to 1,254,998
espK
Position (kb)
1252
1253
1254 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1251.581 kb on + strand, within potA at 1251.581 kb on + strand, within potA at 1251.886 kb on + strand, within potA at 1251.887 kb on - strand, within potA at 1252.045 kb on + strand, within potA at 1252.117 kb on + strand, within potA at 1252.117 kb on + strand, within potA at 1252.120 kb on + strand, within potA at 1252.120 kb on + strand, within potA at 1252.124 kb on + strand, within potA at 1252.125 kb on - strand, within potA at 1252.125 kb on - strand, within potA at 1252.185 kb on + strand, within potA at 1252.186 kb on - strand, within potA at 1252.235 kb on + strand, within potA at 1252.250 kb on - strand, within potA at 1252.254 kb on - strand, within potA at 1252.375 kb on + strand at 1252.424 kb on + strand at 1252.438 kb on + strand at 1252.566 kb on + strand, within potB at 1252.603 kb on + strand, within potB at 1252.941 kb on - strand, within potB at 1252.994 kb on + strand, within potB at 1253.076 kb on + strand, within potB at 1253.076 kb on + strand, within potB at 1253.077 kb on - strand, within potB at 1253.077 kb on - strand, within potB at 1253.077 kb on - strand, within potB at 1253.134 kb on + strand, within potB at 1253.134 kb on + strand, within potB at 1253.147 kb on + strand, within potB at 1253.184 kb on - strand, within potB at 1253.349 kb on - strand at 1253.450 kb on + strand at 1253.762 kb on - strand at 1253.822 kb on + strand, within espK at 1254.021 kb on + strand, within espK at 1254.022 kb on - strand, within espK at 1254.023 kb on + strand, within espK at 1254.024 kb on - strand, within espK at 1254.025 kb on + strand, within espK at 1254.129 kb on + strand, within espK at 1254.130 kb on - strand, within espK at 1254.130 kb on - strand, within espK at 1254.210 kb on - strand, within espK at 1254.212 kb on + strand, within espK at 1254.213 kb on - strand, within espK at 1254.215 kb on - strand, within espK
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 1,251,581 + potA MCAODC_22460 0.26 +1.2 1,251,581 + potA MCAODC_22460 0.26 +0.2 1,251,886 + potA MCAODC_22460 0.52 +0.2 1,251,887 - potA MCAODC_22460 0.53 -0.9 1,252,045 + potA MCAODC_22460 0.66 -1.5 1,252,117 + potA MCAODC_22460 0.73 -1.0 1,252,117 + potA MCAODC_22460 0.73 +0.7 1,252,120 + potA MCAODC_22460 0.73 -0.9 1,252,120 + potA MCAODC_22460 0.73 +1.2 1,252,124 + potA MCAODC_22460 0.73 -0.9 1,252,125 - potA MCAODC_22460 0.73 +0.3 1,252,125 - potA MCAODC_22460 0.73 -0.9 1,252,185 + potA MCAODC_22460 0.79 -0.3 1,252,186 - potA MCAODC_22460 0.79 +1.7 1,252,235 + potA MCAODC_22460 0.83 +2.5 1,252,250 - potA MCAODC_22460 0.84 +0.1 1,252,254 - potA MCAODC_22460 0.85 -1.3 1,252,375 + -0.3 1,252,424 + +0.7 1,252,438 + -0.3 1,252,566 + potB MCAODC_22465 0.18 +0.5 1,252,603 + potB MCAODC_22465 0.22 +2.7 1,252,941 - potB MCAODC_22465 0.61 +0.1 1,252,994 + potB MCAODC_22465 0.68 -0.3 1,253,076 + potB MCAODC_22465 0.77 -0.7 1,253,076 + potB MCAODC_22465 0.77 +2.7 1,253,077 - potB MCAODC_22465 0.77 +0.4 1,253,077 - potB MCAODC_22465 0.77 +1.7 1,253,077 - potB MCAODC_22465 0.77 +3.2 1,253,134 + potB MCAODC_22465 0.84 -0.9 1,253,134 + potB MCAODC_22465 0.84 +0.7 1,253,147 + potB MCAODC_22465 0.85 +1.1 1,253,184 - potB MCAODC_22465 0.90 +0.1 1,253,349 - -0.0 1,253,450 + +0.1 1,253,762 - +1.1 1,253,822 + espK MCAODC_22470 0.14 +2.2 1,254,021 + espK MCAODC_22470 0.28 +1.7 1,254,022 - espK MCAODC_22470 0.28 +0.7 1,254,023 + espK MCAODC_22470 0.28 +0.3 1,254,024 - espK MCAODC_22470 0.28 +0.2 1,254,025 + espK MCAODC_22470 0.28 -0.9 1,254,129 + espK MCAODC_22470 0.36 -0.3 1,254,130 - espK MCAODC_22470 0.36 +0.7 1,254,130 - espK MCAODC_22470 0.36 +0.1 1,254,210 - espK MCAODC_22470 0.42 +1.7 1,254,212 + espK MCAODC_22470 0.42 -0.5 1,254,213 - espK MCAODC_22470 0.42 -2.4 1,254,215 - espK MCAODC_22470 0.42 +0.1
Or see this region's nucleotide sequence