Strain Fitness in Escherichia coli ECRC101 around MCAODC_21830

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlolB and ispE overlap by 1 nucleotidesispE and prs are separated by 84 nucleotidesprs and dauA are separated by 151 nucleotides MCAODC_21820: lolB - lipoprotein insertase outer membrane protein LolB, at 1,146,472 to 1,147,095 lolB MCAODC_21825: ispE - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 1,147,095 to 1,147,946 ispE MCAODC_21830: prs - ribose-phosphate diphosphokinase, at 1,148,031 to 1,149,044 prs MCAODC_21835: dauA - C4-dicarboxylic acid transporter DauA, at 1,149,196 to 1,150,848 dauA Position (kb) 1148 1149 1150Strain fitness (log2 ratio) -1 0 1 2at 1149.166 kb on - strandat 1149.238 kb on + strandat 1149.239 kb on - strandat 1149.240 kb on + strandat 1149.278 kb on + strandat 1149.353 kb on + strandat 1149.353 kb on + strandat 1149.355 kb on + strandat 1149.356 kb on - strandat 1149.358 kb on - strandat 1149.409 kb on - strand, within dauAat 1149.668 kb on - strand, within dauAat 1149.668 kb on - strand, within dauAat 1149.671 kb on + strand, within dauAat 1149.694 kb on - strand, within dauAat 1149.746 kb on + strand, within dauAat 1149.746 kb on + strand, within dauAat 1149.750 kb on + strand, within dauAat 1149.837 kb on + strand, within dauAat 1149.861 kb on + strand, within dauAat 1149.883 kb on + strand, within dauAat 1149.884 kb on - strand, within dauAat 1149.884 kb on - strand, within dauA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,149,166 - +0.3
1,149,238 + +0.3
1,149,239 - +1.2
1,149,240 + +0.9
1,149,278 + -0.3
1,149,353 + +0.2
1,149,353 + +2.7
1,149,355 + +0.7
1,149,356 - -0.7
1,149,358 - +0.1
1,149,409 - dauA MCAODC_21835 0.13 -0.6
1,149,668 - dauA MCAODC_21835 0.29 -1.1
1,149,668 - dauA MCAODC_21835 0.29 +0.1
1,149,671 + dauA MCAODC_21835 0.29 +2.2
1,149,694 - dauA MCAODC_21835 0.30 +0.4
1,149,746 + dauA MCAODC_21835 0.33 +1.5
1,149,746 + dauA MCAODC_21835 0.33 -0.7
1,149,750 + dauA MCAODC_21835 0.34 +1.7
1,149,837 + dauA MCAODC_21835 0.39 -0.9
1,149,861 + dauA MCAODC_21835 0.40 +0.7
1,149,883 + dauA MCAODC_21835 0.42 -1.0
1,149,884 - dauA MCAODC_21835 0.42 +0.3
1,149,884 - dauA MCAODC_21835 0.42 -1.7

Or see this region's nucleotide sequence