Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mASE1 and alkA are separated by 100 nucleotides alkA and yegD are separated by 181 nucleotides
MCAODC_20680: mASE1 - diguanylate cyclase, at 947,514 to 950,321
mASE1
MCAODC_20685: alkA - DNA-3-methyladenine glycosylase 2, at 950,422 to 951,270
alkA
MCAODC_20690: yegD - molecular chaperone, at 951,452 to 952,756
yegD
Position (kb) 950
951
952 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 949.491 kb on + strand, within mASE1 at 949.492 kb on - strand, within mASE1 at 949.531 kb on - strand, within mASE1 at 949.686 kb on - strand, within mASE1 at 949.712 kb on + strand, within mASE1 at 949.737 kb on - strand, within mASE1 at 949.758 kb on - strand, within mASE1 at 949.831 kb on + strand, within mASE1 at 949.933 kb on + strand, within mASE1 at 949.933 kb on + strand, within mASE1 at 949.934 kb on - strand, within mASE1 at 949.934 kb on - strand, within mASE1 at 950.066 kb on + strand at 950.076 kb on + strand at 950.076 kb on + strand at 950.077 kb on - strand at 950.098 kb on + strand at 950.098 kb on + strand at 950.117 kb on + strand at 950.141 kb on + strand at 950.203 kb on + strand at 950.220 kb on - strand at 950.297 kb on + strand at 950.297 kb on + strand at 950.298 kb on - strand at 950.301 kb on - strand at 950.301 kb on - strand at 950.305 kb on - strand at 950.460 kb on + strand at 950.460 kb on + strand at 950.461 kb on - strand at 950.461 kb on - strand at 950.475 kb on - strand at 950.556 kb on - strand, within alkA at 950.690 kb on - strand, within alkA at 950.752 kb on - strand, within alkA at 950.752 kb on - strand, within alkA at 950.752 kb on - strand, within alkA at 950.755 kb on + strand, within alkA at 950.764 kb on + strand, within alkA at 950.765 kb on - strand, within alkA at 950.765 kb on - strand, within alkA at 950.765 kb on - strand, within alkA at 950.815 kb on - strand, within alkA at 950.815 kb on - strand, within alkA at 950.815 kb on - strand, within alkA at 950.815 kb on - strand, within alkA at 950.817 kb on - strand, within alkA at 950.847 kb on - strand, within alkA at 951.070 kb on + strand, within alkA at 951.089 kb on - strand, within alkA at 951.089 kb on - strand, within alkA at 951.168 kb on - strand, within alkA at 951.170 kb on + strand, within alkA at 951.170 kb on + strand, within alkA at 951.265 kb on - strand at 951.266 kb on + strand at 951.266 kb on + strand at 951.266 kb on + strand at 951.266 kb on + strand at 951.266 kb on + strand at 951.267 kb on - strand at 951.267 kb on - strand at 951.267 kb on - strand at 951.282 kb on - strand at 951.301 kb on - strand at 951.333 kb on + strand at 951.398 kb on + strand at 951.398 kb on + strand at 951.398 kb on + strand at 951.399 kb on - strand at 951.399 kb on - strand at 951.409 kb on + strand at 951.423 kb on - strand at 951.464 kb on - strand at 951.620 kb on + strand, within yegD at 951.622 kb on + strand, within yegD at 951.622 kb on + strand, within yegD at 951.689 kb on + strand, within yegD at 951.689 kb on + strand, within yegD at 951.689 kb on + strand, within yegD at 951.689 kb on + strand, within yegD at 951.689 kb on + strand, within yegD at 951.689 kb on + strand, within yegD at 951.771 kb on + strand, within yegD at 951.772 kb on - strand, within yegD at 951.851 kb on - strand, within yegD at 951.851 kb on - strand, within yegD at 951.969 kb on + strand, within yegD at 951.970 kb on - strand, within yegD at 951.970 kb on - strand, within yegD at 952.018 kb on - strand, within yegD at 952.060 kb on + strand, within yegD at 952.060 kb on + strand, within yegD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 949,491 + mASE1 MCAODC_20680 0.70 +1.1 949,492 - mASE1 MCAODC_20680 0.70 +1.4 949,531 - mASE1 MCAODC_20680 0.72 -1.2 949,686 - mASE1 MCAODC_20680 0.77 -0.3 949,712 + mASE1 MCAODC_20680 0.78 -0.3 949,737 - mASE1 MCAODC_20680 0.79 +2.6 949,758 - mASE1 MCAODC_20680 0.80 +0.1 949,831 + mASE1 MCAODC_20680 0.83 -3.0 949,933 + mASE1 MCAODC_20680 0.86 +0.9 949,933 + mASE1 MCAODC_20680 0.86 +0.4 949,934 - mASE1 MCAODC_20680 0.86 -0.7 949,934 - mASE1 MCAODC_20680 0.86 -1.6 950,066 + -0.1 950,076 + -0.7 950,076 + +0.6 950,077 - -1.0 950,098 + +2.9 950,098 + -1.8 950,117 + -1.2 950,141 + -1.1 950,203 + +0.3 950,220 - +1.6 950,297 + -0.2 950,297 + -0.4 950,298 - +0.3 950,301 - -1.6 950,301 - -0.1 950,305 - -1.7 950,460 + +0.9 950,460 + +0.2 950,461 - +2.2 950,461 - -0.1 950,475 - +0.4 950,556 - alkA MCAODC_20685 0.16 -0.2 950,690 - alkA MCAODC_20685 0.32 +0.0 950,752 - alkA MCAODC_20685 0.39 +0.0 950,752 - alkA MCAODC_20685 0.39 -1.6 950,752 - alkA MCAODC_20685 0.39 +0.8 950,755 + alkA MCAODC_20685 0.39 -0.7 950,764 + alkA MCAODC_20685 0.40 -0.7 950,765 - alkA MCAODC_20685 0.40 +0.1 950,765 - alkA MCAODC_20685 0.40 +1.2 950,765 - alkA MCAODC_20685 0.40 +0.5 950,815 - alkA MCAODC_20685 0.46 +0.4 950,815 - alkA MCAODC_20685 0.46 +0.3 950,815 - alkA MCAODC_20685 0.46 -0.8 950,815 - alkA MCAODC_20685 0.46 +0.5 950,817 - alkA MCAODC_20685 0.47 -0.4 950,847 - alkA MCAODC_20685 0.50 +1.0 951,070 + alkA MCAODC_20685 0.76 +1.3 951,089 - alkA MCAODC_20685 0.79 +0.5 951,089 - alkA MCAODC_20685 0.79 +0.4 951,168 - alkA MCAODC_20685 0.88 -1.0 951,170 + alkA MCAODC_20685 0.88 +1.2 951,170 + alkA MCAODC_20685 0.88 +0.6 951,265 - +0.7 951,266 + +1.0 951,266 + +0.3 951,266 + -0.4 951,266 + -0.9 951,266 + -1.2 951,267 - -3.8 951,267 - -0.3 951,267 - +1.1 951,282 - -0.3 951,301 - +0.0 951,333 + +2.1 951,398 + -1.6 951,398 + +1.3 951,398 + +1.6 951,399 - +0.3 951,399 - -1.3 951,409 + -0.3 951,423 - +1.2 951,464 - +0.2 951,620 + yegD MCAODC_20690 0.13 -1.6 951,622 + yegD MCAODC_20690 0.13 +0.3 951,622 + yegD MCAODC_20690 0.13 +0.9 951,689 + yegD MCAODC_20690 0.18 -0.6 951,689 + yegD MCAODC_20690 0.18 +0.0 951,689 + yegD MCAODC_20690 0.18 +0.2 951,689 + yegD MCAODC_20690 0.18 -0.8 951,689 + yegD MCAODC_20690 0.18 +0.0 951,689 + yegD MCAODC_20690 0.18 +2.2 951,771 + yegD MCAODC_20690 0.24 +0.1 951,772 - yegD MCAODC_20690 0.25 +1.0 951,851 - yegD MCAODC_20690 0.31 +0.6 951,851 - yegD MCAODC_20690 0.31 +0.8 951,969 + yegD MCAODC_20690 0.40 +0.5 951,970 - yegD MCAODC_20690 0.40 -2.1 951,970 - yegD MCAODC_20690 0.40 +3.6 952,018 - yegD MCAODC_20690 0.43 -0.4 952,060 + yegD MCAODC_20690 0.47 +1.0 952,060 + yegD MCAODC_20690 0.47 +0.4
Or see this region's nucleotide sequence