Strain Fitness in Escherichia coli ECRC101 around MCAODC_20555

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaL and wcaK overlap by 4 nucleotideswcaK and wzxC are separated by 179 nucleotides MCAODC_20550: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 919,177 to 920,397 wcaL MCAODC_20555: wcaK - colanic acid biosynthesis pyruvyl transferase WcaK, at 920,394 to 921,674 wcaK MCAODC_20560: wzxC - colanic acid undecaprenyl disphosphate flippase WzxC, at 921,854 to 923,332 wzxC Position (kb) 920 921 922Strain fitness (log2 ratio) -2 -1 0 1 2at 919.398 kb on + strand, within wcaLat 919.459 kb on - strand, within wcaLat 919.459 kb on - strand, within wcaLat 919.574 kb on + strand, within wcaLat 919.777 kb on - strand, within wcaLat 919.777 kb on - strand, within wcaLat 919.777 kb on - strand, within wcaLat 919.781 kb on - strand, within wcaLat 919.781 kb on - strand, within wcaLat 919.781 kb on - strand, within wcaLat 919.907 kb on + strand, within wcaLat 919.907 kb on + strand, within wcaLat 919.908 kb on - strand, within wcaLat 919.935 kb on + strand, within wcaLat 919.936 kb on - strand, within wcaLat 919.936 kb on - strand, within wcaLat 920.136 kb on + strand, within wcaLat 920.244 kb on - strand, within wcaLat 920.247 kb on + strand, within wcaLat 920.421 kb on + strandat 920.505 kb on + strandat 920.511 kb on - strandat 920.541 kb on + strand, within wcaKat 920.606 kb on + strand, within wcaKat 920.606 kb on + strand, within wcaKat 920.607 kb on - strand, within wcaKat 920.615 kb on - strand, within wcaKat 920.615 kb on - strand, within wcaKat 920.654 kb on + strand, within wcaKat 920.742 kb on + strand, within wcaKat 920.810 kb on + strand, within wcaKat 920.810 kb on + strand, within wcaKat 920.810 kb on + strand, within wcaKat 920.812 kb on + strand, within wcaKat 920.813 kb on - strand, within wcaKat 920.902 kb on + strand, within wcaKat 920.957 kb on + strand, within wcaKat 921.011 kb on - strand, within wcaKat 921.089 kb on + strand, within wcaKat 921.176 kb on + strand, within wcaKat 921.218 kb on + strand, within wcaKat 921.239 kb on + strand, within wcaKat 921.239 kb on + strand, within wcaKat 921.277 kb on + strand, within wcaKat 921.277 kb on + strand, within wcaKat 921.312 kb on - strand, within wcaKat 921.411 kb on + strand, within wcaKat 921.411 kb on + strand, within wcaKat 921.424 kb on + strand, within wcaKat 921.575 kb on + strandat 921.607 kb on - strandat 921.607 kb on - strandat 921.607 kb on - strandat 921.666 kb on + strandat 921.761 kb on - strandat 921.769 kb on + strandat 921.793 kb on - strandat 921.975 kb on - strandat 922.035 kb on + strand, within wzxCat 922.035 kb on + strand, within wzxCat 922.065 kb on + strand, within wzxCat 922.066 kb on - strand, within wzxCat 922.217 kb on - strand, within wzxCat 922.316 kb on + strand, within wzxCat 922.317 kb on - strand, within wzxCat 922.585 kb on - strand, within wzxCat 922.597 kb on - strand, within wzxCat 922.663 kb on - strand, within wzxCat 922.663 kb on - strand, within wzxC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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919,398 + wcaL MCAODC_20550 0.18 +1.6
919,459 - wcaL MCAODC_20550 0.23 -1.6
919,459 - wcaL MCAODC_20550 0.23 +0.1
919,574 + wcaL MCAODC_20550 0.33 -0.2
919,777 - wcaL MCAODC_20550 0.49 +0.6
919,777 - wcaL MCAODC_20550 0.49 -1.6
919,777 - wcaL MCAODC_20550 0.49 -0.1
919,781 - wcaL MCAODC_20550 0.49 -0.1
919,781 - wcaL MCAODC_20550 0.49 -1.3
919,781 - wcaL MCAODC_20550 0.49 +0.5
919,907 + wcaL MCAODC_20550 0.60 +0.4
919,907 + wcaL MCAODC_20550 0.60 -0.3
919,908 - wcaL MCAODC_20550 0.60 -0.3
919,935 + wcaL MCAODC_20550 0.62 +0.4
919,936 - wcaL MCAODC_20550 0.62 -0.8
919,936 - wcaL MCAODC_20550 0.62 -1.9
920,136 + wcaL MCAODC_20550 0.79 +2.3
920,244 - wcaL MCAODC_20550 0.87 +0.5
920,247 + wcaL MCAODC_20550 0.88 -0.9
920,421 + +0.6
920,505 + -0.5
920,511 - +0.1
920,541 + wcaK MCAODC_20555 0.11 +0.2
920,606 + wcaK MCAODC_20555 0.17 -2.3
920,606 + wcaK MCAODC_20555 0.17 +1.6
920,607 - wcaK MCAODC_20555 0.17 +0.2
920,615 - wcaK MCAODC_20555 0.17 -1.0
920,615 - wcaK MCAODC_20555 0.17 -0.4
920,654 + wcaK MCAODC_20555 0.20 -1.4
920,742 + wcaK MCAODC_20555 0.27 +0.0
920,810 + wcaK MCAODC_20555 0.32 +0.6
920,810 + wcaK MCAODC_20555 0.32 +0.6
920,810 + wcaK MCAODC_20555 0.32 -1.0
920,812 + wcaK MCAODC_20555 0.33 -0.5
920,813 - wcaK MCAODC_20555 0.33 -1.2
920,902 + wcaK MCAODC_20555 0.40 -1.2
920,957 + wcaK MCAODC_20555 0.44 +0.5
921,011 - wcaK MCAODC_20555 0.48 -0.2
921,089 + wcaK MCAODC_20555 0.54 -0.6
921,176 + wcaK MCAODC_20555 0.61 +0.3
921,218 + wcaK MCAODC_20555 0.64 -0.3
921,239 + wcaK MCAODC_20555 0.66 +0.6
921,239 + wcaK MCAODC_20555 0.66 +0.4
921,277 + wcaK MCAODC_20555 0.69 +0.0
921,277 + wcaK MCAODC_20555 0.69 -0.3
921,312 - wcaK MCAODC_20555 0.72 +0.3
921,411 + wcaK MCAODC_20555 0.79 -0.5
921,411 + wcaK MCAODC_20555 0.79 +0.9
921,424 + wcaK MCAODC_20555 0.80 -1.1
921,575 + +0.6
921,607 - -0.2
921,607 - -0.1
921,607 - +0.0
921,666 + +0.9
921,761 - +0.3
921,769 + +0.6
921,793 - -0.1
921,975 - -0.4
922,035 + wzxC MCAODC_20560 0.12 -0.7
922,035 + wzxC MCAODC_20560 0.12 -0.1
922,065 + wzxC MCAODC_20560 0.14 -1.6
922,066 - wzxC MCAODC_20560 0.14 +0.9
922,217 - wzxC MCAODC_20560 0.25 +1.0
922,316 + wzxC MCAODC_20560 0.31 -0.3
922,317 - wzxC MCAODC_20560 0.31 +0.6
922,585 - wzxC MCAODC_20560 0.49 +1.4
922,597 - wzxC MCAODC_20560 0.50 +1.0
922,663 - wzxC MCAODC_20560 0.55 -0.9
922,663 - wzxC MCAODC_20560 0.55 -1.0

Or see this region's nucleotide sequence