Strain Fitness in Escherichia coli ECRC101 around MCAODC_20510

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrfaB and rfbE are separated by 24 nucleotidesrfbE and wzx are separated by 2 nucleotides MCAODC_20505: rfaB - glycosyl transferase, at 908,772 to 909,986 rfaB MCAODC_20510: rfbE - GDP-perosamine synthase RfbE/PerA, at 910,011 to 911,105 rfbE MCAODC_20515: wzx - O157 family O-antigen flippase, at 911,108 to 912,499 wzx Position (kb) 910 911 912Strain fitness (log2 ratio) -2 -1 0 1at 909.117 kb on - strand, within rfaBat 909.674 kb on - strand, within rfaBat 910.056 kb on - strandat 910.248 kb on - strand, within rfbEat 910.855 kb on - strand, within rfbEat 911.124 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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909,117 - rfaB MCAODC_20505 0.28 -0.7
909,674 - rfaB MCAODC_20505 0.74 +0.6
910,056 - +1.6
910,248 - rfbE MCAODC_20510 0.22 +0.9
910,855 - rfbE MCAODC_20510 0.77 +0.6
911,124 - -2.0

Or see this region's nucleotide sequence