Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manY and manZ are separated by 12 nucleotides manZ and MCAODC_18890 are separated by 54 nucleotides MCAODC_18890 and MCAODC_18895 are separated by 310 nucleotides MCAODC_18895 and mntP overlap by 4 nucleotides mntP and rlmA overlap by 4 nucleotides
MCAODC_18880: manY - PTS mannose transporter subunit IIC, at 634,179 to 634,979
manY
MCAODC_18885: manZ - PTS mannose transporter subunit IID, at 634,992 to 635,843
manZ
MCAODC_18890: MCAODC_18890 - UPF0266 membrane protein YobD, at 635,898 to 636,356
_18890
MCAODC_18895: MCAODC_18895 - Membrane protein, at 636,667 to 636,789
_18895
MCAODC_18900: mntP - manganese efflux pump MntP, at 636,786 to 637,352
mntP
MCAODC_18905: rlmA - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 637,349 to 638,158
rlmA
Position (kb) 635
636
637 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 634.901 kb on + strand at 634.911 kb on + strand at 635.000 kb on + strand at 635.012 kb on + strand at 635.042 kb on + strand at 635.099 kb on + strand, within manZ at 635.099 kb on + strand, within manZ at 635.099 kb on + strand, within manZ at 635.126 kb on + strand, within manZ at 635.183 kb on + strand, within manZ at 635.273 kb on + strand, within manZ at 635.273 kb on + strand, within manZ at 635.344 kb on + strand, within manZ at 635.371 kb on + strand, within manZ at 635.371 kb on + strand, within manZ at 635.372 kb on - strand, within manZ at 635.372 kb on - strand, within manZ at 635.499 kb on + strand, within manZ at 635.516 kb on + strand, within manZ at 635.528 kb on + strand, within manZ at 635.528 kb on + strand, within manZ at 635.528 kb on + strand, within manZ at 635.744 kb on + strand, within manZ at 635.745 kb on - strand, within manZ at 635.745 kb on - strand, within manZ at 635.852 kb on + strand at 635.852 kb on + strand at 636.072 kb on + strand, within MCAODC_18890 at 636.075 kb on - strand, within MCAODC_18890 at 636.145 kb on - strand, within MCAODC_18890 at 636.179 kb on + strand, within MCAODC_18890 at 636.572 kb on + strand at 636.610 kb on + strand at 636.633 kb on + strand at 636.666 kb on + strand at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.727 kb on + strand, within MCAODC_18895 at 636.759 kb on + strand, within MCAODC_18895 at 636.759 kb on + strand, within MCAODC_18895 at 636.759 kb on + strand, within MCAODC_18895 at 636.801 kb on - strand at 637.040 kb on + strand, within mntP at 637.184 kb on + strand, within mntP at 637.223 kb on + strand, within mntP at 637.223 kb on + strand, within mntP at 637.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 634,901 + +0.8 634,911 + -0.6 635,000 + -0.2 635,012 + -1.0 635,042 + +0.6 635,099 + manZ MCAODC_18885 0.13 +0.3 635,099 + manZ MCAODC_18885 0.13 -0.2 635,099 + manZ MCAODC_18885 0.13 +1.4 635,126 + manZ MCAODC_18885 0.16 -1.7 635,183 + manZ MCAODC_18885 0.22 +3.6 635,273 + manZ MCAODC_18885 0.33 -1.2 635,273 + manZ MCAODC_18885 0.33 +0.9 635,344 + manZ MCAODC_18885 0.41 +0.7 635,371 + manZ MCAODC_18885 0.44 -0.1 635,371 + manZ MCAODC_18885 0.44 -0.5 635,372 - manZ MCAODC_18885 0.45 -0.4 635,372 - manZ MCAODC_18885 0.45 +1.6 635,499 + manZ MCAODC_18885 0.60 +0.1 635,516 + manZ MCAODC_18885 0.62 +1.0 635,528 + manZ MCAODC_18885 0.63 +0.6 635,528 + manZ MCAODC_18885 0.63 -1.3 635,528 + manZ MCAODC_18885 0.63 -1.0 635,744 + manZ MCAODC_18885 0.88 +0.3 635,745 - manZ MCAODC_18885 0.88 -2.0 635,745 - manZ MCAODC_18885 0.88 +1.6 635,852 + -0.4 635,852 + +0.3 636,072 + MCAODC_18890 0.38 +2.2 636,075 - MCAODC_18890 0.39 -0.5 636,145 - MCAODC_18890 0.54 +1.6 636,179 + MCAODC_18890 0.61 +0.0 636,572 + -0.4 636,610 + +0.6 636,633 + -0.1 636,666 + -0.1 636,727 + MCAODC_18895 0.49 +0.5 636,727 + MCAODC_18895 0.49 +0.5 636,727 + MCAODC_18895 0.49 -1.1 636,727 + MCAODC_18895 0.49 +1.0 636,727 + MCAODC_18895 0.49 -1.4 636,727 + MCAODC_18895 0.49 -1.6 636,727 + MCAODC_18895 0.49 -0.7 636,727 + MCAODC_18895 0.49 -0.2 636,759 + MCAODC_18895 0.75 -0.3 636,759 + MCAODC_18895 0.75 +1.0 636,759 + MCAODC_18895 0.75 +0.3 636,801 - +0.6 637,040 + mntP MCAODC_18900 0.45 +1.8 637,184 + mntP MCAODC_18900 0.70 +1.4 637,223 + mntP MCAODC_18900 0.77 -0.4 637,223 + mntP MCAODC_18900 0.77 -0.4 637,354 - +1.0
Or see this region's nucleotide sequence