Strain Fitness in Escherichia coli ECRC101 around MCAODC_18630

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-IS629 and MCAODC_18625 overlap by 1 nucleotidesMCAODC_18625 and MCAODC_18630 overlap by 26 nucleotidesMCAODC_18630 and ydjF are separated by 117 nucleotides MCAODC_18620: tnp-IS629 - IS3 family IS629 transposase, at 583,505 to 583,831 tnp-IS629 MCAODC_18625: MCAODC_18625 - IS3 family transposase, at 583,831 to 584,718 _18625 MCAODC_18630: MCAODC_18630 - Insertion sequence 2 OrfA protein, at 584,693 to 584,863 _18630 MCAODC_18635: ydjF - Uncharacterized HTH-type transcriptional regulator YdjF, at 584,981 to 585,739 ydjF Position (kb) 584 585Strain fitness (log2 ratio) -1 0 1 2at 584.777 kb on - strand, within MCAODC_18630at 585.058 kb on - strand, within ydjFat 585.208 kb on + strand, within ydjFat 585.221 kb on - strand, within ydjFat 585.221 kb on - strand, within ydjFat 585.231 kb on - strand, within ydjFat 585.395 kb on - strand, within ydjFat 585.494 kb on - strand, within ydjFat 585.510 kb on + strand, within ydjFat 585.532 kb on - strand, within ydjFat 585.583 kb on - strand, within ydjFat 585.583 kb on - strand, within ydjFat 585.710 kb on - strandat 585.744 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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584,777 - MCAODC_18630 0.49 +1.6
585,058 - ydjF MCAODC_18635 0.10 +2.6
585,208 + ydjF MCAODC_18635 0.30 -0.4
585,221 - ydjF MCAODC_18635 0.32 +0.3
585,221 - ydjF MCAODC_18635 0.32 +1.6
585,231 - ydjF MCAODC_18635 0.33 -1.0
585,395 - ydjF MCAODC_18635 0.55 +0.1
585,494 - ydjF MCAODC_18635 0.68 -1.0
585,510 + ydjF MCAODC_18635 0.70 -1.3
585,532 - ydjF MCAODC_18635 0.73 -1.6
585,583 - ydjF MCAODC_18635 0.79 -0.4
585,583 - ydjF MCAODC_18635 0.79 -1.4
585,710 - -0.4
585,744 - +1.0

Or see this region's nucleotide sequence