Strain Fitness in Escherichia coli ECRC101 around MCAODC_18305

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnlpC and btuD are separated by 77 nucleotidesbtuD and btuE overlap by 1 nucleotidesbtuE and btuC are separated by 62 nucleotides MCAODC_18295: nlpC - putative endopeptidase NlpC, at 521,249 to 521,713 nlpC MCAODC_18300: btuD - vitamin B12 ABC transporter ATP-binding protein BtuD, at 521,791 to 522,540 btuD MCAODC_18305: btuE - bifunctional thioredoxin/glutathione peroxidase, at 522,540 to 523,091 btuE MCAODC_18310: btuC - vitamin B12 ABC transporter permease BtuC, at 523,154 to 524,134 btuC Position (kb) 522 523 524Strain fitness (log2 ratio) -1 0 1at 521.589 kb on - strand, within nlpCat 521.924 kb on - strand, within btuDat 522.224 kb on - strand, within btuDat 522.520 kb on + strandat 522.521 kb on - strandat 522.561 kb on - strandat 522.597 kb on - strand, within btuEat 523.124 kb on - strandat 523.242 kb on - strandat 523.349 kb on - strand, within btuCat 523.494 kb on - strand, within btuCat 523.494 kb on - strand, within btuCat 523.499 kb on - strand, within btuCat 523.499 kb on - strand, within btuCat 523.499 kb on - strand, within btuCat 523.578 kb on - strand, within btuCat 523.606 kb on - strand, within btuCat 523.620 kb on - strand, within btuCat 523.645 kb on + strand, within btuCat 523.743 kb on - strand, within btuCat 523.756 kb on + strand, within btuCat 523.866 kb on - strand, within btuCat 524.048 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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521,589 - nlpC MCAODC_18295 0.73 -1.0
521,924 - btuD MCAODC_18300 0.18 +0.2
522,224 - btuD MCAODC_18300 0.58 -0.4
522,520 + +0.3
522,521 - -0.5
522,561 - +0.6
522,597 - btuE MCAODC_18305 0.10 +1.6
523,124 - +0.0
523,242 - -0.7
523,349 - btuC MCAODC_18310 0.20 +1.2
523,494 - btuC MCAODC_18310 0.35 -0.3
523,494 - btuC MCAODC_18310 0.35 +0.2
523,499 - btuC MCAODC_18310 0.35 +1.3
523,499 - btuC MCAODC_18310 0.35 -0.7
523,499 - btuC MCAODC_18310 0.35 +1.3
523,578 - btuC MCAODC_18310 0.43 +0.2
523,606 - btuC MCAODC_18310 0.46 +0.6
523,620 - btuC MCAODC_18310 0.48 +0.2
523,645 + btuC MCAODC_18310 0.50 -0.4
523,743 - btuC MCAODC_18310 0.60 -0.4
523,756 + btuC MCAODC_18310 0.61 +1.6
523,866 - btuC MCAODC_18310 0.73 +1.6
524,048 + +1.6

Or see this region's nucleotide sequence