Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pspD and pspE are separated by 74 nucleotides pspE and MCAODC_15885 are separated by 209 nucleotides MCAODC_15885 and ycjM overlap by 1 nucleotides ycjM and ycjN are separated by 13 nucleotides ycjN and MCAODC_15900 are separated by 48 nucleotides
MCAODC_15875: pspD - phage shock protein PspD, at 67,200 to 67,421
pspD
MCAODC_15880: pspE - thiosulfate sulfurtransferase PspE, at 67,496 to 67,810
pspE
MCAODC_15885: MCAODC_15885 - Alpha-amylase, at 68,020 to 68,394
_15885
MCAODC_15890: ycjM - Glucosylglycerate phosphorylase, at 68,394 to 69,698
ycjM
MCAODC_15895: ycjN - Putative ABC transporter periplasmic-binding protein YcjN, at 69,712 to 70,341
ycjN
MCAODC_15900: MCAODC_15900 - ABC transporter substrate-binding protein, at 70,390 to 71,004
_15900
Position (kb) 68
69
70 Strain fitness (log2 ratio) -2
-1
0
1 at 67.456 kb on + strand at 67.459 kb on - strand at 67.481 kb on + strand at 67.622 kb on - strand, within pspE at 67.692 kb on - strand, within pspE at 67.692 kb on - strand, within pspE at 67.750 kb on + strand, within pspE at 67.750 kb on + strand, within pspE at 67.866 kb on - strand at 67.876 kb on - strand at 67.880 kb on + strand at 67.881 kb on - strand at 68.071 kb on - strand, within MCAODC_15885 at 68.691 kb on + strand, within ycjM at 68.823 kb on - strand, within ycjM at 68.891 kb on + strand, within ycjM at 69.167 kb on - strand, within ycjM at 69.283 kb on - strand, within ycjM at 69.598 kb on + strand at 69.797 kb on + strand, within ycjN at 69.994 kb on + strand, within ycjN at 70.088 kb on + strand, within ycjN at 70.340 kb on - strand at 70.505 kb on - strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.514 kb on - strand, within MCAODC_15900 at 70.514 kb on - strand, within MCAODC_15900 at 70.515 kb on + strand, within MCAODC_15900
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 67,456 + +1.0 67,459 - -1.6 67,481 + -2.7 67,622 - pspE MCAODC_15880 0.40 -0.8 67,692 - pspE MCAODC_15880 0.62 +0.6 67,692 - pspE MCAODC_15880 0.62 +0.5 67,750 + pspE MCAODC_15880 0.81 -0.8 67,750 + pspE MCAODC_15880 0.81 +1.4 67,866 - -0.7 67,876 - -0.0 67,880 + +1.4 67,881 - +0.3 68,071 - MCAODC_15885 0.14 -0.8 68,691 + ycjM MCAODC_15890 0.23 -0.3 68,823 - ycjM MCAODC_15890 0.33 -0.3 68,891 + ycjM MCAODC_15890 0.38 -1.0 69,167 - ycjM MCAODC_15890 0.59 +1.6 69,283 - ycjM MCAODC_15890 0.68 +0.5 69,598 + +0.2 69,797 + ycjN MCAODC_15895 0.13 +0.6 69,994 + ycjN MCAODC_15895 0.45 +0.8 70,088 + ycjN MCAODC_15895 0.60 +1.6 70,340 - +0.5 70,505 - MCAODC_15900 0.19 -0.1 70,513 + MCAODC_15900 0.20 +1.3 70,513 + MCAODC_15900 0.20 -1.4 70,513 + MCAODC_15900 0.20 +0.7 70,513 + MCAODC_15900 0.20 +0.2 70,513 + MCAODC_15900 0.20 -0.9 70,513 + MCAODC_15900 0.20 +1.6 70,513 + MCAODC_15900 0.20 +0.6 70,514 - MCAODC_15900 0.20 -0.4 70,514 - MCAODC_15900 0.20 -1.6 70,515 + MCAODC_15900 0.20 +1.1
Or see this region's nucleotide sequence