Strain Fitness in Escherichia coli ECRC101 around MCAODC_15695

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntacnA and ribA are separated by 63 nucleotidesribA and pgpB are separated by 169 nucleotides MCAODC_15690: acnA - aconitate hydratase AcnA, at 26,525 to 29,200 acnA MCAODC_15695: ribA - GTP cyclohydrolase-2, at 29,264 to 29,854 ribA MCAODC_15700: pgpB - phosphatidylglycerophosphatase B, at 30,024 to 30,788 pgpB Position (kb) 29 30Strain fitness (log2 ratio) -2 -1 0 1 2at 28.271 kb on + strand, within acnAat 28.415 kb on + strand, within acnAat 28.440 kb on - strand, within acnAat 28.441 kb on + strand, within acnAat 28.499 kb on - strand, within acnAat 28.552 kb on + strand, within acnAat 28.552 kb on + strand, within acnAat 28.570 kb on + strand, within acnAat 28.599 kb on - strand, within acnAat 28.602 kb on + strand, within acnAat 28.602 kb on + strand, within acnAat 28.602 kb on + strand, within acnAat 28.658 kb on - strand, within acnAat 28.749 kb on + strand, within acnAat 28.764 kb on + strand, within acnAat 28.793 kb on + strand, within acnAat 28.795 kb on + strand, within acnAat 28.795 kb on + strand, within acnAat 28.838 kb on + strand, within acnAat 29.147 kb on + strandat 29.206 kb on - strandat 29.266 kb on - strandat 29.869 kb on - strandat 29.920 kb on - strandat 30.074 kb on + strandat 30.136 kb on + strand, within pgpBat 30.257 kb on - strand, within pgpBat 30.299 kb on + strand, within pgpBat 30.394 kb on + strand, within pgpBat 30.459 kb on + strand, within pgpBat 30.587 kb on + strand, within pgpBat 30.664 kb on + strand, within pgpBat 30.668 kb on + strand, within pgpBat 30.668 kb on + strand, within pgpBat 30.668 kb on + strand, within pgpBat 30.669 kb on - strand, within pgpBat 30.734 kb on - strandat 30.763 kb on + strandat 30.763 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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28,271 + acnA MCAODC_15690 0.65 -0.7
28,415 + acnA MCAODC_15690 0.71 +0.1
28,440 - acnA MCAODC_15690 0.72 -0.9
28,441 + acnA MCAODC_15690 0.72 +2.0
28,499 - acnA MCAODC_15690 0.74 +0.1
28,552 + acnA MCAODC_15690 0.76 +1.1
28,552 + acnA MCAODC_15690 0.76 +1.0
28,570 + acnA MCAODC_15690 0.76 +1.0
28,599 - acnA MCAODC_15690 0.78 +0.3
28,602 + acnA MCAODC_15690 0.78 +1.6
28,602 + acnA MCAODC_15690 0.78 -1.0
28,602 + acnA MCAODC_15690 0.78 +0.2
28,658 - acnA MCAODC_15690 0.80 +0.6
28,749 + acnA MCAODC_15690 0.83 -2.0
28,764 + acnA MCAODC_15690 0.84 +0.6
28,793 + acnA MCAODC_15690 0.85 +1.0
28,795 + acnA MCAODC_15690 0.85 -0.0
28,795 + acnA MCAODC_15690 0.85 -0.0
28,838 + acnA MCAODC_15690 0.86 +1.3
29,147 + -0.2
29,206 - +1.6
29,266 - +1.0
29,869 - +1.3
29,920 - -1.8
30,074 + +0.4
30,136 + pgpB MCAODC_15700 0.15 +0.6
30,257 - pgpB MCAODC_15700 0.30 -0.8
30,299 + pgpB MCAODC_15700 0.36 -0.4
30,394 + pgpB MCAODC_15700 0.48 +1.6
30,459 + pgpB MCAODC_15700 0.57 +1.0
30,587 + pgpB MCAODC_15700 0.74 +0.2
30,664 + pgpB MCAODC_15700 0.84 +0.6
30,668 + pgpB MCAODC_15700 0.84 -1.4
30,668 + pgpB MCAODC_15700 0.84 -0.6
30,668 + pgpB MCAODC_15700 0.84 +0.6
30,669 - pgpB MCAODC_15700 0.84 +0.3
30,734 - -0.0
30,763 + +0.8
30,763 + +1.0

Or see this region's nucleotide sequence