Strain Fitness in Escherichia coli ECRC101 around MCAODC_10270

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaraC and sctC overlap by 4 nucleotidessctC and MCAODC_10275 are separated by 4 nucleotides MCAODC_10265: araC - AraC family transcriptional regulator, at 2,049,914 to 2,050,663 araC MCAODC_10270: sctC - type III secretion system outer membrane ring subunit SctC, at 2,050,660 to 2,052,363 sctC MCAODC_10275: MCAODC_10275 - YopN/LcrE/InvE/MxiC type III secretion system gatekeeper, at 2,052,368 to 2,053,513 _10275 Position (kb) 2050 2051 2052 2053Strain fitness (log2 ratio) -1 0 1at 2049.713 kb on + strandat 2049.790 kb on + strandat 2049.933 kb on + strandat 2050.177 kb on + strand, within araCat 2050.465 kb on + strand, within araCat 2050.565 kb on - strand, within araCat 2050.763 kb on + strandat 2050.824 kb on + strandat 2050.829 kb on + strandat 2050.829 kb on + strandat 2050.926 kb on + strand, within sctCat 2050.955 kb on - strand, within sctCat 2050.960 kb on + strand, within sctCat 2050.961 kb on - strand, within sctCat 2051.096 kb on - strand, within sctCat 2051.100 kb on - strand, within sctCat 2051.116 kb on + strand, within sctCat 2051.117 kb on - strand, within sctCat 2051.199 kb on - strand, within sctCat 2051.258 kb on + strand, within sctCat 2051.301 kb on + strand, within sctCat 2051.310 kb on + strand, within sctCat 2051.373 kb on - strand, within sctCat 2051.631 kb on - strand, within sctCat 2051.883 kb on - strand, within sctCat 2051.887 kb on + strand, within sctCat 2051.888 kb on - strand, within sctCat 2052.027 kb on - strand, within sctCat 2052.027 kb on - strand, within sctCat 2052.027 kb on - strand, within sctCat 2052.031 kb on - strand, within sctCat 2052.078 kb on + strand, within sctCat 2052.078 kb on + strand, within sctCat 2052.079 kb on - strand, within sctCat 2052.436 kb on + strandat 2052.436 kb on + strandat 2052.499 kb on + strand, within MCAODC_10275at 2052.523 kb on + strand, within MCAODC_10275at 2052.524 kb on - strand, within MCAODC_10275at 2052.524 kb on - strand, within MCAODC_10275at 2052.677 kb on - strand, within MCAODC_10275at 2052.808 kb on - strand, within MCAODC_10275

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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2,049,713 + -0.4
2,049,790 + +0.2
2,049,933 + +0.2
2,050,177 + araC MCAODC_10265 0.35 -0.5
2,050,465 + araC MCAODC_10265 0.73 -0.4
2,050,565 - araC MCAODC_10265 0.87 -0.4
2,050,763 + +0.3
2,050,824 + -1.4
2,050,829 + -1.1
2,050,829 + +1.5
2,050,926 + sctC MCAODC_10270 0.16 +0.0
2,050,955 - sctC MCAODC_10270 0.17 +0.6
2,050,960 + sctC MCAODC_10270 0.18 +0.7
2,050,961 - sctC MCAODC_10270 0.18 +0.2
2,051,096 - sctC MCAODC_10270 0.26 +1.0
2,051,100 - sctC MCAODC_10270 0.26 +0.7
2,051,116 + sctC MCAODC_10270 0.27 +0.9
2,051,117 - sctC MCAODC_10270 0.27 -0.4
2,051,199 - sctC MCAODC_10270 0.32 +1.0
2,051,258 + sctC MCAODC_10270 0.35 +0.7
2,051,301 + sctC MCAODC_10270 0.38 -1.7
2,051,310 + sctC MCAODC_10270 0.38 +0.5
2,051,373 - sctC MCAODC_10270 0.42 +1.7
2,051,631 - sctC MCAODC_10270 0.57 +0.6
2,051,883 - sctC MCAODC_10270 0.72 +0.2
2,051,887 + sctC MCAODC_10270 0.72 +0.2
2,051,888 - sctC MCAODC_10270 0.72 +0.2
2,052,027 - sctC MCAODC_10270 0.80 -0.4
2,052,027 - sctC MCAODC_10270 0.80 -0.7
2,052,027 - sctC MCAODC_10270 0.80 -1.3
2,052,031 - sctC MCAODC_10270 0.80 +1.7
2,052,078 + sctC MCAODC_10270 0.83 +0.4
2,052,078 + sctC MCAODC_10270 0.83 -0.7
2,052,079 - sctC MCAODC_10270 0.83 +0.2
2,052,436 + +0.7
2,052,436 + -1.5
2,052,499 + MCAODC_10275 0.11 +0.6
2,052,523 + MCAODC_10275 0.14 -0.2
2,052,524 - MCAODC_10275 0.14 +1.0
2,052,524 - MCAODC_10275 0.14 +0.9
2,052,677 - MCAODC_10275 0.27 +0.2
2,052,808 - MCAODC_10275 0.38 +1.0

Or see this region's nucleotide sequence