Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nfeF and Ile2_trna are separated by 53 nucleotides Ile2_trna and mug are separated by 124 nucleotides mug and rpoD are separated by 78 nucleotides rpoD and dnaG are separated by 194 nucleotides
MCAODC_09155: nfeF - NADPH-dependent ferric chelate reductase, at 1,828,744 to 1,829,508
nfeF
MCAODC_09160: Ile2_trna - tRNA-Ile2(cat), at 1,829,562 to 1,829,637
_trna
MCAODC_09165: mug - G/U mismatch-specific DNA glycosylase, at 1,829,762 to 1,830,268
mug
MCAODC_09170: rpoD - RNA polymerase sigma factor RpoD, at 1,830,347 to 1,832,188
rpoD
MCAODC_09175: dnaG - DNA primase, at 1,832,383 to 1,834,128
dnaG
Position (kb)
1830
1831
1832
1833 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1829.392 kb on + strand, within nfeF at 1829.393 kb on - strand, within nfeF at 1829.393 kb on - strand, within nfeF at 1829.418 kb on + strand, within nfeF at 1829.419 kb on - strand, within nfeF at 1829.492 kb on - strand at 1829.515 kb on + strand at 1829.556 kb on + strand at 1829.556 kb on + strand at 1829.556 kb on + strand at 1829.557 kb on - strand at 1829.557 kb on - strand at 1829.558 kb on + strand at 1829.559 kb on - strand at 1829.559 kb on - strand at 1829.602 kb on - strand, within Ile2_trna at 1829.629 kb on + strand, within Ile2_trna at 1829.650 kb on + strand at 1829.652 kb on + strand at 1829.657 kb on + strand at 1829.682 kb on + strand at 1829.730 kb on - strand at 1829.730 kb on - strand at 1829.747 kb on + strand at 1829.748 kb on - strand at 1829.757 kb on + strand at 1829.757 kb on + strand at 1829.758 kb on - strand at 1829.761 kb on + strand at 1829.761 kb on + strand at 1829.883 kb on + strand, within mug at 1829.885 kb on + strand, within mug at 1829.885 kb on + strand, within mug at 1829.885 kb on + strand, within mug at 1829.886 kb on - strand, within mug at 1829.899 kb on + strand, within mug at 1829.945 kb on + strand, within mug at 1830.095 kb on + strand, within mug at 1830.095 kb on + strand, within mug at 1830.095 kb on + strand, within mug at 1830.193 kb on - strand, within mug at 1830.324 kb on + strand at 1832.306 kb on - strand at 1832.310 kb on - strand at 1832.310 kb on - strand at 1832.310 kb on - strand at 1832.310 kb on - strand at 1832.310 kb on - strand at 1832.310 kb on - strand at 1832.312 kb on - strand at 1832.312 kb on - strand at 1832.314 kb on - strand at 1832.314 kb on - strand at 1832.314 kb on - strand at 1832.372 kb on - strand at 1832.372 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 1,829,392 + nfeF MCAODC_09155 0.85 +0.1 1,829,393 - nfeF MCAODC_09155 0.85 -0.8 1,829,393 - nfeF MCAODC_09155 0.85 -1.1 1,829,418 + nfeF MCAODC_09155 0.88 -0.5 1,829,419 - nfeF MCAODC_09155 0.88 -0.0 1,829,492 - -1.8 1,829,515 + +0.7 1,829,556 + +1.6 1,829,556 + +0.5 1,829,556 + +1.5 1,829,557 - +0.7 1,829,557 - +0.0 1,829,558 + -0.1 1,829,559 - -2.0 1,829,559 - +0.6 1,829,602 - Ile2_trna MCAODC_09160 0.53 -0.8 1,829,629 + Ile2_trna MCAODC_09160 0.88 -1.1 1,829,650 + -1.4 1,829,652 + -0.8 1,829,657 + -2.3 1,829,682 + -0.4 1,829,730 - -0.7 1,829,730 - +0.6 1,829,747 + +0.1 1,829,748 - +0.8 1,829,757 + +0.1 1,829,757 + +0.7 1,829,758 - -0.7 1,829,761 + -0.5 1,829,761 + +1.0 1,829,883 + mug MCAODC_09165 0.24 +0.4 1,829,885 + mug MCAODC_09165 0.24 -0.2 1,829,885 + mug MCAODC_09165 0.24 +1.1 1,829,885 + mug MCAODC_09165 0.24 -0.5 1,829,886 - mug MCAODC_09165 0.24 +0.7 1,829,899 + mug MCAODC_09165 0.27 +0.2 1,829,945 + mug MCAODC_09165 0.36 +0.6 1,830,095 + mug MCAODC_09165 0.66 -0.7 1,830,095 + mug MCAODC_09165 0.66 -1.9 1,830,095 + mug MCAODC_09165 0.66 +0.1 1,830,193 - mug MCAODC_09165 0.85 -0.8 1,830,324 + -0.9 1,832,306 - +1.8 1,832,310 - -0.8 1,832,310 - +0.8 1,832,310 - +0.2 1,832,310 - +1.8 1,832,310 - -1.0 1,832,310 - -0.5 1,832,312 - +2.8 1,832,312 - +0.6 1,832,314 - -0.8 1,832,314 - -0.2 1,832,314 - -0.2 1,832,372 - -0.7 1,832,372 - -1.7
Or see this region's nucleotide sequence