Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaG and apaH are separated by 6 nucleotides apaH and MCAODC_01365 overlap by 4 nucleotides MCAODC_01365 and MCAODC_01370 are separated by 2 nucleotides MCAODC_01370 and MCAODC_01375 are separated by 85 nucleotides MCAODC_01375 and kefC are separated by 80 nucleotides
MCAODC_01355: apaG - Co2+/Mg2+ efflux protein ApaG, at 260,614 to 260,991
apaG
MCAODC_01360: apaH - bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH, at 260,998 to 261,846
apaH
MCAODC_01365: MCAODC_01365 - plasmid maintenance protein CcdB, at 261,843 to 262,157
_01365
MCAODC_01370: MCAODC_01370 - antitoxin, at 262,160 to 262,393
_01370
MCAODC_01375: MCAODC_01375 - Dihydrofolate reductase, at 262,479 to 263,069
_01375
MCAODC_01380: kefC - glutathione-regulated potassium-efflux system protein KefC, at 263,150 to 265,012
kefC
Position (kb) 261
262
263 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 260.857 kb on + strand, within apaG at 260.858 kb on - strand, within apaG at 261.007 kb on + strand at 261.007 kb on + strand at 261.008 kb on - strand at 261.008 kb on - strand at 261.173 kb on + strand, within apaH at 261.173 kb on + strand, within apaH at 261.174 kb on - strand, within apaH at 261.174 kb on - strand, within apaH at 261.580 kb on + strand, within apaH at 261.868 kb on - strand at 261.868 kb on - strand at 261.868 kb on - strand at 261.878 kb on + strand, within MCAODC_01365 at 261.879 kb on - strand, within MCAODC_01365 at 261.967 kb on + strand, within MCAODC_01365 at 261.967 kb on + strand, within MCAODC_01365 at 261.967 kb on + strand, within MCAODC_01365 at 261.968 kb on - strand, within MCAODC_01365 at 261.968 kb on - strand, within MCAODC_01365 at 261.988 kb on - strand, within MCAODC_01365 at 262.040 kb on + strand, within MCAODC_01365 at 262.041 kb on - strand, within MCAODC_01365 at 262.041 kb on - strand, within MCAODC_01365 at 262.041 kb on - strand, within MCAODC_01365 at 262.082 kb on + strand, within MCAODC_01365 at 262.082 kb on + strand, within MCAODC_01365 at 262.142 kb on - strand at 262.142 kb on - strand at 262.148 kb on + strand at 262.149 kb on - strand at 262.161 kb on + strand at 262.225 kb on + strand, within MCAODC_01370 at 262.310 kb on - strand, within MCAODC_01370 at 262.313 kb on + strand, within MCAODC_01370 at 262.314 kb on - strand, within MCAODC_01370 at 262.314 kb on - strand, within MCAODC_01370 at 262.341 kb on - strand, within MCAODC_01370 at 262.419 kb on - strand at 262.424 kb on + strand at 262.427 kb on + strand at 262.428 kb on - strand at 262.448 kb on - strand at 262.448 kb on - strand at 262.448 kb on - strand at 262.467 kb on + strand at 262.467 kb on + strand at 262.468 kb on - strand at 262.468 kb on - strand at 262.470 kb on + strand at 262.472 kb on + strand at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.994 kb on - strand, within MCAODC_01375 at 262.997 kb on + strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 262.998 kb on - strand, within MCAODC_01375 at 263.048 kb on + strand at 263.050 kb on + strand at 263.118 kb on + strand at 263.118 kb on + strand at 263.119 kb on - strand at 263.125 kb on - strand at 263.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 260,857 + apaG MCAODC_01355 0.64 +0.4 260,858 - apaG MCAODC_01355 0.65 +2.8 261,007 + +0.0 261,007 + +0.8 261,008 - -0.2 261,008 - +0.5 261,173 + apaH MCAODC_01360 0.21 +1.8 261,173 + apaH MCAODC_01360 0.21 +0.2 261,174 - apaH MCAODC_01360 0.21 +1.8 261,174 - apaH MCAODC_01360 0.21 +0.2 261,580 + apaH MCAODC_01360 0.69 +1.2 261,868 - -0.0 261,868 - -0.8 261,868 - +1.8 261,878 + MCAODC_01365 0.11 +2.6 261,879 - MCAODC_01365 0.11 +0.2 261,967 + MCAODC_01365 0.39 -0.3 261,967 + MCAODC_01365 0.39 -1.4 261,967 + MCAODC_01365 0.39 -0.6 261,968 - MCAODC_01365 0.40 -2.3 261,968 - MCAODC_01365 0.40 -1.3 261,988 - MCAODC_01365 0.46 +1.1 262,040 + MCAODC_01365 0.63 -0.9 262,041 - MCAODC_01365 0.63 +0.8 262,041 - MCAODC_01365 0.63 +0.1 262,041 - MCAODC_01365 0.63 -1.0 262,082 + MCAODC_01365 0.76 +0.2 262,082 + MCAODC_01365 0.76 +0.8 262,142 - -1.2 262,142 - +0.1 262,148 + +0.3 262,149 - +0.1 262,161 + +0.4 262,225 + MCAODC_01370 0.28 +1.2 262,310 - MCAODC_01370 0.64 -0.3 262,313 + MCAODC_01370 0.65 -0.3 262,314 - MCAODC_01370 0.66 -0.8 262,314 - MCAODC_01370 0.66 +1.6 262,341 - MCAODC_01370 0.77 -1.2 262,419 - +1.1 262,424 + -0.3 262,427 + +0.8 262,428 - +0.0 262,448 - +0.7 262,448 - +1.8 262,448 - -0.3 262,467 + -1.5 262,467 + -0.7 262,468 - -0.3 262,468 - +1.8 262,470 + +2.6 262,472 + +1.2 262,994 - MCAODC_01375 0.87 -1.6 262,994 - MCAODC_01375 0.87 -3.0 262,994 - MCAODC_01375 0.87 +0.8 262,994 - MCAODC_01375 0.87 +1.1 262,994 - MCAODC_01375 0.87 -0.6 262,997 + MCAODC_01375 0.88 +1.8 262,998 - MCAODC_01375 0.88 -0.6 262,998 - MCAODC_01375 0.88 -0.4 262,998 - MCAODC_01375 0.88 -0.7 262,998 - MCAODC_01375 0.88 -0.8 262,998 - MCAODC_01375 0.88 -0.1 262,998 - MCAODC_01375 0.88 -1.2 263,048 + +0.4 263,050 + +0.2 263,118 + -0.4 263,118 + -0.5 263,119 - +2.6 263,125 - -0.4 263,151 + -0.2
Or see this region's nucleotide sequence