Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF483

Experiment: Ying_all64 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF481 and GFF482 overlap by 4 nucleotidesGFF482 and GFF483 are separated by 5 nucleotidesGFF483 and GFF484 overlap by 4 nucleotides GFF481 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4), at 145,459 to 146,631 GFF481 GFF482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 146,628 to 147,428 GFF482 GFF483 - Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4), at 147,434 to 149,341 GFF483 GFF484 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2), at 149,338 to 150,501 GFF484 Position (kb) 147 148 149 150Strain fitness (log2 ratio) -1 0 1at 146.736 kb on + strand, within GFF482at 148.224 kb on - strand, within GFF483at 149.795 kb on - strand, within GFF484

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 1
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146,736 + GFF482 0.13 +0.2
148,224 - GFF483 0.41 -0.4
149,795 - GFF484 0.39 +0.3

Or see this region's nucleotide sequence