Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF417

Experiment: Ying_all64 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF415 and GFF416 are separated by 2 nucleotidesGFF416 and GFF417 are separated by 7 nucleotidesGFF417 and GFF418 are separated by 193 nucleotidesGFF418 and GFF419 are separated by 19 nucleotides GFF415 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 85,203 to 86,411 GFF415 GFF416 - TesB-like acyl-CoA thioesterase 2, at 86,414 to 87,229 GFF416 GFF417 - putative patatin-like phospholipase, at 87,237 to 89,507 GFF417 GFF418 - Enoyl-CoA hydratase (EC 4.2.1.17), at 89,701 to 90,465 GFF418 GFF419 - hypothetical protein, at 90,485 to 90,859 GFF419 Position (kb) 87 88 89 90Strain fitness (log2 ratio) -2 -1 0 1 2at 86.347 kb on - strandat 86.347 kb on - strandat 86.347 kb on - strandat 86.466 kb on - strandat 86.598 kb on - strand, within GFF416at 86.725 kb on + strand, within GFF416at 86.856 kb on - strand, within GFF416at 86.856 kb on - strand, within GFF416at 86.856 kb on - strand, within GFF416at 86.856 kb on - strand, within GFF416at 86.996 kb on + strand, within GFF416at 86.996 kb on + strand, within GFF416at 87.059 kb on + strand, within GFF416at 87.059 kb on + strand, within GFF416at 87.060 kb on - strand, within GFF416at 87.060 kb on - strand, within GFF416at 87.219 kb on - strandat 87.219 kb on - strandat 87.259 kb on + strandat 87.259 kb on + strandat 87.259 kb on + strandat 87.260 kb on - strandat 87.260 kb on - strandat 87.260 kb on - strandat 87.271 kb on + strandat 87.301 kb on + strandat 87.464 kb on - strand, within GFF417at 87.466 kb on + strand, within GFF417at 87.466 kb on + strand, within GFF417at 87.466 kb on + strand, within GFF417at 87.466 kb on + strand, within GFF417at 87.466 kb on + strand, within GFF417at 87.467 kb on - strand, within GFF417at 87.467 kb on - strand, within GFF417at 87.467 kb on - strand, within GFF417at 87.467 kb on - strand, within GFF417at 87.467 kb on - strand, within GFF417at 87.467 kb on - strand, within GFF417at 87.490 kb on + strand, within GFF417at 87.490 kb on + strand, within GFF417at 87.490 kb on + strand, within GFF417at 87.491 kb on - strand, within GFF417at 87.491 kb on - strand, within GFF417at 87.491 kb on - strand, within GFF417at 87.491 kb on - strand, within GFF417at 87.550 kb on + strand, within GFF417at 87.751 kb on + strand, within GFF417at 87.751 kb on + strand, within GFF417at 87.752 kb on - strand, within GFF417at 88.022 kb on - strand, within GFF417at 88.063 kb on + strand, within GFF417at 88.063 kb on + strand, within GFF417at 88.063 kb on + strand, within GFF417at 88.063 kb on + strand, within GFF417at 88.063 kb on + strand, within GFF417at 88.063 kb on + strand, within GFF417at 88.075 kb on + strand, within GFF417at 88.075 kb on + strand, within GFF417at 88.219 kb on + strand, within GFF417at 88.219 kb on + strand, within GFF417at 88.220 kb on - strand, within GFF417at 88.295 kb on - strand, within GFF417at 88.295 kb on - strand, within GFF417at 88.295 kb on - strand, within GFF417at 89.032 kb on + strand, within GFF417at 89.254 kb on + strand, within GFF417at 89.254 kb on + strand, within GFF417at 89.255 kb on - strand, within GFF417at 89.493 kb on - strandat 89.791 kb on + strand, within GFF418at 89.791 kb on + strand, within GFF418at 89.792 kb on - strand, within GFF418at 89.792 kb on - strand, within GFF418at 89.792 kb on - strand, within GFF418at 90.049 kb on + strand, within GFF418at 90.145 kb on + strand, within GFF418at 90.486 kb on + strandat 90.486 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 1
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86,347 - -0.6
86,347 - +0.1
86,347 - +0.1
86,466 - +0.5
86,598 - GFF416 0.23 +0.3
86,725 + GFF416 0.38 +2.0
86,856 - GFF416 0.54 +0.4
86,856 - GFF416 0.54 +0.9
86,856 - GFF416 0.54 -0.2
86,856 - GFF416 0.54 +1.4
86,996 + GFF416 0.71 +0.3
86,996 + GFF416 0.71 -0.6
87,059 + GFF416 0.79 -0.0
87,059 + GFF416 0.79 -0.1
87,060 - GFF416 0.79 +0.4
87,060 - GFF416 0.79 +0.3
87,219 - -0.2
87,219 - +0.7
87,259 + -1.9
87,259 + -0.6
87,259 + -0.8
87,260 - +0.4
87,260 - +0.7
87,260 - +0.3
87,271 + +0.0
87,301 + +0.4
87,464 - GFF417 0.10 +0.1
87,466 + GFF417 0.10 -0.9
87,466 + GFF417 0.10 +0.5
87,466 + GFF417 0.10 -0.6
87,466 + GFF417 0.10 +0.3
87,466 + GFF417 0.10 +0.4
87,467 - GFF417 0.10 -0.8
87,467 - GFF417 0.10 +0.4
87,467 - GFF417 0.10 -0.9
87,467 - GFF417 0.10 +0.6
87,467 - GFF417 0.10 -0.6
87,467 - GFF417 0.10 -1.7
87,490 + GFF417 0.11 -0.3
87,490 + GFF417 0.11 -1.3
87,490 + GFF417 0.11 +0.7
87,491 - GFF417 0.11 -0.4
87,491 - GFF417 0.11 -0.1
87,491 - GFF417 0.11 -0.2
87,491 - GFF417 0.11 +0.2
87,550 + GFF417 0.14 +1.2
87,751 + GFF417 0.23 -0.2
87,751 + GFF417 0.23 -0.1
87,752 - GFF417 0.23 -0.4
88,022 - GFF417 0.35 -0.6
88,063 + GFF417 0.36 -0.3
88,063 + GFF417 0.36 -0.6
88,063 + GFF417 0.36 -0.3
88,063 + GFF417 0.36 -0.2
88,063 + GFF417 0.36 -0.5
88,063 + GFF417 0.36 +0.1
88,075 + GFF417 0.37 -0.1
88,075 + GFF417 0.37 -0.4
88,219 + GFF417 0.43 -0.1
88,219 + GFF417 0.43 +0.3
88,220 - GFF417 0.43 -0.6
88,295 - GFF417 0.47 +0.3
88,295 - GFF417 0.47 -2.2
88,295 - GFF417 0.47 -0.7
89,032 + GFF417 0.79 +0.4
89,254 + GFF417 0.89 +0.8
89,254 + GFF417 0.89 +0.1
89,255 - GFF417 0.89 -0.6
89,493 - -1.6
89,791 + GFF418 0.12 +1.0
89,791 + GFF418 0.12 -0.7
89,792 - GFF418 0.12 -0.6
89,792 - GFF418 0.12 +0.2
89,792 - GFF418 0.12 -0.2
90,049 + GFF418 0.45 +0.7
90,145 + GFF418 0.58 -0.2
90,486 + -0.0
90,486 + +0.5

Or see this region's nucleotide sequence