Strain Fitness in Hydrogenophaga sp. GW460-11-11-14-LB1 around GFF2216

Experiment: Ying_all64 rep A; time point 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2214 and GFF2215 overlap by 4 nucleotidesGFF2215 and GFF2216 overlap by 10 nucleotidesGFF2216 and GFF2217 overlap by 4 nucleotidesGFF2217 and GFF2218 are separated by 26 nucleotides GFF2214 - [NiFe] hydrogenase metallocenter assembly protein HypD, at 1,103,994 to 1,105,154 GFF2214 GFF2215 - [NiFe] hydrogenase metallocenter assembly protein HypC, at 1,105,151 to 1,105,387 GFF2215 GFF2216 - [NiFe] hydrogenase metallocenter assembly protein HypF, at 1,105,378 to 1,106,553 GFF2216 GFF2217 - [NiFe] hydrogenase nickel incorporation-associated protein HypB, at 1,106,550 to 1,107,410 GFF2217 GFF2218 - [NiFe] hydrogenase nickel incorporation protein HypA, at 1,107,437 to 1,107,784 GFF2218 Position (kb) 1105 1106 1107Strain fitness (log2 ratio) -1 0 1at 1104.517 kb on + strand, within GFF2214at 1104.799 kb on + strand, within GFF2214at 1104.884 kb on - strand, within GFF2214at 1105.067 kb on - strandat 1105.263 kb on + strand, within GFF2215at 1105.379 kb on + strandat 1105.380 kb on - strandat 1105.380 kb on - strandat 1106.774 kb on - strand, within GFF2217at 1107.399 kb on + strandat 1107.400 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 1
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1,104,517 + GFF2214 0.45 -0.1
1,104,799 + GFF2214 0.69 +0.1
1,104,884 - GFF2214 0.77 +0.2
1,105,067 - +0.5
1,105,263 + GFF2215 0.47 -0.2
1,105,379 + +0.0
1,105,380 - +0.4
1,105,380 - +0.1
1,106,774 - GFF2217 0.26 +0.5
1,107,399 + +0.3
1,107,400 - +1.5

Or see this region's nucleotide sequence