Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28745

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_28735 and hipB are separated by 263 nucleotideshipB and OKFHMN_28745 are separated by 76 nucleotidesOKFHMN_28745 and OKFHMN_28750 overlap by 17 nucleotidesOKFHMN_28750 and eCs1768 overlap by 29 nucleotides OKFHMN_28735: OKFHMN_28735 - hypothetical protein, at 5,512,018 to 5,512,317 _28735 OKFHMN_28740: hipB - transcriptional regulator, at 5,512,581 to 5,512,988 hipB OKFHMN_28745: OKFHMN_28745 - transcriptional regulator, at 5,513,065 to 5,513,292 _28745 OKFHMN_28750: OKFHMN_28750 - Putative bacterial toxin ydaT, at 5,513,276 to 5,513,827 _28750 OKFHMN_28755: eCs1768 - DNA-binding protein, at 5,513,799 to 5,514,839 eCs1768 Position (kb) 5513 5514Strain fitness (log2 ratio) -1 0 1at 5512.269 kb on + strand, within OKFHMN_28735at 5512.305 kb on + strandat 5512.308 kb on - strandat 5512.583 kb on - strandat 5513.248 kb on - strand, within OKFHMN_28745at 5513.971 kb on + strand, within eCs1768at 5514.045 kb on + strand, within eCs1768

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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5,512,269 + OKFHMN_28735 0.84 +1.2
5,512,305 + +0.2
5,512,308 - -0.6
5,512,583 - -0.5
5,513,248 - OKFHMN_28745 0.80 -0.3
5,513,971 + eCs1768 OKFHMN_28755 0.17 +1.2
5,514,045 + eCs1768 OKFHMN_28755 0.24 -0.2

Or see this region's nucleotide sequence