Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28175

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_28170 and menH are separated by 302 nucleotidesmenH and ompT are separated by 186 nucleotides OKFHMN_28170: OKFHMN_28170 - IS66-like element ISEc8 family transposase, at 5,404,966 to 5,406,504 _28170 OKFHMN_28175: menH - alpha/beta hydrolase, at 5,406,807 to 5,408,291 menH OKFHMN_28180: ompT - omptin family outer membrane protease OmpT, at 5,408,478 to 5,409,431 ompT Position (kb) 5406 5407 5408 5409Strain fitness (log2 ratio) -2 -1 0 1at 5407.013 kb on - strand, within menHat 5407.440 kb on + strand, within menHat 5408.232 kb on + strandat 5408.586 kb on - strand, within ompTat 5408.928 kb on - strand, within ompTat 5408.954 kb on + strand, within ompTat 5408.955 kb on - strand, within ompTat 5409.132 kb on - strand, within ompTat 5409.219 kb on - strand, within ompT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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5,407,013 - menH OKFHMN_28175 0.14 -1.1
5,407,440 + menH OKFHMN_28175 0.43 -0.2
5,408,232 + -0.8
5,408,586 - ompT OKFHMN_28180 0.11 +0.0
5,408,928 - ompT OKFHMN_28180 0.47 +0.6
5,408,954 + ompT OKFHMN_28180 0.50 -1.8
5,408,955 - ompT OKFHMN_28180 0.50 -0.2
5,409,132 - ompT OKFHMN_28180 0.69 +0.5
5,409,219 - ompT OKFHMN_28180 0.78 -0.1

Or see this region's nucleotide sequence