Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysE and gpA1 are separated by 43 nucleotides gpA1 and tnp-IS629 are separated by 170 nucleotides tnp-IS629 and tnp-IS3 overlap by 1 nucleotides tnp-IS3 and OKFHMN_25915 are separated by 19 nucleotides OKFHMN_25915 and OKFHMN_25920 are separated by 4 nucleotides
OKFHMN_25890: cysE - serine acetyltransferase, at 5,003,288 to 5,003,860
cysE
OKFHMN_25895: gpA1 - Phage terminase, large subunit GpA, at 5,003,904 to 5,004,320
gpA1
OKFHMN_25905: tnp-IS629 - IS3 family IS629 transposase, at 5,004,491 to 5,004,817
tnp-IS629
OKFHMN_25910: tnp-IS3 - IS3 family transposase, at 5,004,817 to 5,005,506
tnp-IS3
OKFHMN_25915: OKFHMN_25915 - peptide transporter, at 5,005,526 to 5,005,840
_25915
OKFHMN_25920: OKFHMN_25920 - recombinase, at 5,005,845 to 5,006,804
_25920
Position (kb) 5004
5005
5006 Strain fitness (log2 ratio) -1
0
1 at 5003.822 kb on + strand at 5003.822 kb on + strand at 5003.823 kb on - strand at 5005.497 kb on - strand at 5005.527 kb on + strand at 5005.598 kb on - strand, within OKFHMN_25915 at 5005.605 kb on + strand, within OKFHMN_25915 at 5005.691 kb on + strand, within OKFHMN_25915 at 5005.764 kb on + strand, within OKFHMN_25915 at 5005.864 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 5,003,822 + -1.0 5,003,822 + -0.5 5,003,823 - +1.3 5,005,497 - -0.7 5,005,527 + -0.7 5,005,598 - OKFHMN_25915 0.23 +1.3 5,005,605 + OKFHMN_25915 0.25 +0.3 5,005,691 + OKFHMN_25915 0.52 +0.3 5,005,764 + OKFHMN_25915 0.76 +0.9 5,005,864 + -1.1
Or see this region's nucleotide sequence