Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25170

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeO and Asn_trna are separated by 100 nucleotidesAsn_trna and ycdB are separated by 449 nucleotides OKFHMN_25165: yeeO - toxic metabolite efflux MATE transporter YeeO, at 4,882,000 to 4,883,454 yeeO OKFHMN_25170: Asn_trna - tRNA-Asn(gtt), at 4,883,555 to 4,883,630 _trna OKFHMN_25175: ycdB - putative transcriptional regulatory protein YcdB, at 4,884,080 to 4,884,796 ycdB Position (kb) 4883 4884Strain fitness (log2 ratio) -2 -1 0 1at 4882.575 kb on + strand, within yeeOat 4882.582 kb on - strand, within yeeOat 4882.794 kb on + strand, within yeeOat 4883.098 kb on + strand, within yeeOat 4883.160 kb on + strand, within yeeOat 4883.160 kb on + strand, within yeeOat 4883.229 kb on + strand, within yeeOat 4883.230 kb on - strand, within yeeOat 4883.242 kb on - strand, within yeeOat 4883.452 kb on + strandat 4884.410 kb on - strand, within ycdB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,882,575 + yeeO OKFHMN_25165 0.40 +0.0
4,882,582 - yeeO OKFHMN_25165 0.40 -0.3
4,882,794 + yeeO OKFHMN_25165 0.55 -1.7
4,883,098 + yeeO OKFHMN_25165 0.75 +0.2
4,883,160 + yeeO OKFHMN_25165 0.80 +0.8
4,883,160 + yeeO OKFHMN_25165 0.80 -0.8
4,883,229 + yeeO OKFHMN_25165 0.84 +0.4
4,883,230 - yeeO OKFHMN_25165 0.85 +0.3
4,883,242 - yeeO OKFHMN_25165 0.85 -0.2
4,883,452 + -1.8
4,884,410 - ycdB OKFHMN_25175 0.46 +0.3

Or see this region's nucleotide sequence