Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22100

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteutR and hemF are separated by 5 nucleotideshemF and amiA are separated by 3 nucleotidesamiA and ypeA are separated by 213 nucleotidesypeA and yfeZ overlap by 14 nucleotides OKFHMN_22090: eutR - HTH-type transcriptional regulator EutR, at 4,296,294 to 4,297,346 eutR OKFHMN_22095: hemF - oxygen-dependent coproporphyrinogen oxidase, at 4,297,352 to 4,298,251 hemF OKFHMN_22100: amiA - N-acetylmuramoyl-L-alanine amidase AmiA, at 4,298,255 to 4,299,124 amiA OKFHMN_22105: ypeA - GNAT family acetyltransferase, at 4,299,338 to 4,299,763 ypeA OKFHMN_22110: yfeZ - Inner membrane protein YfeZ, at 4,299,750 to 4,300,199 yfeZ Position (kb) 4298 4299 4300Strain fitness (log2 ratio) -1 0 1 2at 4297.354 kb on - strandat 4297.372 kb on + strandat 4297.372 kb on + strandat 4297.386 kb on - strandat 4297.426 kb on - strandat 4297.533 kb on + strand, within hemFat 4297.534 kb on - strand, within hemFat 4297.534 kb on - strand, within hemFat 4297.549 kb on - strand, within hemFat 4297.578 kb on + strand, within hemFat 4297.610 kb on + strand, within hemFat 4297.615 kb on - strand, within hemFat 4297.615 kb on - strand, within hemFat 4297.626 kb on + strand, within hemFat 4297.627 kb on - strand, within hemFat 4297.649 kb on - strand, within hemFat 4297.682 kb on + strand, within hemFat 4297.738 kb on - strand, within hemFat 4297.783 kb on + strand, within hemFat 4297.950 kb on + strand, within hemFat 4297.950 kb on + strand, within hemFat 4297.951 kb on - strand, within hemFat 4298.101 kb on + strand, within hemFat 4298.101 kb on + strand, within hemFat 4298.228 kb on - strandat 4298.530 kb on + strand, within amiAat 4298.714 kb on - strand, within amiAat 4298.837 kb on - strand, within amiAat 4299.009 kb on - strand, within amiAat 4299.138 kb on + strandat 4299.159 kb on + strandat 4299.160 kb on - strandat 4299.160 kb on - strandat 4299.162 kb on - strandat 4299.173 kb on + strandat 4299.210 kb on - strandat 4299.225 kb on - strandat 4299.334 kb on + strandat 4299.351 kb on + strandat 4299.445 kb on + strand, within ypeAat 4299.446 kb on - strand, within ypeAat 4299.548 kb on + strand, within ypeAat 4299.549 kb on - strand, within ypeAat 4299.549 kb on - strand, within ypeAat 4299.549 kb on - strand, within ypeAat 4299.805 kb on + strand, within yfeZat 4299.890 kb on + strand, within yfeZat 4299.912 kb on + strand, within yfeZat 4299.995 kb on + strand, within yfeZat 4300.023 kb on + strand, within yfeZat 4300.054 kb on + strand, within yfeZat 4300.055 kb on - strand, within yfeZat 4300.120 kb on + strand, within yfeZ

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,297,354 - +0.2
4,297,372 + -0.3
4,297,372 + -0.3
4,297,386 - +0.0
4,297,426 - +0.3
4,297,533 + hemF OKFHMN_22095 0.20 -0.7
4,297,534 - hemF OKFHMN_22095 0.20 -0.2
4,297,534 - hemF OKFHMN_22095 0.20 -1.0
4,297,549 - hemF OKFHMN_22095 0.22 +0.0
4,297,578 + hemF OKFHMN_22095 0.25 +0.2
4,297,610 + hemF OKFHMN_22095 0.29 -0.7
4,297,615 - hemF OKFHMN_22095 0.29 -0.4
4,297,615 - hemF OKFHMN_22095 0.29 +0.2
4,297,626 + hemF OKFHMN_22095 0.30 -0.3
4,297,627 - hemF OKFHMN_22095 0.31 -0.1
4,297,649 - hemF OKFHMN_22095 0.33 +0.5
4,297,682 + hemF OKFHMN_22095 0.37 -0.7
4,297,738 - hemF OKFHMN_22095 0.43 -0.5
4,297,783 + hemF OKFHMN_22095 0.48 -0.4
4,297,950 + hemF OKFHMN_22095 0.66 +0.9
4,297,950 + hemF OKFHMN_22095 0.66 -0.2
4,297,951 - hemF OKFHMN_22095 0.67 -0.1
4,298,101 + hemF OKFHMN_22095 0.83 -0.5
4,298,101 + hemF OKFHMN_22095 0.83 -1.3
4,298,228 - -0.4
4,298,530 + amiA OKFHMN_22100 0.32 -0.4
4,298,714 - amiA OKFHMN_22100 0.53 +0.7
4,298,837 - amiA OKFHMN_22100 0.67 +0.5
4,299,009 - amiA OKFHMN_22100 0.87 +0.0
4,299,138 + +0.6
4,299,159 + +1.5
4,299,160 - +0.2
4,299,160 - -0.1
4,299,162 - +0.7
4,299,173 + +0.3
4,299,210 - -0.1
4,299,225 - +0.6
4,299,334 + -0.1
4,299,351 + -0.4
4,299,445 + ypeA OKFHMN_22105 0.25 -0.3
4,299,446 - ypeA OKFHMN_22105 0.25 +0.1
4,299,548 + ypeA OKFHMN_22105 0.49 +1.5
4,299,549 - ypeA OKFHMN_22105 0.50 +0.7
4,299,549 - ypeA OKFHMN_22105 0.50 -0.8
4,299,549 - ypeA OKFHMN_22105 0.50 +0.7
4,299,805 + yfeZ OKFHMN_22110 0.12 +0.1
4,299,890 + yfeZ OKFHMN_22110 0.31 +0.9
4,299,912 + yfeZ OKFHMN_22110 0.36 +0.0
4,299,995 + yfeZ OKFHMN_22110 0.54 +0.1
4,300,023 + yfeZ OKFHMN_22110 0.61 -0.0
4,300,054 + yfeZ OKFHMN_22110 0.68 +0.3
4,300,055 - yfeZ OKFHMN_22110 0.68 +2.1
4,300,120 + yfeZ OKFHMN_22110 0.82 -0.4

Or see this region's nucleotide sequence