Strain Fitness in Escherichia coli ECRC100 around OKFHMN_21985

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaegA and nudK are separated by 67 nucleotidesnudK and ypfG are separated by 125 nucleotides OKFHMN_21980: aegA - formate-dependent uric acid utilization protein AegA, at 4,272,930 to 4,274,909 aegA OKFHMN_21985: nudK - GDP-mannose pyrophosphatase NudK, at 4,274,977 to 4,275,552 nudK OKFHMN_21990: ypfG - Uncharacterized protein YpfG, at 4,275,678 to 4,276,721 ypfG Position (kb) 4274 4275 4276Strain fitness (log2 ratio) -2 -1 0 1 2at 4273.996 kb on - strand, within aegAat 4274.166 kb on + strand, within aegAat 4274.441 kb on + strand, within aegAat 4274.441 kb on + strand, within aegAat 4274.719 kb on + strandat 4274.910 kb on + strandat 4274.911 kb on - strandat 4274.945 kb on + strandat 4274.945 kb on + strandat 4274.947 kb on + strandat 4274.948 kb on - strandat 4274.948 kb on - strandat 4275.025 kb on + strandat 4275.026 kb on - strandat 4275.045 kb on + strand, within nudKat 4275.045 kb on + strand, within nudKat 4275.046 kb on - strand, within nudKat 4275.050 kb on - strand, within nudKat 4275.050 kb on - strand, within nudKat 4275.331 kb on + strand, within nudKat 4275.331 kb on + strand, within nudKat 4275.331 kb on + strand, within nudKat 4275.333 kb on + strand, within nudKat 4275.334 kb on - strand, within nudKat 4275.334 kb on - strand, within nudKat 4275.783 kb on - strand, within ypfGat 4275.813 kb on - strand, within ypfGat 4275.813 kb on - strand, within ypfGat 4275.824 kb on + strand, within ypfGat 4275.881 kb on - strand, within ypfGat 4275.970 kb on + strand, within ypfGat 4276.003 kb on + strand, within ypfGat 4276.006 kb on + strand, within ypfGat 4276.006 kb on + strand, within ypfGat 4276.376 kb on + strand, within ypfGat 4276.395 kb on - strand, within ypfGat 4276.395 kb on - strand, within ypfGat 4276.414 kb on - strand, within ypfGat 4276.415 kb on + strand, within ypfG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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4,273,996 - aegA OKFHMN_21980 0.54 +1.0
4,274,166 + aegA OKFHMN_21980 0.62 -0.1
4,274,441 + aegA OKFHMN_21980 0.76 +0.5
4,274,441 + aegA OKFHMN_21980 0.76 -0.8
4,274,719 + -0.4
4,274,910 + +2.5
4,274,911 - -0.5
4,274,945 + -1.5
4,274,945 + +0.1
4,274,947 + +0.7
4,274,948 - +0.2
4,274,948 - +1.2
4,275,025 + +0.2
4,275,026 - +0.2
4,275,045 + nudK OKFHMN_21985 0.12 +0.1
4,275,045 + nudK OKFHMN_21985 0.12 +0.1
4,275,046 - nudK OKFHMN_21985 0.12 +1.1
4,275,050 - nudK OKFHMN_21985 0.13 +0.3
4,275,050 - nudK OKFHMN_21985 0.13 +0.2
4,275,331 + nudK OKFHMN_21985 0.61 +0.8
4,275,331 + nudK OKFHMN_21985 0.61 -0.3
4,275,331 + nudK OKFHMN_21985 0.61 +0.1
4,275,333 + nudK OKFHMN_21985 0.62 +0.2
4,275,334 - nudK OKFHMN_21985 0.62 +1.5
4,275,334 - nudK OKFHMN_21985 0.62 -0.5
4,275,783 - ypfG OKFHMN_21990 0.10 +0.3
4,275,813 - ypfG OKFHMN_21990 0.13 +1.1
4,275,813 - ypfG OKFHMN_21990 0.13 +0.0
4,275,824 + ypfG OKFHMN_21990 0.14 -0.5
4,275,881 - ypfG OKFHMN_21990 0.19 -0.5
4,275,970 + ypfG OKFHMN_21990 0.28 -0.3
4,276,003 + ypfG OKFHMN_21990 0.31 -0.8
4,276,006 + ypfG OKFHMN_21990 0.31 -1.5
4,276,006 + ypfG OKFHMN_21990 0.31 -0.3
4,276,376 + ypfG OKFHMN_21990 0.67 -0.5
4,276,395 - ypfG OKFHMN_21990 0.69 -1.1
4,276,395 - ypfG OKFHMN_21990 0.69 +0.4
4,276,414 - ypfG OKFHMN_21990 0.70 +2.0
4,276,415 + ypfG OKFHMN_21990 0.71 -1.8

Or see this region's nucleotide sequence