Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcaD and hcaB are separated by 9 nucleotides hcaB and hcaC overlap by 4 nucleotides hcaC and hcaF overlap by 1 nucleotides hcaF and hcaE overlap by 4 nucleotides
OKFHMN_21560: hcaD - phenylpropionate dioxygenase ferredoxin reductase subunit, at 4,180,829 to 4,182,031
hcaD
OKFHMN_21565: hcaB - 3-phenylpropionate-dihydrodiol/cinnamic acid-dihydrodiol dehydrogenase, at 4,182,041 to 4,182,853
hcaB
OKFHMN_21570: hcaC - bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit, at 4,182,850 to 4,183,170
hcaC
OKFHMN_21575: hcaF - 3-phenylpropionate/cinnamic acid dioxygenase subunit beta, at 4,183,170 to 4,183,688
hcaF
OKFHMN_21580: hcaE - 3-phenylpropionate/cinnamic acid dioxygenase subunit alpha, at 4,183,685 to 4,185,046
hcaE
Position (kb)
4182
4183
4184 Strain fitness (log2 ratio)
-2
-1
0
1 at 4181.907 kb on + strand, within hcaD at 4182.042 kb on + strand at 4182.156 kb on + strand, within hcaB at 4182.156 kb on + strand, within hcaB at 4182.157 kb on - strand, within hcaB at 4182.376 kb on + strand, within hcaB at 4182.428 kb on + strand, within hcaB at 4182.429 kb on - strand, within hcaB at 4182.514 kb on + strand, within hcaB at 4182.654 kb on + strand, within hcaB at 4182.902 kb on - strand, within hcaC at 4183.014 kb on - strand, within hcaC at 4183.036 kb on - strand, within hcaC at 4183.157 kb on + strand at 4183.158 kb on - strand at 4183.158 kb on - strand at 4183.158 kb on - strand at 4183.180 kb on - strand at 4183.191 kb on - strand at 4183.203 kb on + strand at 4183.292 kb on - strand, within hcaF at 4183.292 kb on - strand, within hcaF at 4183.292 kb on - strand, within hcaF at 4183.354 kb on - strand, within hcaF at 4183.395 kb on + strand, within hcaF at 4183.663 kb on - strand at 4183.663 kb on - strand at 4183.663 kb on - strand at 4183.708 kb on - strand at 4183.773 kb on + strand at 4183.774 kb on - strand at 4183.774 kb on - strand at 4183.804 kb on + strand at 4183.806 kb on + strand at 4183.810 kb on + strand at 4183.810 kb on + strand at 4183.811 kb on - strand at 4183.811 kb on - strand at 4183.811 kb on - strand at 4183.811 kb on - strand at 4183.811 kb on - strand at 4183.813 kb on + strand at 4184.029 kb on + strand, within hcaE at 4184.029 kb on + strand, within hcaE at 4184.030 kb on - strand, within hcaE at 4184.129 kb on - strand, within hcaE at 4184.129 kb on - strand, within hcaE at 4184.129 kb on - strand, within hcaE
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 4,181,907 + hcaD OKFHMN_21560 0.90 -1.9 4,182,042 + +0.2 4,182,156 + hcaB OKFHMN_21565 0.14 -0.1 4,182,156 + hcaB OKFHMN_21565 0.14 -0.1 4,182,157 - hcaB OKFHMN_21565 0.14 -1.6 4,182,376 + hcaB OKFHMN_21565 0.41 +0.2 4,182,428 + hcaB OKFHMN_21565 0.48 +0.2 4,182,429 - hcaB OKFHMN_21565 0.48 +0.2 4,182,514 + hcaB OKFHMN_21565 0.58 +1.0 4,182,654 + hcaB OKFHMN_21565 0.75 +0.6 4,182,902 - hcaC OKFHMN_21570 0.16 -0.6 4,183,014 - hcaC OKFHMN_21570 0.51 -0.1 4,183,036 - hcaC OKFHMN_21570 0.58 +0.2 4,183,157 + -0.2 4,183,158 - -0.1 4,183,158 - -1.3 4,183,158 - -1.3 4,183,180 - +0.0 4,183,191 - -0.4 4,183,203 + +0.6 4,183,292 - hcaF OKFHMN_21575 0.24 +0.3 4,183,292 - hcaF OKFHMN_21575 0.24 -0.4 4,183,292 - hcaF OKFHMN_21575 0.24 -0.3 4,183,354 - hcaF OKFHMN_21575 0.35 +0.3 4,183,395 + hcaF OKFHMN_21575 0.43 +0.2 4,183,663 - -0.5 4,183,663 - -0.2 4,183,663 - -0.3 4,183,708 - +0.1 4,183,773 + +0.4 4,183,774 - -0.4 4,183,774 - -0.5 4,183,804 + -0.0 4,183,806 + +0.2 4,183,810 + +0.9 4,183,810 + +0.3 4,183,811 - -0.0 4,183,811 - +0.4 4,183,811 - +0.9 4,183,811 - -1.0 4,183,811 - -0.0 4,183,813 + -0.0 4,184,029 + hcaE OKFHMN_21580 0.25 -0.8 4,184,029 + hcaE OKFHMN_21580 0.25 -1.2 4,184,030 - hcaE OKFHMN_21580 0.25 -0.0 4,184,129 - hcaE OKFHMN_21580 0.33 +0.2 4,184,129 - hcaE OKFHMN_21580 0.33 +1.3 4,184,129 - hcaE OKFHMN_21580 0.33 -1.3
Or see this region's nucleotide sequence