Strain Fitness in Escherichia coli ECRC100 around OKFHMN_10500

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaceF and aceE are separated by 14 nucleotidesaceE and pdhR are separated by 160 nucleotides OKFHMN_10495: aceF - pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase, at 1,971,116 to 1,973,008 aceF OKFHMN_10500: aceE - pyruvate dehydrogenase (acetyl-transferring), homodimeric type, at 1,973,023 to 1,975,686 aceE OKFHMN_10510: pdhR - pyruvate dehydrogenase complex transcriptional repressor PdhR, at 1,975,847 to 1,976,611 pdhR Position (kb) 1973 1974 1975 1976Strain fitness (log2 ratio) -2 -1 0 1at 1973.622 kb on - strand, within aceEat 1973.677 kb on - strand, within aceEat 1975.985 kb on - strand, within pdhRat 1975.985 kb on - strand, within pdhRat 1975.998 kb on - strand, within pdhRat 1976.096 kb on - strand, within pdhRat 1976.096 kb on - strand, within pdhRat 1976.258 kb on - strand, within pdhRat 1976.258 kb on - strand, within pdhRat 1976.258 kb on - strand, within pdhR

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,973,622 - aceE OKFHMN_10500 0.22 +1.7
1,973,677 - aceE OKFHMN_10500 0.25 +0.1
1,975,985 - pdhR OKFHMN_10510 0.18 +0.2
1,975,985 - pdhR OKFHMN_10510 0.18 +0.3
1,975,998 - pdhR OKFHMN_10510 0.20 +0.1
1,976,096 - pdhR OKFHMN_10510 0.33 -0.3
1,976,096 - pdhR OKFHMN_10510 0.33 -0.1
1,976,258 - pdhR OKFHMN_10510 0.54 +1.3
1,976,258 - pdhR OKFHMN_10510 0.54 -2.3
1,976,258 - pdhR OKFHMN_10510 0.54 +0.4

Or see this region's nucleotide sequence