Strain Fitness in Escherichia coli ECRC100 around OKFHMN_09980

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrnhA and yafS overlap by 4 nucleotidesyafS and gloB are separated by 33 nucleotidesgloB and mltD are separated by 209 nucleotides OKFHMN_09970: rnhA - ribonuclease HI, at 1,863,640 to 1,864,107 rnhA OKFHMN_09975: yafS - Uncharacterized protein YafS, at 1,864,104 to 1,864,826 yafS OKFHMN_09980: gloB - hydroxyacylglutathione hydrolase, at 1,864,860 to 1,865,615 gloB OKFHMN_09985: mltD - murein transglycosylase D, at 1,865,825 to 1,867,045 mltD Position (kb) 1864 1865 1866Strain fitness (log2 ratio) -2 -1 0 1 2at 1863.870 kb on + strand, within rnhAat 1864.083 kb on + strandat 1864.118 kb on + strandat 1864.223 kb on + strand, within yafSat 1864.230 kb on + strand, within yafSat 1864.231 kb on - strand, within yafSat 1864.231 kb on - strand, within yafSat 1864.251 kb on - strand, within yafSat 1864.314 kb on + strand, within yafSat 1864.315 kb on - strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.533 kb on - strand, within yafSat 1864.533 kb on - strand, within yafSat 1864.706 kb on - strand, within yafSat 1864.854 kb on + strandat 1864.854 kb on + strandat 1864.855 kb on - strandat 1864.860 kb on - strandat 1864.860 kb on - strandat 1864.875 kb on - strandat 1864.875 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.964 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.082 kb on + strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.106 kb on - strand, within gloBat 1865.122 kb on + strand, within gloBat 1865.171 kb on - strand, within gloBat 1865.224 kb on - strand, within gloBat 1865.315 kb on - strand, within gloBat 1865.334 kb on - strand, within gloBat 1865.342 kb on - strand, within gloBat 1865.342 kb on - strand, within gloBat 1865.358 kb on + strand, within gloBat 1865.360 kb on + strand, within gloBat 1865.390 kb on + strand, within gloBat 1865.390 kb on + strand, within gloBat 1865.422 kb on - strand, within gloBat 1865.607 kb on - strandat 1865.675 kb on - strandat 1865.940 kb on + strandat 1865.987 kb on + strand, within mltDat 1865.987 kb on + strand, within mltDat 1865.988 kb on - strand, within mltDat 1865.989 kb on + strand, within mltDat 1865.989 kb on + strand, within mltDat 1865.990 kb on - strand, within mltDat 1865.990 kb on - strand, within mltDat 1865.990 kb on - strand, within mltDat 1865.990 kb on - strand, within mltDat 1865.994 kb on - strand, within mltDat 1866.022 kb on + strand, within mltDat 1866.023 kb on - strand, within mltDat 1866.046 kb on - strand, within mltDat 1866.048 kb on + strand, within mltDat 1866.048 kb on + strand, within mltDat 1866.048 kb on + strand, within mltDat 1866.054 kb on + strand, within mltDat 1866.164 kb on + strand, within mltDat 1866.164 kb on + strand, within mltDat 1866.165 kb on - strand, within mltDat 1866.269 kb on + strand, within mltDat 1866.269 kb on + strand, within mltDat 1866.270 kb on - strand, within mltDat 1866.270 kb on - strand, within mltDat 1866.271 kb on + strand, within mltDat 1866.374 kb on + strand, within mltDat 1866.374 kb on + strand, within mltDat 1866.375 kb on - strand, within mltDat 1866.432 kb on + strand, within mltD

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,863,870 + rnhA OKFHMN_09970 0.49 -0.4
1,864,083 + +0.3
1,864,118 + +0.0
1,864,223 + yafS OKFHMN_09975 0.16 +0.1
1,864,230 + yafS OKFHMN_09975 0.17 -0.4
1,864,231 - yafS OKFHMN_09975 0.18 -1.1
1,864,231 - yafS OKFHMN_09975 0.18 +2.7
1,864,251 - yafS OKFHMN_09975 0.20 +0.1
1,864,314 + yafS OKFHMN_09975 0.29 +0.7
1,864,315 - yafS OKFHMN_09975 0.29 +0.5
1,864,532 + yafS OKFHMN_09975 0.59 +0.1
1,864,532 + yafS OKFHMN_09975 0.59 -0.1
1,864,532 + yafS OKFHMN_09975 0.59 -0.1
1,864,532 + yafS OKFHMN_09975 0.59 -1.1
1,864,533 - yafS OKFHMN_09975 0.59 +0.9
1,864,533 - yafS OKFHMN_09975 0.59 +0.0
1,864,706 - yafS OKFHMN_09975 0.83 +0.9
1,864,854 + +0.1
1,864,854 + -0.2
1,864,855 - -1.0
1,864,860 - -0.6
1,864,860 - -0.1
1,864,875 - +0.2
1,864,875 - +0.3
1,864,897 - +0.5
1,864,897 - -0.6
1,864,897 - +0.1
1,864,897 - -0.4
1,864,897 - -0.7
1,864,964 - gloB OKFHMN_09980 0.14 -0.6
1,865,006 - gloB OKFHMN_09980 0.19 -0.7
1,865,006 - gloB OKFHMN_09980 0.19 +0.3
1,865,006 - gloB OKFHMN_09980 0.19 +0.1
1,865,006 - gloB OKFHMN_09980 0.19 -0.4
1,865,082 + gloB OKFHMN_09980 0.29 +0.6
1,865,083 - gloB OKFHMN_09980 0.29 -0.9
1,865,083 - gloB OKFHMN_09980 0.29 +0.5
1,865,083 - gloB OKFHMN_09980 0.29 +0.1
1,865,083 - gloB OKFHMN_09980 0.29 -0.3
1,865,106 - gloB OKFHMN_09980 0.33 +1.0
1,865,122 + gloB OKFHMN_09980 0.35 +0.3
1,865,171 - gloB OKFHMN_09980 0.41 +1.3
1,865,224 - gloB OKFHMN_09980 0.48 +0.2
1,865,315 - gloB OKFHMN_09980 0.60 -0.6
1,865,334 - gloB OKFHMN_09980 0.63 +0.8
1,865,342 - gloB OKFHMN_09980 0.64 +0.1
1,865,342 - gloB OKFHMN_09980 0.64 -0.6
1,865,358 + gloB OKFHMN_09980 0.66 +0.3
1,865,360 + gloB OKFHMN_09980 0.66 -0.3
1,865,390 + gloB OKFHMN_09980 0.70 +1.1
1,865,390 + gloB OKFHMN_09980 0.70 -1.0
1,865,422 - gloB OKFHMN_09980 0.74 -1.5
1,865,607 - +0.0
1,865,675 - -1.7
1,865,940 + -0.3
1,865,987 + mltD OKFHMN_09985 0.13 +0.1
1,865,987 + mltD OKFHMN_09985 0.13 -0.2
1,865,988 - mltD OKFHMN_09985 0.13 +1.7
1,865,989 + mltD OKFHMN_09985 0.13 -0.3
1,865,989 + mltD OKFHMN_09985 0.13 -1.0
1,865,990 - mltD OKFHMN_09985 0.14 +0.1
1,865,990 - mltD OKFHMN_09985 0.14 +1.7
1,865,990 - mltD OKFHMN_09985 0.14 +0.7
1,865,990 - mltD OKFHMN_09985 0.14 -1.2
1,865,994 - mltD OKFHMN_09985 0.14 -0.1
1,866,022 + mltD OKFHMN_09985 0.16 -0.3
1,866,023 - mltD OKFHMN_09985 0.16 -1.8
1,866,046 - mltD OKFHMN_09985 0.18 +0.4
1,866,048 + mltD OKFHMN_09985 0.18 -0.6
1,866,048 + mltD OKFHMN_09985 0.18 -1.1
1,866,048 + mltD OKFHMN_09985 0.18 -0.2
1,866,054 + mltD OKFHMN_09985 0.19 +1.0
1,866,164 + mltD OKFHMN_09985 0.28 +0.0
1,866,164 + mltD OKFHMN_09985 0.28 +0.1
1,866,165 - mltD OKFHMN_09985 0.28 -0.0
1,866,269 + mltD OKFHMN_09985 0.36 -0.3
1,866,269 + mltD OKFHMN_09985 0.36 +0.5
1,866,270 - mltD OKFHMN_09985 0.36 +0.1
1,866,270 - mltD OKFHMN_09985 0.36 -0.5
1,866,271 + mltD OKFHMN_09985 0.37 -0.2
1,866,374 + mltD OKFHMN_09985 0.45 -0.3
1,866,374 + mltD OKFHMN_09985 0.45 -1.5
1,866,375 - mltD OKFHMN_09985 0.45 +0.1
1,866,432 + mltD OKFHMN_09985 0.50 -1.0

Or see this region's nucleotide sequence