Strain Fitness in Escherichia coli ECRC100 around OKFHMN_05950

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybjO and potI are separated by 59 nucleotidespotI and potH overlap by 4 nucleotidespotH and potG are separated by 9 nucleotides OKFHMN_05940: ybjO - Inner membrane protein YbjO, at 1,049,510 to 1,049,998 ybjO OKFHMN_05945: potI - putrescine ABC transporter permease PotI, at 1,050,058 to 1,050,903 potI OKFHMN_05950: potH - putrescine ABC transporter permease PotH, at 1,050,900 to 1,051,853 potH OKFHMN_05955: potG - putrescine ABC transporter ATP-binding subunit PotG, at 1,051,863 to 1,052,996 potG Position (kb) 1050 1051 1052Strain fitness (log2 ratio) -2 -1 0 1 2at 1050.018 kb on - strandat 1050.027 kb on + strandat 1050.028 kb on - strandat 1050.028 kb on - strandat 1050.028 kb on - strandat 1050.028 kb on - strandat 1050.032 kb on - strandat 1050.120 kb on - strandat 1050.170 kb on - strand, within potIat 1050.289 kb on - strand, within potIat 1050.394 kb on + strand, within potIat 1050.429 kb on + strand, within potIat 1050.443 kb on + strand, within potIat 1050.480 kb on + strand, within potIat 1050.651 kb on + strand, within potIat 1050.651 kb on + strand, within potIat 1051.142 kb on + strand, within potHat 1051.291 kb on + strand, within potHat 1051.291 kb on + strand, within potHat 1051.292 kb on - strand, within potHat 1051.399 kb on + strand, within potHat 1051.444 kb on - strand, within potHat 1051.447 kb on - strand, within potHat 1051.447 kb on - strand, within potHat 1051.454 kb on + strand, within potHat 1051.472 kb on + strand, within potHat 1051.657 kb on + strand, within potHat 1051.658 kb on - strand, within potHat 1051.693 kb on - strand, within potHat 1051.742 kb on + strand, within potHat 1051.805 kb on + strandat 1051.847 kb on + strandat 1051.937 kb on + strandat 1052.023 kb on + strand, within potGat 1052.034 kb on + strand, within potGat 1052.118 kb on + strand, within potGat 1052.284 kb on + strand, within potGat 1052.284 kb on + strand, within potGat 1052.487 kb on + strand, within potGat 1052.582 kb on + strand, within potGat 1052.582 kb on + strand, within potGat 1052.583 kb on - strand, within potGat 1052.583 kb on - strand, within potGat 1052.714 kb on + strand, within potGat 1052.714 kb on + strand, within potG

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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1,050,018 - +0.8
1,050,027 + +0.1
1,050,028 - +0.9
1,050,028 - -0.7
1,050,028 - -2.2
1,050,028 - -0.3
1,050,032 - +1.4
1,050,120 - +0.1
1,050,170 - potI OKFHMN_05945 0.13 -0.1
1,050,289 - potI OKFHMN_05945 0.27 -0.2
1,050,394 + potI OKFHMN_05945 0.40 -1.6
1,050,429 + potI OKFHMN_05945 0.44 -0.5
1,050,443 + potI OKFHMN_05945 0.46 -0.5
1,050,480 + potI OKFHMN_05945 0.50 +0.4
1,050,651 + potI OKFHMN_05945 0.70 -0.1
1,050,651 + potI OKFHMN_05945 0.70 +1.4
1,051,142 + potH OKFHMN_05950 0.25 +1.4
1,051,291 + potH OKFHMN_05950 0.41 -0.6
1,051,291 + potH OKFHMN_05950 0.41 +1.4
1,051,292 - potH OKFHMN_05950 0.41 +0.5
1,051,399 + potH OKFHMN_05950 0.52 +2.4
1,051,444 - potH OKFHMN_05950 0.57 -0.0
1,051,447 - potH OKFHMN_05950 0.57 -0.7
1,051,447 - potH OKFHMN_05950 0.57 -0.1
1,051,454 + potH OKFHMN_05950 0.58 -0.9
1,051,472 + potH OKFHMN_05950 0.60 -0.9
1,051,657 + potH OKFHMN_05950 0.79 +0.4
1,051,658 - potH OKFHMN_05950 0.79 +0.1
1,051,693 - potH OKFHMN_05950 0.83 +2.0
1,051,742 + potH OKFHMN_05950 0.88 +0.4
1,051,805 + +1.0
1,051,847 + -0.0
1,051,937 + -0.2
1,052,023 + potG OKFHMN_05955 0.14 -1.0
1,052,034 + potG OKFHMN_05955 0.15 -0.5
1,052,118 + potG OKFHMN_05955 0.22 +1.4
1,052,284 + potG OKFHMN_05955 0.37 +0.5
1,052,284 + potG OKFHMN_05955 0.37 +1.1
1,052,487 + potG OKFHMN_05955 0.55 +0.4
1,052,582 + potG OKFHMN_05955 0.63 +0.6
1,052,582 + potG OKFHMN_05955 0.63 -0.7
1,052,583 - potG OKFHMN_05955 0.63 -0.8
1,052,583 - potG OKFHMN_05955 0.63 +0.8
1,052,714 + potG OKFHMN_05955 0.75 +0.7
1,052,714 + potG OKFHMN_05955 0.75 -0.7

Or see this region's nucleotide sequence