Strain Fitness in Escherichia coli ECRC100 around OKFHMN_03920

Experiment: Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_03900 and OKFHMN_03905 are separated by 46 nucleotidesOKFHMN_03905 and OKFHMN_03910 are separated by 49 nucleotidesOKFHMN_03910 and OKFHMN_03915 overlap by 1 nucleotidesOKFHMN_03915 and yeeS are separated by 62 nucleotidesyeeS and OKFHMN_03925 are separated by 15 nucleotidesOKFHMN_03925 and OKFHMN_03930 are separated by 90 nucleotides OKFHMN_03900: OKFHMN_03900 - toxin, at 621,176 to 621,550 _03900 OKFHMN_03905: OKFHMN_03905 - antitoxin, at 621,597 to 621,971 _03905 OKFHMN_03910: OKFHMN_03910 - Phage-related protein, at 622,021 to 622,134 _03910 OKFHMN_03915: OKFHMN_03915 - DUF987 domain-containing protein, at 622,134 to 622,355 _03915 OKFHMN_03920: yeeS - UPF0758 protein YeeS, at 622,418 to 622,894 yeeS OKFHMN_03925: OKFHMN_03925 - restriction endonuclease, at 622,910 to 623,395 _03925 OKFHMN_03930: OKFHMN_03930 - DUF945 domain-containing protein, at 623,486 to 624,304 _03930 Position (kb) 622 623Strain fitness (log2 ratio) -1 0 1at 621.844 kb on + strand, within OKFHMN_03905at 622.166 kb on - strand, within OKFHMN_03915at 622.241 kb on + strand, within OKFHMN_03915at 622.243 kb on + strand, within OKFHMN_03915at 622.243 kb on + strand, within OKFHMN_03915at 622.275 kb on - strand, within OKFHMN_03915at 622.977 kb on + strand, within OKFHMN_03925at 622.996 kb on - strand, within OKFHMN_03925at 623.333 kb on - strand, within OKFHMN_03925at 623.401 kb on + strandat 623.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas09
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621,844 + OKFHMN_03905 0.66 -0.6
622,166 - OKFHMN_03915 0.14 +0.7
622,241 + OKFHMN_03915 0.48 -0.9
622,243 + OKFHMN_03915 0.49 -1.6
622,243 + OKFHMN_03915 0.49 +0.4
622,275 - OKFHMN_03915 0.64 +0.4
622,977 + OKFHMN_03925 0.14 +0.3
622,996 - OKFHMN_03925 0.18 -1.0
623,333 - OKFHMN_03925 0.87 +1.0
623,401 + +0.4
623,409 - +0.4

Or see this region's nucleotide sequence