Experiment: Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cgsD and cgsE are separated by 4 nucleotides cgsE and cgsF are separated by 24 nucleotides cgsF and cgsG are separated by 26 nucleotides cgsG and ycdZ are separated by 63 nucleotides
OKFHMN_03845: cgsD - transcriptional regulator CsgD, at 613,771 to 614,421
cgsD
OKFHMN_03850: cgsE - curli production assembly/transport protein CsgE, at 614,426 to 614,815
cgsE
OKFHMN_03855: cgsF - curli production assembly/transport protein CsgF, at 614,840 to 615,256
cgsF
OKFHMN_03860: cgsG - curli production assembly/transport protein CsgG, at 615,283 to 616,116
cgsG
OKFHMN_03865: ycdZ - Inner membrane protein YcdZ, at 616,180 to 616,701
ycdZ
Position (kb) 614
615
616 Strain fitness (log2 ratio) -1
0
1 at 614.206 kb on + strand, within cgsD at 614.527 kb on + strand, within cgsE at 614.601 kb on + strand, within cgsE at 615.139 kb on + strand, within cgsF at 615.254 kb on + strand at 615.443 kb on - strand, within cgsG at 615.687 kb on + strand, within cgsG at 615.943 kb on + strand, within cgsG at 616.093 kb on - strand at 616.152 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas09 remove 614,206 + cgsD OKFHMN_03845 0.67 +0.4 614,527 + cgsE OKFHMN_03850 0.26 +1.2 614,601 + cgsE OKFHMN_03850 0.45 +0.8 615,139 + cgsF OKFHMN_03855 0.72 +0.1 615,254 + +1.1 615,443 - cgsG OKFHMN_03860 0.19 -1.3 615,687 + cgsG OKFHMN_03860 0.48 -0.2 615,943 + cgsG OKFHMN_03860 0.79 -0.5 616,093 - +0.6 616,152 - -0.5
Or see this region's nucleotide sequence